FastQCFastQC Report
Sun 21 May 2023
EGAF00007847392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39407
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA32578.265029055751516No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA30177.656000203009618No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT11182.8370594056893443No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT9322.365062044814373No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9292.3574491841550995No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9022.2889334382216355No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA8552.169665287893014No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA8002.030096175806329No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT7721.9590428096531074No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5381.365239678229756No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5341.3550891973507244No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5241.3297129951531454No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4911.2459715279011343No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4861.2332834268023447No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4811.2205953257035553No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4741.20283198416525No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4721.197756743725734No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4541.1520795797700916No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4311.0937143147156596No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4231.0734133529575962No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4191.0632628720785648No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4001.0150480879031645No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3971.0074352272438907No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3870.9820590250463116No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3510.8907046971350268No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3130.7942751287842262No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3090.7841246479051945No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2990.7587484457076155No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2810.713071281751973No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2650.6724693582358464No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2480.629329814499962No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2460.624254574060446No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.6090288527418987No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2360.598878371862867No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2330.5912655112035933No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2290.5811150303245616No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2110.5354378663689192No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2060.5227497652701297No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1980.5024488035120664No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1950.4948359428527926No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1830.46438450021569777No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1790.4542340193366661No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1720.4364706777983607No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1620.4110944756007816No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1570.398406374501992No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1540.3907935138427183No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1520.38571827340320247No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1430.3628796914253813No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA1420.3603420712056234No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1410.3578044509858655No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1390.35272921054634965No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1390.35272921054634965No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1380.35019159032659175No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1360.3451163498870759No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1330.33750348922780216No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1260.31974014768949677No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1250.31720252746973887No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1250.31720252746973887No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1230.312127287030223No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1160.2943639454919177No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1150.29182632527215974No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1150.29182632527215974No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1140.28928870505240184No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1100.27913822417337025No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.2766006039536123No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1080.2740629837338544No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1040.26391250285482276No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1020.2588372624153069No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT970.24614916131651737No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT930.23599868043748573No Hit
TCATGCCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT880.22331057933869616No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT870.22077295911893824No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA870.22077295911893824No Hit
TCATGCCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT850.21569771867942242No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT840.21316009845966452No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA830.2106224782399066No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA830.2106224782399066No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT810.2055472378003908No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC790.20047199736087498No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA770.19539675692135916No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.1903215164818433No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC740.18778389626208541No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA730.1852462760423275No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA720.1827086558225696No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA720.1827086558225696No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC710.1801710356028117No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT690.17509579516329588No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA690.17509579516329588No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG690.17509579516329588No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT680.17255817494353795No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA680.17255817494353795No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC670.17002055472378005No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA650.1649453142842642No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.1649453142842642No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC640.1624076940645063No Hit
TCATGCCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT640.1624076940645063No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT630.15987007384474838No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA620.1573324536249905No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA620.1573324536249905No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.15479483340523256No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT600.15225721318547467No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA600.15225721318547467No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT590.14971959296571674No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA590.14971959296571674No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.14464435252620092No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA570.14464435252620092No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG560.14210673230644302No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC560.14210673230644302No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA550.13956911208668513No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA550.13956911208668513No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG550.13956911208668513No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT550.13956911208668513No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA550.13956911208668513No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT540.1370314918669272No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA540.1370314918669272No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.13449387164716928No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT520.13195625142741138No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT520.13195625142741138No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC510.12941863120765346No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC500.12688101098789556No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG500.12688101098789556No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA490.12434339076813763No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG490.12434339076813763No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTG480.12180577054837972No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.11926815032862181No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA470.11926815032862181No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.11419290988910599No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT450.11419290988910599No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA450.11419290988910599No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA430.10911766944959017No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA430.10911766944959017No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC430.10911766944959017No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT420.10658004922983226No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA420.10658004922983226No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA410.10404242901007434No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC410.10404242901007434No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT410.10404242901007434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)