FastQCFastQC Report
Sun 21 May 2023
EGAF00007847126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34924
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12923.6994616882373155No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12733.6450578398808844No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10182.9149009277287825No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8792.5168938265948917No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG6301.803917077081663No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6271.7953269957622267No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5981.7122895430076737No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5811.6636124155308671No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5621.609208567174436No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5621.609208567174436No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4681.3400526858320927No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4441.2713320352766007No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4401.259878593517352No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4181.1968846638414843No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4161.19115794296186No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4091.1711144198831749No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4061.1625243385637383No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3951.1310273737258045No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3811.0909403275684344No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3721.065170083610125No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3721.065170083610125No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3711.0623067231703127No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3501.0021761539342573No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3480.9964494330546328No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.9563623868972627No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3250.9305921429389531No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3220.9220020616195167No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3050.8733249341427098No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2770.7931508418279692No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2710.7759706791890963No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2560.7330202725919139No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2510.718703470392853No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2450.7015233077539801No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2440.6986599473141679No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2420.6929332264345436No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2400.6872065055549192No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2380.6814797846752949No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2370.6786164242354829No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2330.6671629824762341No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2320.6642996220364219No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2290.6557095407169855No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2230.6385293780781125No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2190.6270759363188638No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2040.5841255297216814No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1970.5640820066429962No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1800.5154048791661895No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1780.5096781582865652No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1750.5010880769671286No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1720.49249799564769214No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1670.47818119344863136No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1670.47818119344863136No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1660.47531783300881914No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1640.46959111212919485No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.4581376703699461No Hit
TCTACTACTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1590.455274309930134No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1580.45241094949032185No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1540.44095750773107323No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1520.43523078685144884No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.4323674264116367No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1480.4237773450922002No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1440.41232390333295155No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1420.4065971824533272No Hit
TCTACTACTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1390.3980071011338907No Hit
TCTACTACTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1380.3951437406940786No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1370.3922803802542664No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1330.38082693849501775No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1330.38082693849501775No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1290.36937349673576914No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1280.36651013629595697No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1270.36364677585614474No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1240.3550566945367083No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1230.3521933340968961No Hit
TCTACTACTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.34073989233764745No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1160.33214981101821095No Hit
TCTACTACTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1160.33214981101821095No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1130.3235597296987745No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1090.31210628793952583No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1030.2949261253006528No Hit
TCTACTACTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1010.28919940442102854No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1000.2863360439812163No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA990.2834726835414042No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA990.2834726835414042No Hit
TCTACTACTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT970.27774596266177987No Hit
TCTACTACTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT960.2748826022219677No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA950.27201924178215553No Hit
TCTACTACTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT950.27201924178215553No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA890.2548390791432826No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT870.2491123582636582No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA860.24624899782384607No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT840.24052227694422174No Hit
TCTACTACTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT840.24052227694422174No Hit
TCTACTACTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT840.24052227694422174No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT830.23765891650440957No Hit
TCTACTACTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT830.23765891650440957No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA800.22906883518497306No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA780.22334211430534875No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA780.22334211430534875No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA770.22047875386553661No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.21761539342572442No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT750.21475203298591228No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA750.21475203298591228No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT730.20902531210628797No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.20902531210628797No Hit
TCTACTACTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT720.20616195166647577No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA720.20616195166647577No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.2032985912266636No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA700.20043523078685146No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT680.19470850990722713No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA670.19184514946741496No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA650.18611842858779062No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT650.18611842858779062No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.18611842858779062No Hit
TCTACTACTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT630.18039170770816632No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.18039170770816632No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT630.18039170770816632No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.18039170770816632No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA630.18039170770816632No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.17752834726835415No Hit
TCTACTACTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.16893826594891764No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC580.16607490550910547No Hit
TCTACTACTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT570.16321154506929333No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA560.16034818462948114No Hit
TCTACTACTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.15462146374985683No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA540.15462146374985683No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT540.15462146374985683No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.15175810331004466No Hit
TCTACTACTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT520.1488947428702325No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA520.1488947428702325No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA520.1488947428702325No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA510.14603138243042035No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA510.14603138243042035No Hit
TCTACTACTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT510.14603138243042035No Hit
TCTACTACTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT510.14603138243042035No Hit
TCTACTACTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC510.14603138243042035No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT500.14316802199060816No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA490.14030466155079602No Hit
TCTACTACTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC480.13744130111098385No Hit
TCTACTACTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC480.13744130111098385No Hit
TCTACTACTCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.13457794067117168No Hit
TCTACTACTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT470.13457794067117168No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA460.1317145802313595No Hit
TCTACTACTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT450.12885121979154737No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA430.12312449891192304No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA420.12026113847211087No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA410.11739777803229871No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA410.11739777803229871No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT410.11739777803229871No Hit
TCTACTACTCCGGACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCT400.11453441759248653No Hit
TCTACTACTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.11453441759248653No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA400.11453441759248653No Hit
TCTACTACTCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT400.11453441759248653No Hit
TCTACTACTCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT390.11167105715267438No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA390.11167105715267438No Hit
TCTACTACTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC380.10880769671286221No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT380.10880769671286221No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT380.10880769671286221No Hit
TCTACTACTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC380.10880769671286221No Hit
TCTACTACTCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT380.10880769671286221No Hit
TCTACTACTCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCT370.10594433627305005No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG360.10308097583323789No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGAGGGCCCCAAGCGTTA360.10308097583323789No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA360.10308097583323789No Hit
TCTACTACTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC360.10308097583323789No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA350.10021761539342573No Hit
TCTACTACTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT350.10021761539342573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)