FastQCFastQC Report
Sun 21 May 2023
EGAF00007847394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36251
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA22376.170864252020634No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19705.434332845990456No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7652.1102866127830957No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6721.853741965738876No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6651.8344321535957628No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6491.7902954401257898No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6171.7020220131858432No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6161.6992634685939696No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5821.6054729524702767No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5771.59168022951091No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5411.4923726242034703No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5271.4537529999172436No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5261.4509944553253704No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4931.3599624837935504No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4721.3020330473642105No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4421.2192767096080108No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4051.1172105597086976No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3991.1006592921574578No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3931.0841080246062178No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA3911.0785909354224712No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3490.9627320625637913No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3490.9627320625637913No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA3460.9544564287881714No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3430.9461807950125514No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3400.9379051612369315No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3340.9213538936856913No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3280.9048026261344514No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3140.8661830018482249No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3120.8606659126644782No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3040.8385975559294916No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2790.7696339411326584No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2780.7668753965407851No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2750.758599762765165No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2740.7558412181732918No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2710.7475655843976717No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.6151554439877521No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.5323991062315523No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1920.5296405616396789No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1830.5048136603128189No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1810.4992965711290723No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1760.4855038481697056No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1720.47446966980221233No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1670.46067694684284566No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1670.46067694684284566No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1650.45515985765909905No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1620.4468842238834791No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1610.4441256792916058No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1560.4303329563322391No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1520.4192987779647458No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1490.4110231441891258No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1470.4055060550053792No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1440.3972304212297592No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1400.3861962428622659No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1380.38067915367851923No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1370.3779206090866459No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1360.3751620644947726No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1290.3558522523516593No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1280.35309370775978594No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1250.344818073984166No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1220.33654244020854596No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA1220.33654244020854596No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1210.33378389561667265No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1180.32550826184105264No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1150.3172326280654327No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1120.3089569942898127No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.29792281592231934No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1070.29516427133044604No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1070.29516427133044604No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1060.29240572673857274No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1010.2786130037792061No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC980.2703373700035861No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT960.26482028081983944No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT950.26206173622796614No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT950.26206173622796614No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT930.2565446470442195No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT920.2537861024523462No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT910.2510275578604728No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG910.2510275578604728No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT900.24826901326859951No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT900.24826901326859951No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT880.2427519240848528No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA880.2427519240848528No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA870.2399933794929795No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC850.23447629030923287No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT850.23447629030923287No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG840.2317177457173595No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT830.2289592011254862No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT830.2289592011254862No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA830.2289592011254862No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA810.22344211194173955No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCAGGTAGTTAGACCAGCAC780.21516647816611956No Hit
TCATGCCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT780.21516647816611956No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA770.2124079335742462No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA770.2124079335742462No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.2124079335742462No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA770.2124079335742462No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT770.2124079335742462No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA760.2096493889823729No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCAGGTAGTTAGACCAGCAC750.20689084439049957No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.19585666602300625No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA710.19585666602300625No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCAGGTAGTTAGACCAGCAC710.19585666602300625No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT700.19309812143113295No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA690.19033957683925962No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.19033957683925962No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT680.1875810322473863No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA670.18482248765551296No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCAGGTAGTTAGACCAGCAC670.18482248765551296No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA660.18206394306363963No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA650.1793053984717663No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.1793053984717663No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA630.17378830928801964No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCAGGTAGTTAGACCAGCAC630.17378830928801964No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT630.17378830928801964No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCAGGTAGTTAGACCAGCAC620.1710297646961463No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC610.16827122010427298No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.16827122010427298No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC610.16827122010427298No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.16551267551239965No Hit
TCATGCCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT590.16275413092052632No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA590.16275413092052632No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.16275413092052632No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC570.1572370417367797No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA570.1572370417367797No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC570.1572370417367797No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT560.15447849714490636No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC560.15447849714490636No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.15447849714490636No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.14896140796115967No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.14896140796115967No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCAGGTAGTTAGACCAGCAC520.14344431877741304No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT520.14344431877741304No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCAGGTAGTTAGACCAGCAC520.14344431877741304No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT510.1406857741855397No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA510.1406857741855397No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTA510.1406857741855397No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.13792722959366638No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT500.13792722959366638No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT500.13792722959366638No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA490.13516868500179305No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA480.13241014040991972No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA480.13241014040991972No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT480.13241014040991972No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT480.13241014040991972No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC470.12965159581804642No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT460.1268930512261731No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.1268930512261731No Hit
TGCGTATTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA460.1268930512261731No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT450.12413450663429976No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA440.1213759620424264No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC440.1213759620424264No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT430.11861741745055308No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT430.11861741745055308No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCAGGTAGTTAGACCAGCAC430.11861741745055308No Hit
TGCGTATTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA420.11585887285867975No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT420.11585887285867975No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA410.11310032826680642No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.11310032826680642No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC400.11034178367493311No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC400.11034178367493311No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.11034178367493311No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC390.10758323908305978No Hit
TGCGTATTCGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA390.10758323908305978No Hit
TCATGCCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT380.10482469449118645No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT380.10482469449118645No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC370.10206614989931313No Hit
TGCGTATTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA370.10206614989931313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)