FastQCFastQC Report
Sun 21 May 2023
EGAF00007847169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15768
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7834.965753424657534No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6704.249112125824454No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3182.0167427701674274No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3061.9406392694063925No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2871.820142059868087No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2851.8074581430745813No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2771.756722475900558No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2751.7440385591070522No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2641.67427701674277No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2631.667935058346017No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2601.6489091831557583No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2551.6171993911719937No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2451.5537798072044646No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2121.3444951801116183No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2031.2874175545408422No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1971.2493658041603246No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1941.2303399289700658No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1921.21765601217656No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1831.1605783866057837No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1791.1352105530187722No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1721.0908168442415018No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1711.0844748858447488No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1711.0844748858447488No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1671.059107052257737No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1591.0083713850837137No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1440.91324200913242No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1430.9069000507356672No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1320.837138508371385No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1270.8054287163876205No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1270.8054287163876205No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1260.7990867579908676No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1260.7990867579908676No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1240.7864028411973617No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1230.7800608828006088No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1200.76103500761035No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1140.7229832572298325No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1130.7166412988330796No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1120.7102993404363267No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1110.7039573820395738No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1090.6912734652460679No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1030.6532217148655505No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT930.5898021308980212No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT860.5454084221207509No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA840.532724505327245No Hit
AATCTGACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT840.532724505327245No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT840.532724505327245No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG840.532724505327245No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA830.5263825469304921No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT780.4946727549467275No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA780.4946727549467275No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT760.4819888381532217No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA760.4819888381532217No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT750.4756468797564688No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT750.4756468797564688No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG720.45662100456621No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA710.45027904616945713No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA700.4439370877727042No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA650.4122272957889396No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC630.3995433789954338No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA620.39320142059868085No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA590.3741755454084221No Hit
AATCTGACCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT590.3741755454084221No Hit
AATCTGACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT580.3678335870116692No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.35514967021816335No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT560.35514967021816335No Hit
AATCTGACCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT560.35514967021816335No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT540.3424657534246575No Hit
AATCTGACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT530.33612379502790457No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG530.33612379502790457No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT520.3297818366311517No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC510.3234398782343988No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA490.3107559614408929No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.30441400304414No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC480.30441400304414No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.28538812785388123No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.2663622526636225No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.2663622526636225No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC410.26002029426686957No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA410.26002029426686957No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC400.2536783358701167No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC400.2536783358701167No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA400.2536783358701167No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT400.2536783358701167No Hit
AATCTGACCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT390.24733637747336376No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC390.24733637747336376No Hit
AATCTGACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT380.24099441907661084No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA380.24099441907661084No Hit
AATCTGACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT370.23465246067985793No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA370.23465246067985793No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC360.228310502283105No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT360.228310502283105No Hit
AATCTGACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT350.2219685438863521No Hit
AATCTGACCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT350.2219685438863521No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT350.2219685438863521No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA340.21562658548959918No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC340.21562658548959918No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA340.21562658548959918No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT330.20928462709284626No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA330.20928462709284626No Hit
AATCTGACCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT320.20294266869609334No Hit
AATCTGACCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT310.19660071029934043No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT310.19660071029934043No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA310.19660071029934043No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA300.1902587519025875No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA300.1902587519025875No Hit
AATCTGACCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT290.1839167935058346No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT290.1839167935058346No Hit
AATCTGACCCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT290.1839167935058346No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA290.1839167935058346No Hit
AATCTGACCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT280.17757483510908167No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC280.17757483510908167No Hit
AATCTGACCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT280.17757483510908167No Hit
AATCTGACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT260.16489091831557584No Hit
AATCTGACCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT260.16489091831557584No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA260.16489091831557584No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA260.16489091831557584No Hit
AATCTGACCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT250.15854895991882292No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA250.15854895991882292No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA250.15854895991882292No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA250.15854895991882292No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA240.15220700152207No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA230.1458650431253171No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA230.1458650431253171No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT230.1458650431253171No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT230.1458650431253171No Hit
AATCTGACCCCGGACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCT230.1458650431253171No Hit
AATCTGACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT230.1458650431253171No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA230.1458650431253171No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA220.13952308472856417No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC220.13952308472856417No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC220.13952308472856417No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT220.13952308472856417No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT210.13318112633181126No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT210.13318112633181126No Hit
AATCTGACCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC210.13318112633181126No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA210.13318112633181126No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT200.12683916793505834No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA200.12683916793505834No Hit
AATCTGACCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT200.12683916793505834No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT200.12683916793505834No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA190.12049720953830542No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC190.12049720953830542No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG190.12049720953830542No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT180.1141552511415525No Hit
AATCTGACCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT180.1141552511415525No Hit
AATCTGACCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC180.1141552511415525No Hit
AATCTGACCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC180.1141552511415525No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA180.1141552511415525No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA180.1141552511415525No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA170.10781329274479959No Hit
AATCTGACCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT170.10781329274479959No Hit
AATCTGACCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT170.10781329274479959No Hit
AATCTGACCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT170.10781329274479959No Hit
AATCTGACCCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT170.10781329274479959No Hit
AATCTGACCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC170.10781329274479959No Hit
AATCTGACCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC170.10781329274479959No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA170.10781329274479959No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG160.10147133434804667No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA160.10147133434804667No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA160.10147133434804667No Hit
AATCTGACCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT160.10147133434804667No Hit
AATCTGACCCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT160.10147133434804667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)