FastQCFastQC Report
Sun 21 May 2023
EGAF00007847424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41590
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA22305.361865833132964No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA13193.17143544121183No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA10002.40442414041837No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9662.322673719644145No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8732.099062274585237No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7351.7672517432075017No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT7101.7071411396970426No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6381.5340226015869198No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6141.476316422216879No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5931.4258235152680934No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5791.3921615773022362No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5751.3825438807405626No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5741.3801394566001444No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5431.3056023082471748No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5161.240682856455879No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA4941.1877855253666747No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4841.163741283962491No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4671.1228660735753788No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4030.968982928588603No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3960.9521519596056744No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3920.9425342630440009No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3830.9208944457802357No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3820.9184900216398173No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3720.8944457802356335No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3650.8776148112527049No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3530.8487617215676846No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3440.8271219043039192No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3420.8223130560230824No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.8030776628997356No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3120.7501803318105313No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3100.7453714835296946No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA3050.7333493628276028No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2930.7044962731425823No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2890.6948785765809089No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2830.6804520317383986No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2750.6612166386150518No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2710.6515989420533782No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2650.637172397210868No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2640.6347679730704496No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2630.6323635489300312No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2530.6083193075258475No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2490.5987016109641741No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2420.5818706419812455No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2420.5818706419812455No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2320.5578264005770618No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2270.5458042798749699No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2180.5241644626112046No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2160.5193556143303679No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2150.5169511901899495No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2090.5025246453474393No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1970.47367155566241886No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1850.4448184659773984No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1790.43039192113488817No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1790.43039192113488817No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1760.4231786487136331No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1710.4111565280115412No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1710.4111565280115412No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1680.4039432555902861No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1650.396729983169031No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1650.396729983169031No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1600.38470786246693917No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1600.38470786246693917No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1480.35585477278191874No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1430.3438326520798269No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1420.34142822793940847No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1390.3342149555181534No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1350.3245972589564799No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1340.32219283481606154No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1330.31978841067564323No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1320.3173839865352248No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1290.31017071411396974No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1270.30536186583313296No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1260.30295744169271455No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1250.30055301755229624No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1240.2981485934118779No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.286126472709786No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.2789132002885309No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1140.2741043520076941No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1100.2644866554460207No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.25967780716518396No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1070.25727338302476555No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1060.2548689588843472No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1050.2524645347439288No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1040.25006011060351047No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1030.2476556864630921No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.2476556864630921No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1020.24525126232267372No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC990.2380379899014186No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC980.23563356576100022No Hit
ATCGTGAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA980.23563356576100022No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA930.22361144505890837No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC930.22361144505890837No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT920.22120702091849004No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC920.22120702091849004No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT910.21880259677807162No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT910.21880259677807162No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA890.2139937484972349No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT890.2139937484972349No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA880.21158932435681654No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA870.20918490021639818No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT860.2067804760759798No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA860.2067804760759798No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT840.20197162779514305No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT830.1995672036547247No Hit
TCATGCCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT810.19475835537388794No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.18754508295263284No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT760.18273623467179612No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT720.17311853811012262No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC710.17071411396970423No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT710.17071411396970423No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA700.1683096898292859No Hit
TCATGCCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT690.16590526568886751No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG670.16109641740803077No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT660.1586919932676124No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC660.1586919932676124No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT640.15388314498677566No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC640.15388314498677566No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA640.15388314498677566No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.15147872084635727No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT620.14907429670593894No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT620.14907429670593894No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA620.14907429670593894No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC610.14666987256552055No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT610.14666987256552055No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA600.1442654484251022No Hit
TCATGCCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT590.1418610242846838No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA580.13945660014426545No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC580.13945660014426545No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.13945660014426545No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.13705217600384706No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA550.13224332772301034No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT540.12983890358259198No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA540.12983890358259198No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.1274344794421736No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT530.1274344794421736No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA530.1274344794421736No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG520.12503005530175523No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC520.12503005530175523No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA510.12262563116133686No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA510.12262563116133686No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.12022120702091849No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.12022120702091849No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC490.11781678288050011No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT480.11541235874008177No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA480.11541235874008177No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC480.11541235874008177No Hit
ATCGTGAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA480.11541235874008177No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.11541235874008177No Hit
ATCGTGAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA470.11300793459966339No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.11300793459966339No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT460.11060351045924502No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC460.11060351045924502No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC460.11060351045924502No Hit
ATCGTGAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG460.11060351045924502No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT450.10819908631882665No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC450.10819908631882665No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT450.10819908631882665No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT450.10819908631882665No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC440.10579466217840827No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC440.10579466217840827No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT430.1033902380379899No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT420.10098581389757152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)