FastQCFastQC Report
Sun 21 May 2023
EGAF00007847328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40641
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA24496.025934401220442No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA20605.068772914052311No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA15633.845869934302798No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT12893.171673925346325No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA12743.134765384710022No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10552.5959006914199945No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8332.0496542900027066No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7081.742083118033513No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6741.658423759257892No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5611.3803794197977413No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5451.3410103097856845No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4961.2204424103737606No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4941.2155212716222534No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4591.1294013434708792No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4321.0629659703255334No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4141.0186757215619695No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3940.9694643340468984No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3820.9399375015378559No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT3710.9128712384045667No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3680.9054895302773062No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3520.8661204202652494No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3410.8390541571319604No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3410.8390541571319604No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3390.8341330183804532No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3340.8218301715016856No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3280.8070667552471642No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3220.7923033389926429No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3090.7603159371078467No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3050.7504736596048326No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2970.7307891045988042No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT2960.7283285352230506No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2930.7209468270957899No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2800.6889594252109938No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2680.6594325927019512No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2590.6372874683201692No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2560.6299057601929087No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2510.6176029133141409No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2500.6151423439383874No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2410.5929972195566054No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2320.5708520951748235No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT2180.5364041239142737No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2170.5339435545385203No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.4994955832779705No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2030.4994955832779705No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2030.4994955832779705No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2030.4994955832779705No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1900.46750818139317435No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1890.4650476120174208No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1890.4650476120174208No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1780.43798134888413176No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1780.43798134888413176No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1770.43552077950837825No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1710.42075736325385693No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1650.4059939469993356No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1610.39615166949632147No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1320.32479515759946853No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1270.31249231072070077No Hit
TCTCCGCACCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1270.31249231072070077No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1260.3100317413449472No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1260.3100317413449472No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1260.3100317413449472No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.3075711719691937No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1210.29772889446617945No Hit
TCTCCGCACCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1190.2928077557146724No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1180.2903471863389188No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1160.28542604758741175No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1140.2805049088359046No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1120.27558377008439755No Hit
TCTCCGCACCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1100.2706626313328904No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.2706626313328904No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1080.26574149258138335No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1070.2632809232056298No Hit
TCTCCGCACCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.25835978445412267No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1020.25097807632686203No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA970.2386752294480943No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC950.23375409069658717No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC920.22637238256932654No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA910.22391181319357298No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAACACGGAGGGTGCGAGCGTTA900.22145124381781944No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGGAGGGTGCGAGCGTTA890.21899067444206588No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.21899067444206588No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG880.21653010506631234No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA880.21653010506631234No Hit
TCTCCGCACCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT860.21160896631480525No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA860.21160896631480525No Hit
TCTCCGCACCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT850.20914839693905168No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.2042272581875446No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC810.1993061194360375No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA810.1993061194360375No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC810.1993061194360375No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG780.19192441130877685No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA770.1894638419330233No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC760.18700327255726976No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC760.18700327255726976No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAACACGGAGGGTGCGAGCGTTA750.1845427031815162No Hit
TCTCCGCACCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT740.18208213380576266No Hit
TCTCCGCACCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT730.1796215644300091No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC730.1796215644300091No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA720.17716099505425556No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC720.17716099505425556No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA710.174700425678502No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA700.17223985630274846No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC690.1697792869269949No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAACACGGAGGGTGCGAGCGTTA660.16239757879973427No Hit
TCTCCGCACCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT660.16239757879973427No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA640.15747644004822717No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA630.1550158706724736No Hit
TCTCCGCACCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.1550158706724736No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.1550158706724736No Hit
TCTCCGCACCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT580.14271302379370587No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA580.14271302379370587No Hit
TCTCCGCACCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC580.14271302379370587No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA580.14271302379370587No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA560.13779188504219878No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA560.13779188504219878No Hit
TCTCCGCACCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT550.1353313156664452No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC550.1353313156664452No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC540.13287074629069168No Hit
TCTCCGCACCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.13287074629069168No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC540.13287074629069168No Hit
TCTCCGCACCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT530.13041017691493811No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA530.13041017691493811No Hit
TCTCCGCACCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.12794960753918458No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.12548903816343102No Hit
TCTCCGCACCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT500.12302846878767748No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA500.12302846878767748No Hit
TCTCCGCACCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.12302846878767748No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT490.12056789941192392No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA480.11810733003617038No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA480.11810733003617038No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.11564676066041682No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA470.11564676066041682No Hit
TCTCCGCACCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.11564676066041682No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT450.11072562190890972No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA450.11072562190890972No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA450.11072562190890972No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA440.10826505253315617No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA440.10826505253315617No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA430.10580448315740262No Hit
TCTCCGCACCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC430.10580448315740262No Hit
TCTCCGCACCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.10580448315740262No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA430.10580448315740262No Hit
TCTCCGCACCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT430.10580448315740262No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA430.10580448315740262No Hit
TCTCCGCACCCGGACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCT430.10580448315740262No Hit
TCTCCGCACCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT420.10334391378164907No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA410.10088334440589553No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA410.10088334440589553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)