FastQCFastQC Report
Sun 21 May 2023
EGAF00007847407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63944
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA21783.4061053421744028No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16052.510008757662955No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA13692.1409358188414864No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA13322.083072688602527No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12101.892280745652446No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA10781.6858501188539974No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA10091.577943200300263No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9791.5310271487551608No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT9691.5153884649067935No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9621.5044413862129364No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9111.424684098586263No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT9041.413737019892406No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7951.243275365945202No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA7861.2292005504816714No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA7621.1916677092455898No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7131.11503815838859No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7051.102527211309896No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7011.0962717377705493No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6591.0305892656074065No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6340.9914925559864882No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6040.9445765044413862No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA6040.9445765044413862No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA6040.9445765044413862No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5790.9054797948204679No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5480.8569998748905292No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5450.852308269736019No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5360.8382334542724884No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5180.8100838233454273No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4930.7709871137245089No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4540.7099962467158764No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4410.6896659577129989No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4410.6896659577129989No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4330.677155010634305No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4310.6740272738646316No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4230.6615163267859377No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4150.6490053797072438No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4010.6271112223195296No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3880.6067809333166521No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3790.5927061178531214No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3710.5801951707744276No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3690.5770674340047541No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT3650.5708119604654073No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3560.5567371450018767No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3530.5520455398473665No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT3300.5160765669961216No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC3160.49418240960840737No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3130.48949080445389714No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3060.47854372576004No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3020.4722882522206931No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC3020.4722882522206931No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3010.4707243838358564No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2940.45977730514199927No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2870.44883022644814213No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2860.4472663580633054No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT2850.4457024896784687No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2820.4410108845239584No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT2800.43788314775428494No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2680.4191167271362442No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2650.41442512198173403No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2620.4097335168272238No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT2600.4066057800575504No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2570.40191417490304016No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2560.40035030651820347No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2480.38783935943950953No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2470.38627549105467285No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2430.38002001751532594No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2400.3753284123608157No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA2390.37376454397597897No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2370.3706368072063055No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC2370.3706368072063055No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2350.36750907043663206No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2310.36125359689728515No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2280.3565619917427749No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2170.33935943950957087No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2160.3377955711247342No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2090.326848492430877No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2090.326848492430877No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC2070.32372075566120356No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2030.31746528212185665No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2020.3159014137370199No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2010.31433754535218317No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT2010.31433754535218317No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1950.30495433504316277No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1950.30495433504316277No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1800.28149630927061176No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1660.25960215188289754No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT1650.2580382834980608No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1640.25647441511322405No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1610.2517828099587139No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1590.24865507318904043No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1590.24865507318904043No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1550.2423995996496935No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1550.2423995996496935No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1540.24083573126485674No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT1530.23927186288002No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1520.23770799449518326No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1510.23614412611034655No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1490.23301638934067312No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1480.23145252095583638No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1460.2283247841861629No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1460.2283247841861629No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1440.22519704741648944No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT1410.2205054422619792No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.21737770549230578No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT1360.21268610033779559No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1300.2033028900287752No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT1300.2033028900287752No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1280.20017515325910173No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1270.198611284874265No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1250.1954835481045915No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1240.19391967971975477No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1240.19391967971975477No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1230.19235581133491805No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1230.19235581133491805No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1230.19235581133491805No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1210.1892280745652446No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1180.1845364694107344No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.18297260102589766No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1160.18140873264106094No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA1140.17828099587138746No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1130.17671712748655075No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1110.17358939071687726No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1080.1688977855623671No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1060.1657700487926936No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1040.16264231202302012No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.1563868384836732No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT990.15482297009883647No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.15325910171399976No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT980.15325910171399976No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT970.15169523332916302No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT970.15169523332916302No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC950.14856749655948956No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC930.1454397597898161No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT930.1454397597898161No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT920.14387589140497936No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT900.14074815463530588No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA900.14074815463530588No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT880.13762041786563242No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA880.13762041786563242No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT880.13762041786563242No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT880.13762041786563242No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA870.1360565494807957No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT860.13449268109595897No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT860.13449268109595897No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT840.1313649443262855No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC830.12980107594144877No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT820.12823720755661203No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA790.12354560240210184No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT790.12354560240210184No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA770.12041786563242837No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC760.11885399724759163No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCT750.1172901288627549No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC750.1172901288627549No Hit
TAGCATCGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA750.1172901288627549No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.1172901288627549No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.1172901288627549No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT740.11572626047791819No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT720.11259852370824472No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT720.11259852370824472No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT720.11259852370824472No Hit
TAGCATCGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA700.10947078693857124No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT700.10947078693857124No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC680.10634305016889779No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA680.10634305016889779No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC680.10634305016889779No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.10477918178406105No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.10477918178406105No Hit
TAGCATCGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA670.10477918178406105No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT660.10321531339922431No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA660.10321531339922431No Hit
TAGCATCGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA650.1016514450143876No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC640.10008757662955087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)