FastQCFastQC Report
Sun 21 May 2023
EGAF00007847268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39249
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA527413.4372850263701No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA32218.206578511554435No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA27897.105913526459274No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT17614.486738515630972No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA15443.9338581874697445No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12873.2790644347626694No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT11422.909628270783969No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT11232.8612193941246913No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8012.0408163265306123No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7421.890494025325486No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7341.8701113404163163No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6051.5414405462559555No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT6011.5312492038013708No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT4971.2662742999821652No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT4771.215317587709241No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4241.080282300185992No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT4011.0216820810721292No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3971.0114907386175442No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT3971.0114907386175442No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3610.9197686565262809No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3350.8535249305714796No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3190.8127595607531402No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2910.7414201635710463No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2830.7210374786618767No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2720.6930112869117685No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2340.5961935335932126No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2300.5860021911386277No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2070.5274019720247649No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2060.5248541364111187No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1960.4993757802746567No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1940.49428010904736425No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.4509669036153787No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1690.43058421870620905No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1550.39491452011516215No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.38472317766057734No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1400.35669698591046906No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.35414915029682287No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1380.3516013146831766No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1310.3337664653876532No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1270.32357512293306834No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA1260.32102728731942215No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1250.31847945170577596No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1230.31338378047848353No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1150.29300109556931386No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1090.2777140818874366No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA1050.26752273943285176No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1030.26242706820555933No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1030.26242706820555933No Hit
TCTCCGCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.25733139697826696No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT1010.25733139697826696No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1000.25478356136462077No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA940.2394965476827435No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT930.2369487120690973No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT880.22420953400086627No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT860.21911386277357384No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT840.2140181915462814No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG800.20382684909169663No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA790.2012790134780504No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA790.2012790134780504No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT780.1987311778644042No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA760.19363550663711177No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT750.19108767102346558No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA750.19108767102346558No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA750.19108767102346558No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA720.18344416418252693No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA720.18344416418252693No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC720.18344416418252693No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA710.18089632856888072No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA680.17325282172794212No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA680.17325282172794212No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC670.1707049861142959No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA630.16051364365971107No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG620.15796580804606486No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA610.15541797243241864No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG590.15032230120512624No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC580.14777446559148003No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA560.14267879436418762No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT530.135035287523249No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA530.135035287523249No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT520.1324874519096028No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCATGCT520.1324874519096028No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC510.1299396162959566No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT510.1299396162959566No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT510.1299396162959566No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA510.1299396162959566No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA510.1299396162959566No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT490.12484394506866417No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.12229610945501795No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA470.11974827384137175No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA470.11974827384137175No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA470.11974827384137175No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA470.11974827384137175No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT460.11720043822772555No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA460.11720043822772555No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA460.11720043822772555No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA440.11210476700043313No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC430.10955693138678692No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA420.1070090957731407No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA400.10191342454584831No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.10191342454584831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)