FastQCFastQC Report
Sun 21 May 2023
EGAF00007847402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39991
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA18144.536020604636043No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12923.2307269135555496No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10142.5355705033632567No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA9672.4180440599134805No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8492.1229776699757443No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7231.8079067790252807No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6821.7053837113350505No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6811.7028831487084592No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA6451.612862894151184No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6331.5828561426320922No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA6171.5428471406066366No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6091.5228426395939088No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5981.4953364507014077No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5881.470330824435498No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5841.460328573929134No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5641.4103173213973144No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5351.3378010052261757No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5111.2777875021879923No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4291.0727413668075316No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4241.0602385536745769No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4081.020229551649121No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3970.9927233627566203No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3930.9827211122502563No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3720.9302092970918456No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3510.877697481933435No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3470.8676952314270711No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3470.8676952314270711No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3470.8676952314270711No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3410.8526918556675253No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3290.8226851041484333No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3190.7976794778825236No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3090.7726738516166137No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2900.7251631617113851No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.7001575354454752No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.6651496586732014No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.6651496586732014No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2620.6551474081668375No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2500.6251406566477458No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2460.6151384061413818No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2340.58513165462229No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2290.5726288414893351No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2280.5701282788627441No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2200.5501237778500163No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2150.5376209647170613No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2100.5251181515841064No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2070.5176164637043335No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2000.5001125253181966No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1980.49511140006501464No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1940.4851091495586507No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1910.4776074616788777No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1860.4651046485459228No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1850.4626040859193319No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1840.4601035232927409No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1730.43259733440024006No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1720.43009677177364913No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1660.41509339601410317No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1630.4075917081343302No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1620.4050911455077392No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1600.4000900202545573No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1580.3950888950013753No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1550.3875872071216024No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1520.38008551924182943No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1510.37758495661523844No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1440.36008101822910155No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1430.35758045560251056No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1370.34257707984296465No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1350.3375759545897827No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1340.3350753919631917No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1250.3125703283238729No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1240.3100697656972819No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1220.3050686404440999No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1220.3050686404440999No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1200.30006751519091795No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1200.30006751519091795No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.29756695256432697No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1170.292565827311145No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.290065264684554No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1140.28506413943137204No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1060.2650596384186442No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1050.2625590757920532No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1050.2625590757920532No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.2550573879122803No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT1010.2525568252856893No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1000.2500562626590983No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT980.24505513740591633No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT970.24255457477932535No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT950.23755344952614338No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT940.23505288689955242No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT910.22755119901977944No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA870.21754894851341552No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT850.21254782326023355No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT820.20504613538046063No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC790.19754444750068764No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC780.19504388487409666No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT770.1925433222475057No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCC770.1925433222475057No Hit
TCATGCCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT740.18504163436773274No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA730.18254107174114176No Hit
TCATGCCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT710.1775399464879598No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC690.17253882123477782No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.17003825860818683No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA680.17003825860818683No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA670.16753769598159585No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG670.16753769598159585No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA660.1650371333550049No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA660.1650371333550049No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT650.1625365707284139No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA640.16003600810182292No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA640.16003600810182292No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT600.15003375759545898No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC590.147533194968868No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.147533194968868No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA560.14003150708909506No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC560.14003150708909506No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA560.14003150708909506No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACACT550.13753094446250408No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA550.13753094446250408No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA550.13753094446250408No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCC530.1325298192093221No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT520.13002925658273112No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT520.13002925658273112No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA510.12752869395614014No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA510.12752869395614014No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT510.12752869395614014No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA500.12502813132954915No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA500.12502813132954915No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT500.12502813132954915No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.12502813132954915No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT500.12502813132954915No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA490.12252756870295817No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA490.12252756870295817No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC490.12252756870295817No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT480.1200270060763672No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCC480.1200270060763672No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC470.11752644344977621No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCC470.11752644344977621No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT460.11502588082318523No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT460.11502588082318523No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.11502588082318523No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT460.11502588082318523No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT460.11502588082318523No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.11252531819659424No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT440.11002475557000325No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA440.11002475557000325No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT430.10752419294341228No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA430.10752419294341228No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT430.10752419294341228No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT410.10252306769023031No Hit
TCATGCCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT410.10252306769023031No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCC410.10252306769023031No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCC410.10252306769023031No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT410.10252306769023031No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT410.10252306769023031No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT400.10002250506363933No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT400.10002250506363933No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.10002250506363933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)