FastQCFastQC Report
Sun 21 May 2023
EGAF00007847291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36480
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14744.040570175438597No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12543.4375000000000004No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG10522.883771929824561No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG8462.319078947368421No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7472.0476973684210527No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6301.7269736842105265No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5991.6419956140350875No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5611.537828947368421No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5611.537828947368421No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5561.5241228070175439No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5221.430921052631579No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4861.332236842105263No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4811.318530701754386No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4511.2362938596491229No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4501.2335526315789473No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4371.1979166666666667No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4071.1156798245614035No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3981.0910087719298245No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3941.0800438596491229No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3911.0718201754385965No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3871.0608552631578947No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3671.006030701754386No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3640.9978070175438596No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3540.9703947368421053No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3430.9402412280701754No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3350.918311403508772No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3340.9155701754385965No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3150.8634868421052633No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3000.8223684210526315No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2890.7922149122807018No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2610.7154605263157895No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2570.7044956140350878No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA2570.7044956140350878No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2550.6990131578947368No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2540.6962719298245614No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2500.6853070175438596No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2470.6770833333333334No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.6770833333333334No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2470.6770833333333334No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2380.6524122807017544No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2200.6030701754385964No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2150.5893640350877193No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA2120.581140350877193No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2080.5701754385964912No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2020.5537280701754386No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2000.5482456140350876No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1910.5235745614035088No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1900.5208333333333333No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1870.512609649122807No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1780.4879385964912281No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1760.4824561403508772No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1710.46875No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1670.45778508771929827No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1660.45504385964912286No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1600.43859649122807015No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1570.4303728070175439No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1560.4276315789473685No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1540.42214912280701755No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1530.4194078947368421No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1490.4084429824561403No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1420.3892543859649123No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1410.38651315789473684No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1390.38103070175438597No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1370.3755482456140351No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1370.3755482456140351No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1360.37280701754385964No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1360.37280701754385964No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.3536184210526316No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.3508771929824561No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1170.3207236842105263No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1150.3152412280701754No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.30701754385964913No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1110.3042763157894737No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1110.3042763157894737No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1090.2987938596491228No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1070.29331140350877194No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1010.2768640350877193No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1010.2768640350877193No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.2768640350877193No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1000.2741228070175438No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT990.2713815789473684No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA970.26589912280701755No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT910.2494517543859649No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT890.24396929824561406No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG870.23848684210526316No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT840.2302631578947368No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT830.22752192982456143No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA820.22478070175438597No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT810.2220394736842105No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA770.21107456140350878No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG760.20833333333333334No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA760.20833333333333334No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA760.20833333333333334No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT760.20833333333333334No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT750.20559210526315788No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA740.20285087719298245No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT730.20010964912280704No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA720.19736842105263158No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA710.19462719298245615No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA700.1918859649122807No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT700.1918859649122807No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA680.18640350877192982No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT670.1836622807017544No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA650.1781798245614035No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT650.1781798245614035No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT640.17543859649122806No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA630.17269736842105263No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA630.17269736842105263No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.17269736842105263No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA630.17269736842105263No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT620.1699561403508772No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC610.16721491228070176No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC600.1644736842105263No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT600.1644736842105263No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT600.1644736842105263No Hit
TCTCCGCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT590.1617324561403509No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.1617324561403509No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA580.15899122807017543No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC570.15625No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC560.15350877192982457No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.1480263157894737No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.1480263157894737No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC540.1480263157894737No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG530.14528508771929824No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA530.14528508771929824No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA520.1425438596491228No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA520.1425438596491228No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT520.1425438596491228No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC500.1370614035087719No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT490.1343201754385965No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA490.1343201754385965No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA460.12609649122807018No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT440.1206140350877193No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA440.1206140350877193No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA440.1206140350877193No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG430.11787280701754385No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.11787280701754385No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC420.1151315789473684No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC420.1151315789473684No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC400.10964912280701754No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA400.10964912280701754No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT390.10690789473684212No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG390.10690789473684212No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG390.10690789473684212No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT380.10416666666666667No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT380.10416666666666667No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT380.10416666666666667No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA380.10416666666666667No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT370.10142543859649122No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT370.10142543859649122No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT370.10142543859649122No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT370.10142543859649122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)