FastQCFastQC Report
Sun 21 May 2023
EGAF00007847218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35736
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12303.441907320349228No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8312.3253861652115515No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7722.1602865457801657No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7152.0007835236176406No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6731.8832549809715695No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6521.8244907096485339No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5741.606223416162973No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5381.5054846653234832No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5361.4998880680546227No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5261.4719050817103203No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4981.3935527199462727No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4731.323595254085516No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4681.3096037609133646No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4421.2368479964181778No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4211.1780837250951421No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4151.1612939332885606No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4151.1612939332885606No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4131.1556973360197No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3911.0941347660622343No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3851.0773449742556527No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3811.0661517797179314No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3731.0437653906424893No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3550.9933960152227447No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3460.9682113275128721No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3450.9654130288784418No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3440.9626147302440116No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3290.9206402507275576No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3150.8814640698455339No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3100.8674725766733825No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2850.7975151108126259No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2800.7835236176404745No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2790.7807253190060444No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2720.7611372285650324No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2710.7583389299306023No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2660.7443474367584508No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2650.7415491381240206No Hit
TCTCTAAGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2490.6967763599731364No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2400.6715916722632639No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2230.6240205954779494No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2100.587642713230356No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2060.5764495186926349No Hit
TCTCTAAGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1930.5400716364450414No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1910.5344750391761809No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1900.5316767405417506No Hit
TCTCTAAGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1890.5288784419073204No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1880.5260801432728901No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1800.5036937541974479No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1790.5008954555630176No Hit
TCTCTAAGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1760.4925005596597269No Hit
TCTCTAAGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1700.4757107678531453No Hit
TCTCTAAGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1590.4449294828744123No Hit
TCTCTAAGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1580.4421311842399821No Hit
TCTCTAAGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1560.4365345869711216No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1560.4365345869711216No Hit
TCTCTAAGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1540.430937989702261No Hit
TCTCTAAGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1540.430937989702261No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1530.4281396910678308No Hit
TCTCTAAGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1430.4001567047235281No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1430.4001567047235281No Hit
TCTCTAAGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1410.3945601074546675No Hit
TCTCTAAGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1410.3945601074546675No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1360.3805686142825162No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1350.377770315648086No Hit
TCTCTAAGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1330.3721737183792254No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1290.36098052384150436No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1250.3497873293037833No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1200.33579583613163194No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1190.33299753749720173No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1180.3301992388627714No Hit
TCTCTAAGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1150.3218043429594806No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1140.31900604432505036No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1100.3078128497873293No Hit
TCTCTAAGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1090.30501455115289905No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1090.30501455115289905No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.30501455115289905No Hit
TCTCTAAGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1090.30501455115289905No Hit
TCTCTAAGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1050.293821356615178No Hit
TCTCTAAGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1040.2910230579807477No Hit
TCTCTAAGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.2798298634430266No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC990.27703156480859636No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA990.27703156480859636No Hit
TCTCTAAGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT980.2742332661741661No Hit
TCTCTAAGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT960.2686366689053056No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA950.2658383702708753No Hit
TCTCTAAGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT950.2658383702708753No Hit
TCTCTAAGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT940.26304007163644505No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC910.25464517573315426No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.24345198119543318No Hit
TCTCTAAGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT860.2406536825610029No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA840.23505708529214236No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG830.2322587866577121No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA830.2322587866577121No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA800.2238638907544213No Hit
TCTCTAAGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC800.2238638907544213No Hit
TCTCTAAGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT790.22106559211999105No Hit
TCTCTAAGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT780.2182672934855608No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA770.2154689948511305No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT760.21267069621670026No Hit
TCTCTAAGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC760.21267069621670026No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT740.2070740989478397No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.19867920304454892No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA710.19867920304454892No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.19867920304454892No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.19588090441011863No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA680.1902843071412581No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA650.1818894112379673No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC650.1818894112379673No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT630.1762928139691068No Hit
TCTCTAAGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT630.1762928139691068No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA620.1734945153346765No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT610.17069621670024623No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT610.17069621670024623No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA610.17069621670024623No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.16789791806581597No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA600.16789791806581597No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA600.16789791806581597No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT600.16789791806581597No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA590.1650996194313857No Hit
TCTCTAAGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT590.1650996194313857No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA580.16230132079695545No Hit
TCTCTAAGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT570.15950302216252518No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC560.15670472352809492No Hit
TCTCTAAGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC560.15670472352809492No Hit
TCTCTAAGACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC550.15390642489366466No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT530.1483098276248041No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC530.1483098276248041No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA520.14551152899037384No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC520.14551152899037384No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA520.14551152899037384No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA520.14551152899037384No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA520.14551152899037384No Hit
TCTCTAAGACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC520.14551152899037384No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA510.14271323035594358No Hit
TCTCTAAGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.14271323035594358No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC500.1399149317215133No Hit
TCTCTAAGACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC480.1343183344526528No Hit
TCTCTAAGACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC480.1343183344526528No Hit
TCTCTAAGACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC470.13152003581822252No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA470.13152003581822252No Hit
TCTCTAAGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.12872173718379223No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA460.12872173718379223No Hit
TCTCTAAGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.12592343854936197No Hit
TCTCTAAGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.12592343854936197No Hit
TCTCTAAGACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC450.12592343854936197No Hit
TCTCTAAGACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.12312513991493172No Hit
TCTCTAAGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT440.12312513991493172No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA430.12032684128050145No Hit
TCTCTAAGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT420.11752854264607118No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA420.11752854264607118No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG420.11752854264607118No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT420.11752854264607118No Hit
TCTCTAAGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT410.11473024401164092No Hit
TCTCTAAGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT410.11473024401164092No Hit
ATGACACTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA400.11193194537721066No Hit
TCTCTAAGACCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC400.11193194537721066No Hit
TCTCTAAGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACC390.1091336467427804No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA390.1091336467427804No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA380.10633534810835013No Hit
ATGACACTAGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG380.10633534810835013No Hit
TCTCTAAGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT380.10633534810835013No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC380.10633534810835013No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA370.10353704947391985No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG370.10353704947391985No Hit
TCTCTAAGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT370.10353704947391985No Hit
ATGACACTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA370.10353704947391985No Hit
TCTCTAAGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.10353704947391985No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA360.10073875083948958No Hit
TCTCTAAGACCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.10073875083948958No Hit
TCTCTAAGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT360.10073875083948958No Hit
ATGACACTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA360.10073875083948958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)