FastQCFastQC Report
Sun 21 May 2023
EGAF00007847276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31452
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA13954.435330026707364No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8062.562635126542032No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7932.5213023019203864No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6752.1461274322777566No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5571.7709525626351266No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5251.6692102251049217No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4881.5515706473356226No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4801.5261350629530714No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4771.5165967188096148No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4101.3035736996057483No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4011.2749586671753783No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3941.252702530840646No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3861.2272669464580948No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3751.192293017932087No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3671.1668574335495359No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3581.1382424011191656No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3551.128704056975709No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3521.1191657128322523No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3361.06829454406715No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3341.061935647971512No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3331.0587561999236934No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3331.0587561999236934No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3191.0142439272542287No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3191.0142439272542287No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3020.9601933104413074No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3000.9538344143456695No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2560.8139387002416381No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2430.7726058756199924No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2430.7726058756199924No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2280.7249141549027089No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2220.7058374666157955No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2220.7058374666157955No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2210.7026580185679766No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2200.6994785705201577No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2170.6899402263767009No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2160.6867607783288822No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2150.6835813302810632No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2140.6804018822332443No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2030.6454279537072364No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2010.6390690576115987No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1940.6168129212768664No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1920.6104540251812286No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1830.5818389927508585No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1790.5691212005595828No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1790.5691212005595828No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1680.534147272033575No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1670.530967823985756No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1660.5277883759379371No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1630.5182500317944805No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1560.49599389545974815No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1550.4928144474119293No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1530.4864555513162915No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1530.4864555513162915No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1530.4864555513162915No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1510.4800966552206537No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1480.470558311077197No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1440.45784051888592137No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.45784051888592137No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.45784051888592137No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1410.4483021747424647No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1390.4419432786468269No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1340.4260460384077324No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1320.4196871423120946No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1320.4196871423120946No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1320.4196871423120946No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1270.40378990207300014No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1260.4006104540251812No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1130.3592776294035355No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1130.3592776294035355No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1080.3433803891644411No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1070.34020094111662214No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1040.33066259697316547No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1010.32112425282970875No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1010.32112425282970875No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA990.31476535673407097No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA970.3084064606384332No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA970.3084064606384332No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT950.30204756454279535No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT930.2956886684471576No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA920.2925092203993387No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA920.2925092203993387No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA910.2893297723515198No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT910.2893297723515198No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT900.2861503243037009No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA890.28297087625588196No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA830.2638941879689686No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT800.2543558438255119No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT780.24799694772987407No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT780.24799694772987407No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG760.2416380516342363No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT750.23845860358641738No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA750.23845860358641738No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC720.22892025944296068No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC700.2225613633473229No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT700.2225613633473229No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT670.2130230192038662No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC650.2066641231082284No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT650.2066641231082284No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA640.20348467506040951No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA630.2003052270125906No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA630.2003052270125906No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA620.1971257789647717No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA610.19394633091695282No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG590.187587434821315No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA590.187587434821315No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA560.17804909067785832No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT560.17804909067785832No Hit
TCTCCGCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT560.17804909067785832No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA540.17169019458222054No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC540.17169019458222054No Hit
TCTCCGCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT530.16851074653440162No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC530.16851074653440162No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA520.16533129848658273No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT520.16533129848658273No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT510.16215185043876384No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA500.15897240239094493No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG500.15897240239094493No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA500.15897240239094493No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.15897240239094493No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC480.15261350629530715No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA460.14625461019966934No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.14625461019966934No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC450.14307516215185045No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.14307516215185045No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA440.13989571410403154No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA430.13671626605621265No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC420.13353681800839376No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.13353681800839376No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT420.13353681800839376No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT410.13035736996057484No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT410.13035736996057484No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA410.13035736996057484No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC410.13035736996057484No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.13035736996057484No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.13035736996057484No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA400.12717792191275595No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT390.12399847386493704No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA390.12399847386493704No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC390.12399847386493704No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG380.12081902581711815No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG380.12081902581711815No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC380.12081902581711815No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG380.12081902581711815No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA380.12081902581711815No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT370.11763957776929924No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG370.11763957776929924No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA370.11763957776929924No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG360.11446012972148034No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA360.11446012972148034No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG360.11446012972148034No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC360.11446012972148034No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC350.11128068167366145No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT350.11128068167366145No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT350.11128068167366145No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA350.11128068167366145No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT340.10810123362584255No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCGCACC340.10810123362584255No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA330.10492178557802365No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA330.10492178557802365No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC330.10492178557802365No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC320.10174233753020476No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA320.10174233753020476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)