FastQCFastQC Report
Sun 21 May 2023
EGAF00007847300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35262
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16234.602688446486303No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10903.0911462764448983No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10623.0117406840224605No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8392.3793318586580456No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7222.047529918892859No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6691.897226476093245No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6171.7497589473087174No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5971.6930406670069762No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5431.539901310192275No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5271.4945266859508821No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5021.4236288355737055No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4981.4122851795133573No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4971.40944926549827No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4421.2534739946684816No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4361.2364585105779593No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4131.1712324882309568No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3991.131529692019738No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3801.0776473257330836No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3531.0010776473257332No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3480.9868980772502978No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3460.9812262492201237No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3370.9557030230843401No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3270.9273438829334695No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3190.904656570812773No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3000.8507742045261187No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2940.8337587204355963No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2860.8110714083149No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2770.7855481821791164No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2760.7827122681640294No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2740.7770404401338552No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2730.7742045261187681No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2620.7430094719528104No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2590.7345017299075493No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2520.7146503318019397No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2450.6947989336963304No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.680619363620895No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2360.6692757075605468No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2330.6607679655152856No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2130.6040496852135443No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2110.5983778571833702No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2060.5841982871079349No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2050.5813623730928478No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2040.5785264590777608No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2030.5756905450626737No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2030.5756905450626737No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1980.5615109749872383No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1860.5274800068061937No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1860.5274800068061937No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1850.5246440927911066No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1740.4934490386251489No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1730.49061312461006185No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1710.48494129657988766No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1700.4821053825648006No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.45374624241393No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1590.45091032839884293No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1540.43673075832340763No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1500.42538710226305937No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.4140434462027111No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1430.40553570415745No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.39419204809710173No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1380.3913561340820147No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1380.3913561340820147No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1350.38284839203675347No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1310.3715047359764052No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1260.35732516590096985No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1200.3403096818104475No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1190.3374737677953604No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1150.3261301117350122No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1130.3204582837048381No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.30060688559922866No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.29777097158414156No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1040.2949350575690545No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA990.28075548749361917No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT990.28075548749361917No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA990.28075548749361917No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA980.27791957347853213No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT950.2694118314332709No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC940.26657591741818387No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC900.2552322613578356No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT880.24956043332766148No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT870.24672451931257444No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG860.2438886052974874No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT850.2410526912824003No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA850.2410526912824003No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT820.23254494923713914No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA810.22970903522205208No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA810.22970903522205208No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT800.226873121206965No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT780.22120129317679088No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT780.22120129317679088No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA750.21269355113152968No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT710.20134989507118142No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA700.19851398105609439No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.19851398105609439No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT700.19851398105609439No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.19851398105609439No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA700.19851398105609439No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA670.1900062390108332No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC660.18717032499574612No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT650.18433441098065906No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT630.17866258295048493No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT630.17866258295048493No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC600.17015484090522376No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA600.17015484090522376No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT590.1673189268901367No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.1673189268901367No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA580.16448301287504963No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.16448301287504963No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC570.16164709885996256No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT570.16164709885996256No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC570.16164709885996256No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA560.1588111848448755No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC540.1531393568147014No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.1531393568147014No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC540.1531393568147014No Hit
TCTCCGCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT530.15030344279961433No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT530.15030344279961433No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA520.14746752878452726No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.14746752878452726No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT520.14746752878452726No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA500.14179570075435313No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA490.13895978673926607No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT490.13895978673926607No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA480.136123872724179No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT480.136123872724179No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA480.136123872724179No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT480.136123872724179No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT480.136123872724179No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT480.136123872724179No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG480.136123872724179No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC480.136123872724179No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT480.136123872724179No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.13328795870909194No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA470.13328795870909194No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA460.13045204469400487No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA450.1276161306789178No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA450.1276161306789178No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT450.1276161306789178No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT440.12478021666383074No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA440.12478021666383074No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA420.11910838863365664No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT420.11910838863365664No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT420.11910838863365664No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT420.11910838863365664No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC410.11627247461856957No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.1134365606034825No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC400.1134365606034825No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC400.1134365606034825No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT390.11060064658839544No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA380.10776473257330836No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.10776473257330836No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA380.10776473257330836No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC380.10776473257330836No Hit
TCTCCGCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT370.10492881855822132No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC360.10209290454313426No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATTCGAGCGTTA360.10209290454313426No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT360.10209290454313426No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT360.10209290454313426No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC360.10209290454313426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)