FastQCFastQC Report
Sun 21 May 2023
EGAF00007847301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35262
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15564.412682207475469No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA10332.9294991775849355No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9792.7763598207702342No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7752.1978333616924735No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7342.0815608870739037No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6001.7015484090522375No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5911.6760251829164539No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5591.5852759344336678No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5571.5796041064034938No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5481.5540808802677104No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4971.40944926549827No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4691.3300436730758325No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4651.3187000170154841No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4601.3045204469400489No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4511.2789972208042653No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4061.1513810901253474No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4021.1400374340649992No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3911.1088423798990414No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3891.1031705518688673No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3721.0549600136123873No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3691.046452271567126No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3350.950031195054166No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3330.9443593670239917No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3260.9245079689183824No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3170.8989847427825989No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3100.8791333446769893No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3100.8791333446769893No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2800.7940559242243775No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2770.7855481821791164No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2720.771368612103681No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2700.7656967840735069No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2610.7401735579377233No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2540.7203221598321139No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2480.7033066757415914No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2340.6636038795303726No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2270.6437524814247632No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2120.6012137711984573No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2100.5955419431682831No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2100.5955419431682831No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2050.5813623730928478No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2040.5785264590777608No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2020.5728546310475866No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1960.5558391469570643No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1950.5530032329419772No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1910.541659576881629No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1900.5388236628665418No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1840.5218081787760195No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1830.5189722647609325No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1820.5161363507458454No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1810.5133004367307583No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.49061312461006185No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1720.4877772105949748No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1680.47643355453462655No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1610.45658215642901706No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1580.4480744143837559No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1560.44240258635358176No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1490.42255118824797233No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1490.42255118824797233No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1440.40837161817253703No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1400.39702796211218877No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1380.3913561340820147No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1380.3913561340820147No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1300.3686688219613181No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1290.3658329079462311No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1280.362996993931144No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1230.3488174238557087No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1220.34598150984062165No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1170.3318019397651863No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.32896602575009926No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1150.3261301117350122No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1090.3091146276444898No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1050.29777097158414156No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.29777097158414156No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1040.2949350575690545No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1040.2949350575690545No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC980.27791957347853213No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT960.272247745448358No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA950.2694118314332709No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT920.26090408938800974No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT920.26090408938800974No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA910.2580681753729227No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT910.2580681753729227No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT890.2523963473427486No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA870.24672451931257444No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT850.2410526912824003No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG840.23821677726731327No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC810.22970903522205208No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA770.21836537916170382No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT770.21836537916170382No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG750.21269355113152968No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA740.20985763711644265No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT740.20985763711644265No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT730.20702172310135555No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.20702172310135555No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA730.20702172310135555No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT710.20134989507118142No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT700.19851398105609439No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC680.19284215302592025No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.19284215302592025No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT670.1900062390108332No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA670.1900062390108332No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA670.1900062390108332No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.1900062390108332No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT660.18717032499574612No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC660.18717032499574612No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT650.18433441098065906No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA650.18433441098065906No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA650.18433441098065906No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.181498496965572No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC630.17866258295048493No Hit
TCTCCGCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT620.17582666893539786No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT600.17015484090522376No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC590.1673189268901367No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT570.16164709885996256No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC570.16164709885996256No Hit
TCTCCGCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT570.16164709885996256No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA560.1588111848448755No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC550.15597527082978846No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT550.15597527082978846No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA540.1531393568147014No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA530.15030344279961433No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.15030344279961433No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA520.14746752878452726No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.1446316147694402No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC510.1446316147694402No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG510.1446316147694402No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.14179570075435313No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.14179570075435313No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA490.13895978673926607No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA490.13895978673926607No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC490.13895978673926607No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT490.13895978673926607No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC480.136123872724179No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT480.136123872724179No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA480.136123872724179No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC460.13045204469400487No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT460.13045204469400487No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.13045204469400487No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT440.12478021666383074No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.12478021666383074No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT440.12478021666383074No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC430.1219443026487437No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA430.1219443026487437No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA420.11910838863365664No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT420.11910838863365664No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT400.1134365606034825No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC400.1134365606034825No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA400.1134365606034825No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT400.1134365606034825No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA400.1134365606034825No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTA390.11060064658839544No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.10776473257330836No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT380.10776473257330836No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT380.10776473257330836No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT380.10776473257330836No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA370.10492881855822132No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT370.10492881855822132No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG370.10492881855822132No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT370.10492881855822132No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT370.10492881855822132No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT360.10209290454313426No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.10209290454313426No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT360.10209290454313426No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA360.10209290454313426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)