FastQCFastQC Report
Sun 21 May 2023
EGAF00007847174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35365
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG13463.806022904001131No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11233.1754559592817753No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9572.7060653188180406No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8242.3299872755549274No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7722.182949243602432No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7192.0330835571893116No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6751.9086667609218153No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5921.6739714406899475No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5361.5156227908949527No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5271.4901739007493284No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4811.3601017955605825No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4711.3318252509543334No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4611.3035487063480842No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4301.215891418068712No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4161.1763042556199632No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4161.1763042556199632No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4021.1367170931712145No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3991.1282341297893397No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3961.119751166407465No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3851.088646967340591No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3781.0688533861162166No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3520.9953343701399688No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3420.9670578255337199No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3390.9585748621518451No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3360.9500918987699704No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3180.8991941184787219No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3070.8680899194118479No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2950.8341580658843489No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2940.831330411423724No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2850.8058815212780999No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2680.7578113954474763No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2560.7238795419199774No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2560.7238795419199774No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2540.7182242329987275No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2540.7182242329987275No Hit
TCTCTAAGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2440.6899476883924784No Hit
TCTCTAAGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2120.5994627456524813No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2090.5909797822706065No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2090.5909797822706065No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2030.574013855506857No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1940.5485649653612329No Hit
TCTCTAAGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1880.5315990385974834No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1880.5315990385974834No Hit
TCTCTAAGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1830.5174607662943589No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1830.5174607662943589No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1750.49483953060935953No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1720.4863565672274848No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1700.48070125830623495No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1640.4637353315424855No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1630.4609076770818606No Hit
TCTCTAAGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1630.4609076770818606No Hit
TCTCTAAGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1570.4439417503181112No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1560.4411140958574862No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1550.4382864413968613No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1540.4354587869362364No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1490.4213205146331118No Hit
TCTCTAAGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1440.4071822423299873No Hit
TCTCTAAGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1430.4043545878693624No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC1430.4043545878693624No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1400.39587162448748764No Hit
TCTCTAAGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1380.3902163155662378No Hit
TCTCTAAGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1380.3902163155662378No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1380.3902163155662378No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1320.37325038880248834No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1280.3619397709599887No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA1250.353456807578114No Hit
TCTCTAAGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1240.35062915311748905No Hit
TCTCTAAGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1240.35062915311748905No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1210.34214618973561434No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1200.3393185352749894No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC1190.33649088081436446No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1180.3336632263537396No Hit
TCTCTAAGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1170.3308355718931147No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1160.32800791743248975No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.32800791743248975No Hit
TCTCTAAGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1150.32518026297186486No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1130.319524954050615No Hit
TCTCTAAGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1120.3166972995899901No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1120.3166972995899901No Hit
TCTCTAAGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.2969037183656157No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1040.2940760639049908No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC1030.2912484094443659No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.2855931005231161No Hit
TCTCTAAGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1000.28276544606249115No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT980.2771101371412414No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA950.26862717375936657No Hit
TCTCTAAGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT950.26862717375936657No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA940.2657995192987417No Hit
TCTCTAAGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT940.2657995192987417No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA920.26014421037749186No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.2488335925349922No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC820.23186766577124274No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA810.22904001131061782No Hit
TCTCTAAGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT800.22621235684999294No Hit
TCTCTAAGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT780.2205570479287431No Hit
TCTCTAAGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT770.2177293934681182No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA760.2149017390074933No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC760.2149017390074933No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA740.20924643008624344No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC740.20924643008624344No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA740.20924643008624344No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA740.20924643008624344No Hit
TCTCTAAGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT730.20641877562561856No Hit
TCTCTAAGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC730.20641877562561856No Hit
TCTCTAAGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.20359112116499364No Hit
TCTCTAAGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC720.20359112116499364No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA710.2007634667043687No Hit
TCTCTAAGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT700.19793581224374382No Hit
TCTCTAAGACCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.19793581224374382No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA690.1951081577831189No Hit
TCTCTAAGACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT690.1951081577831189No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC690.1951081577831189No Hit
TCTCTAAGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC670.18945284886186908No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT670.18945284886186908No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT660.18662519440124417No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA660.18662519440124417No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC650.18379753994061926No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA640.18096988547999435No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA640.18096988547999435No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA630.17814223101936943No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA630.17814223101936943No Hit
TCTCTAAGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT630.17814223101936943No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA620.17531457655874452No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.17531457655874452No Hit
TCTCTAAGACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.1668316131768698No Hit
TCTCTAAGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC590.1668316131768698No Hit
TCTCTAAGACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT590.1668316131768698No Hit
TCTCTAAGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT590.1668316131768698No Hit
TCTCTAAGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT570.16117630425561996No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC550.15552099533437014No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.15552099533437014No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC540.15269334087374523No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC540.15269334087374523No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA540.15269334087374523No Hit
TCTCTAAGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT530.1498656864131203No Hit
TCTCTAAGACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACC530.1498656864131203No Hit
TCTCTAAGACCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.1498656864131203No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.1498656864131203No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.1442103774918705No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC500.14138272303124558No Hit
TCTCTAAGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT480.13572741410999575No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA480.13572741410999575No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA480.13572741410999575No Hit
TCTCTAAGACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.13572741410999575No Hit
TGTCCTTCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA470.13289975964937084No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC460.13007210518874593No Hit
TCTCTAAGACCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT460.13007210518874593No Hit
TCTCTAAGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT450.12724445072812102No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT440.1244167962674961No Hit
TCTCTAAGACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACC440.1244167962674961No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.1244167962674961No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.1244167962674961No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT430.1215891418068712No Hit
TGTCCTTCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG430.1215891418068712No Hit
TCTCTAAGACCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT430.1215891418068712No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA420.1187614873462463No Hit
TCTCTAAGACCGGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACC410.11593383288562137No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT410.11593383288562137No Hit
TCTCTAAGACCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACC390.11027852396437154No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA380.10745086950374665No Hit
TGTCCTTCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA370.10462321504312172No Hit
TGTCCTTCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA360.10179556058249682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)