FastQCFastQC Report
Sun 21 May 2023
EGAF00007847377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41319
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12202.9526368014714777No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9722.352428664778915No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9292.2483603184975434No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7351.778842663181587No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6761.636051211307147No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6511.5755463588179772No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6361.5392434473244754No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6311.5271424768266415No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6261.5150415063288074No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6131.4835789830344395No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5831.4109731600474358No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5491.328686560662165No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5141.2439797671773276No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5111.2367191848786274No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA4851.1737941382898909No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4601.1132892858007213No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4581.1084488976015876No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4441.0745661802076527No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4371.0576248215106852No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4311.0431036569132845No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4050.980178610324548No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3990.9656574457271474No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3940.9535564752293134No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3800.9196737578353783No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3710.8978920109392774No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3690.8930516227401437No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3640.8809506522423097No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3630.8785304581427431No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3540.856748711246642No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3520.8519083230475084No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3170.767201529562671No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3100.7502601708657034No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2970.7187976475713352No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2930.7091168711730681No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2830.6849149301774002No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2820.6824947360778335No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2700.653452406883032No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2670.6461918245843317No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2520.6098889130908298No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2480.6002081366925627No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2470.597787942592996No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2420.5856869720951621No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2410.5832667779955952No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2360.5711658074977614No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2320.5614850310994942No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2310.5590648369999274No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2300.5566446429003606No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2230.5397032842033931No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2170.5251821196059924No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2150.5203417314068588No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2130.5155013432077252No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2050.49613979041119094No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2000.48403881991335707No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1970.4767782376146567No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1950.47193784941552314No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1910.462257073017256No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1870.45257629661898885No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1850.4477359084198553No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1820.44047532612115486No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1820.44047532612115486No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1810.43805513202158813No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1710.41385319102592033No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1700.4114329969263535No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1660.40175222052808635No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1660.40175222052808635No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1610.3896512500302524No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1590.38481086183111884No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1560.3775502795324185No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1540.3727098913332849No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1510.36544930903458456No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1510.36544930903458456No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1470.35576853263631747No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1460.3533483385367507No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1460.3533483385367507No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT1340.3243060093419492No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1330.3218858152423824No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1280.3097848447445485No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1230.2976838742467146No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1210.29284348604758104No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1200.29042329194801425No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1190.28800309784844746No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.2855829037488807No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.2831627096493139No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.2831627096493139No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1160.2807425155497471No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1130.27348193325104675No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1120.27106173915147996No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1100.2662213509523464No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT1090.2638011568527796No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1080.2613809627532128No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1060.25654057455407925No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1040.2517001863549457No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1020.2468597981558121No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.2468597981558121No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1010.24443960405624532No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.24443960405624532No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.24201940995667853No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT990.23959921585711175No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA980.237179021757545No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA970.23475882765797815No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA970.23475882765797815No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT960.23233863355841136No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT950.22991843945884458No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT920.22265785716014422No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT900.21781746896101067No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.21781746896101067No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT880.2129770807618771No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT850.20571649846317674No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC850.20571649846317674No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA850.20571649846317674No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT850.20571649846317674No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC820.1984559161644764No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.19361552796534282No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT790.19119533386577603No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC790.19119533386577603No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT780.18877513976620924No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC770.18635494566664246No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA750.18151455746750889No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA750.18151455746750889No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT750.18151455746750889No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG700.16941358696967498No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC690.1669933928701082No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC690.1669933928701082No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA690.1669933928701082No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT690.1669933928701082No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT680.16457319877054138No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA670.1621530046709746No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.1621530046709746No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACT660.1597328105714078No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA650.15731261647184103No Hit
TCATGCCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT650.15731261647184103No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT650.15731261647184103No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA630.15247222827270746No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.1476318400735739No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC610.1476318400735739No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT610.1476318400735739No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA610.1476318400735739No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC610.1476318400735739No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC600.14521164597400713No Hit
TCGAATTACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG600.14521164597400713No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT600.14521164597400713No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT550.1331106754761732No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.1306904813766064No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA540.1306904813766064No Hit
TCATGCCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT530.12827028727703962No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT530.12827028727703962No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT530.12827028727703962No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC520.12585009317747284No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT510.12342989907790605No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG510.12342989907790605No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC510.12342989907790605No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG500.12100970497833927No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC500.12100970497833927No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA500.12100970497833927No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT500.12100970497833927No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT500.12100970497833927No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC490.1185895108787725No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACT480.11616931677920568No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.1137491226796389No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA470.1137491226796389No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC450.10890873448050534No Hit
TCATGCCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT450.10890873448050534No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA450.10890873448050534No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.10890873448050534No Hit
TCGAATTACGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG440.10648854038093855No Hit
TCGAATTACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG440.10648854038093855No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACT440.10648854038093855No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.10648854038093855No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.10648854038093855No Hit
TCGAATTACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA420.10164815218180498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)