FastQCFastQC Report
Sun 21 May 2023
EGAF00007847452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34654
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14674.233277543717897No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11983.4570323772147513No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA7382.129624285796733No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7202.0776822300455935No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6711.936284411611935No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6251.8035436024701332No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA6111.7631442257748025No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5391.555376002770243No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5231.5092052865470076No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4921.419749523864489No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4731.3649217983493969No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4391.2668090263750216No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4221.217752640387834No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3731.0763548219541756No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3731.0763548219541756No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3711.070583482426271No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3671.0590408033704624No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3460.9984417383274657No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3290.9493853523402781No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3180.9176429849368037No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3170.9147573151728516No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3090.8916719570612339No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3070.8859006175333296No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2980.8599295896577595No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2920.8426155710740463No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2910.8397299013100941No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2830.8166445431984763No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC2600.7502741386275755No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2600.7502741386275755No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2510.7243031107520056No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2500.7214174409880534No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2440.7041034224043401No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2300.6637040457090091No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2240.6463900271252957No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.6435043573613436No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2200.634847348069487No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2200.634847348069487No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2130.6146476597218214No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2130.6146476597218214No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC2130.6146476597218214No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2100.6059906504299648No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC2090.6031049806660126No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2020.5829052923183471No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2010.5800196225543949No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1990.5742482830264904No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1970.568476943498586No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1900.5482772551509205No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1830.528077566803255No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1820.5251918970393028No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1800.5194205575113984No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1780.5136492179834939No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1760.5078778784555895No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1750.5049922086916373No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1550.4472788134125931No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1510.4357361343567842No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1460.42130778553702314No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1460.42130778553702314No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1390.40110809718935764No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1390.40110809718935764No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1380.3982224274254055No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1370.3953367576614532No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1370.3953367576614532No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1330.38379407860564435No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1300.37513706931378776No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1300.37513706931378776No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1260.36359439025797885No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1250.3607087204940267No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC1250.3607087204940267No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1240.3578230507300744No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1240.3578230507300744No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1190.34339470191031335No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1180.3405090321463612No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1100.3174236740347435No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1090.31453800427079126No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1090.31453800427079126No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1090.31453800427079126No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1070.30876666474288683No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1050.3029953252149824No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1030.297223985687078No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1030.297223985687078No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1020.29433831592312576No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.29145264615917355No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1000.28856697639522133No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT980.2827956368673169No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.2827956368673169No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA970.2799099671033647No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA940.27125295781150804No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA930.26836728804755583No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC920.2654816182836036No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG910.2625959485196514No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC900.2597102787556992No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC890.25682460899174697No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT870.25105326946384254No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT860.24816759969989036No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG860.24816759969989036No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA850.24528192993593811No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.23085358111617707No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT790.22796791135222486No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA760.21931090206036818No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT750.216425232296416No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA750.216425232296416No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA750.216425232296416No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT740.2135395625324638No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG740.2135395625324638No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA730.21065389276851157No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT720.20776822300455935No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.2048825532406071No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC700.20199688347665493No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT690.19911121371270274No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC690.19911121371270274No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA690.19911121371270274No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC680.1962255439487505No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA670.1933398741847983No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA660.1904542044208461No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT660.1904542044208461No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT650.18756853465689388No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT640.18468286489294164No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC640.18468286489294164No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC620.1789115253650372No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC590.1702545160731806No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT580.16736884630922838No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC580.16736884630922838No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.16448317654527617No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA560.16159750678132392No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA560.16159750678132392No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC560.16159750678132392No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC540.15582616725341952No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC530.1529404974894673No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.1500548277255151No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT520.1500548277255151No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA500.14428348819761067No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA500.14428348819761067No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA500.14428348819761067No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.14139781843365845No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA490.14139781843365845No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.13562647890575402No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG470.13562647890575402No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.13562647890575402No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT470.13562647890575402No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT460.1327408091418018No Hit
TCTATGTCGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA460.1327408091418018No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC460.1327408091418018No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT450.1298551393778496No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT430.12408379984994518No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT430.12408379984994518No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC420.12119813008599296No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT420.12119813008599296No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC410.11831246032204075No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT410.11831246032204075No Hit
TCTATGTCGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA410.11831246032204075No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT400.11542679055808854No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT400.11542679055808854No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.11542679055808854No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA400.11542679055808854No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT390.1125411207941363No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACC390.1125411207941363No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT380.10965545103018409No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT370.1067697812662319No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT370.1067697812662319No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA370.1067697812662319No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT370.1067697812662319No Hit
TCTATGTCGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA360.10388411150227968No Hit
TCTATGTCGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA350.10099844173832746No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT350.10099844173832746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)