FastQCFastQC Report
Sun 21 May 2023
EGAF00007847115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23605
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA7343.109510696886253No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA5772.444397373437831No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA5582.3639059521287864No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4341.8385935183223894No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4271.808938784155899No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4111.741156534632493No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3871.6394831603473838No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3521.4912094895149333No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3511.4869730989197205No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3221.3641177716585469No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3191.3514085998729082No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3161.3386994280872695No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2891.224316882016522No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2861.2116077102308833No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2641.1184071171362No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2561.084515992374497No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2421.0252065240415167No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2330.9870790086846006No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2320.9828426180893879No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2290.9701334463037493No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2280.9658970557085363No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2130.902351196780343No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2130.902351196780343No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2030.8599872908282143No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2030.8599872908282143No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2020.8557509002330014No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2000.8472781190425758No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.8303325566617241No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1930.8176233848760857No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1840.7794958695191697No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1780.7540775259478925No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1700.7201864011861894No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1690.7159500105909764No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.7074772294005507No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.6651133234484219No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1490.6312221986867189No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.6269858080915061No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1470.6227494174962931No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1470.6227494174962931No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1470.6227494174962931No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1450.6142766363058674No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1440.6100402457106545No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1430.6058038551154417No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1410.5973310739250159No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1400.593094683329803No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1380.5846219021393773No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1370.5803855115441643No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1350.5719127303537386No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1350.5719127303537386No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1320.5592035585681No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1300.5507307773776742No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1280.5422579961872485No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1280.5422579961872485No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1270.5380216055920356No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1260.5337852149968226No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1240.5253124338063969No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.5041304808303325No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1190.5041304808303325No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1100.4660029654734167No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1100.4660029654734167No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1040.4405846219021394No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1040.4405846219021394No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1030.4363482313069265No Hit
TCTACTACTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1020.43211184071171366No Hit
TCTACTACTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.43211184071171366No Hit
TCTACTACTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT990.41940266892607503No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG960.40669349714043634No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA960.40669349714043634No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA920.38974793475958486No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG910.38551154416437194No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA900.38127515356915903No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA880.3728023723787333No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA870.36856598178352046No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG840.3558568099978818No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA820.34738402880745606No Hit
TCTACTACTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT820.34738402880745606No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA790.3346748570218174No Hit
TCTACTACTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT780.33043846642660457No Hit
TCTACTACTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT760.3219656852361788No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG760.3219656852361788No Hit
TCTACTACTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT730.3092565134505401No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT730.3092565134505401No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC720.30502012285532726No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA680.2880745604744757No Hit
TCTACTACTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT670.2838381698792629No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA660.27960177928405No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA660.27960177928405No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA660.27960177928405No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA650.2753653886888371No Hit
TCTACTACTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT630.2668926074984113No Hit
TCTACTACTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT620.26265621690319846No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA560.23723787333192117No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT560.23723787333192117No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA560.23723787333192117No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.23300148273670834No Hit
TCTACTACTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT540.22876509214149543No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.22876509214149543No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA530.22452870154628257No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA530.22452870154628257No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.2202923109510697No Hit
TCTACTACTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT510.21605592035585683No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG500.21181952976064394No Hit
TCTACTACTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT500.21181952976064394No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC480.20334674857021817No Hit
TCTACTACTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT480.20334674857021817No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA470.1991103579750053No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA460.19487396737979243No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA460.19487396737979243No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT440.18640118618936666No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG430.18216479559415377No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC410.17369201440372803No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT400.16945562380851514No Hit
TCTACTACTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT400.16945562380851514No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA390.16521923321330229No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA390.16521923321330229No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC390.16521923321330229No Hit
TCTACTACTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT390.16521923321330229No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.15674645202287651No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT370.15674645202287651No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT370.15674645202287651No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT360.15251006142766363No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA350.14827367083245074No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC350.14827367083245074No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT350.14827367083245074No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG340.14403728023723786No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA340.14403728023723786No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG340.14403728023723786No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT340.14403728023723786No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC340.14403728023723786No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT330.139800889642025No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC330.139800889642025No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA320.13556449904681211No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC320.13556449904681211No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT310.13132810845159923No Hit
AATTGAGTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA310.13132810845159923No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT310.13132810845159923No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG310.13132810845159923No Hit
AATTGAGTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA300.12709171785638637No Hit
TCTACTACTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT300.12709171785638637No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT290.12285532726117349No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA290.12285532726117349No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCT290.12285532726117349No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT280.11861893666596059No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT280.11861893666596059No Hit
AATTGAGTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA280.11861893666596059No Hit
AATTGAGTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA270.11438254607074771No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT270.11438254607074771No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT260.11014615547553484No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC250.10590976488032197No Hit
TCTACTACTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT250.10590976488032197No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC240.10167337428510909No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC240.10167337428510909No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)