FastQCFastQC Report
Sun 21 May 2023
EGAF00007847384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49063
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12512.5497829321484624No Hit
TAAGACATGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10452.1299145995964373No Hit
TAAGACATGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10272.0932270753928623No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10202.07895970486925No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10172.0728451175019873No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8471.7263518333571124No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8451.7222754417789372No Hit
TAAGACATGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8431.7181990502007625No Hit
TAAGACATGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8061.6427858060045246No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7841.5979454986445998No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7731.5755253449646371No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG7511.5306850376047123No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7331.4939975134011372No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7321.4919593176120498No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6991.4246988565721623No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6781.3818967450013249No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6591.3431710250086624No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6201.2636813892342498No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5711.1638097955689624No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5461.112854900841775No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5421.1047021176854248No Hit
TAAGACATGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5361.0924729429509No Hit
TAAGACATGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5141.0476326355909749No Hit
TAAGACATGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5041.0272506777000998No Hit
TAAGACATGCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5001.0190978945437499No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4991.0170596987546623No Hit
TAAGACATGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4911.0007541324419622No Hit
TAAGACATGCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4850.9885249577074373No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4580.933493671402075No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4370.8906915598312373No Hit
TAAGACATGCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4320.8805005808858No Hit
TAAGACATGCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT4190.8540040356276624No Hit
TAAGACATGCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4120.8397366651040499No Hit
TAAGACATGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3840.7826671830095999No Hit
TAAGACATGCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT3640.7419032672278499No Hit
TAAGACATGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3440.7011393514461No Hit
TAAGACATGCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3370.6868719809224875No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3140.6399934777734749No Hit
TAAGACATGCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3010.6134969325153374No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2840.5788476041008499No Hit
TAAGACATGCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2750.5605038419990624No Hit
TAAGACATGCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2740.558465646209975No Hit
TAAGACATGCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2690.5482746672645374No Hit
TAAGACATGCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2670.5441982756863625No Hit
TAAGACATGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2520.5136253388500499No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2370.48305240201373745No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2300.46878503149012496No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2260.460632248333775No Hit
TAAGACATGCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2190.44636487781016243No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2050.41783013676293745No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2020.41171554939567495No Hit
TAAGACATGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1980.40356276623932497No Hit
TAAGACATGCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1950.39744817887206246No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1880.38318080834845No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1870.38114261255936244No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1820.37095163361392497No Hit
TAAGACATGCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1770.36076065466848745No Hit
TAAGACATGCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1750.3566842630903125No Hit
TAAGACATGCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.35260787151213746No Hit
TAAGACATGCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.35260787151213746No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1710.34853147993396244No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1690.3444550883557875No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1680.34241689256669994No Hit
TAAGACATGCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1650.3363023051994375No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1640.33426410941034995No Hit
TAAGACATGCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1620.330187717832175No Hit
TAAGACATGCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1600.32611132625399997No Hit
TAAGACATGCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1600.32611132625399997No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1590.3240731304649125No Hit
TAAGACATGCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1570.31999673888673746No Hit
TAAGACATGCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1510.30776756415221246No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1480.30165297678494996No Hit
TAAGACATGCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1440.29350019362859997No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1440.29350019362859997No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1440.29350019362859997No Hit
TAAGACATGCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1410.28738560626133747No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1400.28534741047225No Hit
TAAGACATGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1390.28330921468316245No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1390.28330921468316245No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1390.28330921468316245No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1380.28127101889407496No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1370.2792328231049875No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1360.27719462731589994No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1360.27719462731589994No Hit
TAAGACATGCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1320.26904184415954996No Hit
TAAGACATGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1320.26904184415954996No Hit
TAAGACATGCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1290.26292725679228746No Hit
TAAGACATGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1270.2588508652141125No Hit
TAAGACATGCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1270.2588508652141125No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1180.24050710311232498No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1170.23846890732323747No Hit
TAAGACATGCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.23846890732323747No Hit
TAAGACATGCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1130.2303161241668875No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1120.22827792837779998No Hit
TAAGACATGCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1120.22827792837779998No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1110.22623973258871247No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1100.22420153679962498No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1090.22216334101053747No Hit
TAAGACATGCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.2140105578541875No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1050.2140105578541875No Hit
TAAGACATGCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1040.21197236206509998No Hit
TAAGACATGCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1020.20789597048692499No Hit
TAAGACATGCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC990.20178138311966248No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA980.19974318733057497No Hit
TAAGACATGCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC970.1977049915414875No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA960.19566679575239998No Hit
TAAGACATGCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC910.18547581680696248No Hit
TAAGACATGCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT900.18343762101787497No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA890.1813994252287875No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA880.17936122943969998No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA870.17732303365061247No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA860.175284837861525No Hit
TAAGACATGCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC850.17324664207243748No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA850.17324664207243748No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA830.1691702504942625No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA810.1650938589160875No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA800.16305566312699998No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA790.16101746733791247No Hit
TAAGACATGCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT780.158979271548825No Hit
TAAGACATGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT770.15694107575973748No Hit
TAAGACATGCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT770.15694107575973748No Hit
TAAGACATGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT760.15490287997065No Hit
TAAGACATGCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT750.1528646841815625No Hit
TAAGACATGCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.15082648839247498No Hit
TAAGACATGCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC740.15082648839247498No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA740.15082648839247498No Hit
TAAGACATGCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT740.15082648839247498No Hit
TAAGACATGCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.1487882926033875No Hit
TAAGACATGCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT720.14675009681429999No Hit
TAAGACATGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT720.14675009681429999No Hit
TAAGACATGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC700.142673705236125No Hit
TAAGACATGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT690.14063550944703748No Hit
TAAGACATGCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.13859731365794997No Hit
ACAACTACTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA650.1324827262906875No Hit
TAAGACATGCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT640.1304445305016No Hit
TAAGACATGCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.1304445305016No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA640.1304445305016No Hit
TAAGACATGCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC630.12840633471251248No Hit
TAAGACATGCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT630.12840633471251248No Hit
TAAGACATGCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT620.126368138923425No Hit
TAAGACATGCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT600.12229174734524999No Hit
TAAGACATGCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC600.12229174734524999No Hit
TAAGACATGCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT590.12025355155616249No Hit
TAAGACATGCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT580.11821535576707498No Hit
TAAGACATGCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT580.11821535576707498No Hit
TAAGACATGCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT570.11617715997798748No Hit
TAAGACATGCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.11413896418889999No Hit
TAAGACATGCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC560.11413896418889999No Hit
TAAGACATGCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC560.11413896418889999No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA550.11210076839981249No Hit
TAAGACATGCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.110062572610725No Hit
TAAGACATGCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.110062572610725No Hit
TAAGACATGCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT510.10394798524346249No Hit
TAAGACATGCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT510.10394798524346249No Hit
TAAGACATGCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC510.10394798524346249No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA510.10394798524346249No Hit
TAAGACATGCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT500.10190978945437498No Hit
ACAACTACTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA500.10190978945437498No Hit
ACAACTACTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA500.10190978945437498No Hit
ACAACTACTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA500.10190978945437498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)