FastQCFastQC Report
Sun 21 May 2023
EGAF00007847456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44311
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11792.660738868452529No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10792.4350612714675814No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9772.2048701225429355No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9452.132653291507752No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6991.5774864029247815No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6731.5188102277086954No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6251.4104849811559206No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6201.3992011013066732No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6161.3901739974272753No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6101.3766333416081786No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6061.3676062377287805No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5801.3089300625126943No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5681.2818487508745007No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5661.2773351989348016No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5571.2570242152061566No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5341.2051183678996185No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5281.1915777120805218No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5241.182550608201124No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5201.173523504321726No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5181.169009952382027No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5151.1622396244724786No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4941.1148473291056398No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4801.0832524655277471No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4801.0832524655277471No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4741.0697118097086502No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4711.0629414817991019No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4581.0336033941910587No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4571.0313466182212092No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4430.9997517546433166No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4410.9952382027036176No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4310.9726704430051228No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4210.9501026833066282No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3940.8891697321206924No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3860.8711155243618965No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3590.8101825731759608No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3410.7695606057186704No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3400.7673038297488208No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3360.7582767258694229No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3270.7379657421407777No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3000.6770327909548419No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2680.6048159599196589No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2580.582248200221164No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2530.5709643203719167No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2520.5687075444020673No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2360.5325991288844757No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.5077745932161314No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2230.5032610412764326No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2200.4964907133668841No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2190.4942339373970346No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2180.4919771614271851No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2170.48972038545733565No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2170.48972038545733565No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2000.45135519396989465No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1990.44909841800004513No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1950.44007131412064726No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1910.4310442102412494No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1890.4265306583015504No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1870.4220171063618515No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1860.41976033039200195No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1840.41524677845230307No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1820.4107332265126041No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1780.4017061226332062No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1760.3971925706935072No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1760.3971925706935072No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1720.38816546681410935No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1680.3791383629347114No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.3791383629347114No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1640.37011125905531356No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1640.37011125905531356No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1630.3678544830854641No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.36334093114576516No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1580.35657060323621675No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.3498002753266683No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1500.33851639547742096No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1500.33851639547742096No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1490.3362596195075715No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1480.334002843537722No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1440.32497573965832416No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1430.32271896368847464No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1380.3114350838392273No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1380.3114350838392273No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1340.30240797995982943No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1320.29789442802013044No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1290.291124100110582No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1240.27984022026133465No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1210.27306989235178625No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1190.26855634041208726No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1170.2640427884723883No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1170.2640427884723883No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1160.26178601250253886No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1140.2572724605628399No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1050.2369614768341947No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1030.2324479248944957No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.2324479248944957No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1010.22793437295479677No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1010.22793437295479677No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1000.22567759698494733No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.22567759698494733No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC990.22342082101509783No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT990.22342082101509783No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA960.2166504931055494No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT950.2143937171356999No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT930.20988016519600097No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA890.2008530613166031No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA890.2008530613166031No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA890.2008530613166031No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT880.1985962853467536No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT860.19408273340705468No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA860.19408273340705468No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA830.18731240549750625No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA800.18054207758795784No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA790.17828530161810838No Hit
TCATGCCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT780.17602852564825888No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC760.17151497370855995No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.17151497370855995No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA750.16925819773871048No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC750.16925819773871048No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC710.16023109385931258No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT710.16023109385931258No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.16023109385931258No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA700.15797431788946312No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC690.15571754191961365No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC680.15346076594976418No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA660.14894721401006522No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.14669043804021575No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT650.14669043804021575No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC640.1444336620703663No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.1444336620703663No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT640.1444336620703663No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA640.1444336620703663No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC620.13992011013066732No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA610.13766333416081786No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.13766333416081786No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT600.1354065581909684No Hit
TCATGCCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT590.13314978222111892No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT590.13314978222111892No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT590.13314978222111892No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA590.13314978222111892No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT580.13089300625126943No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA580.13089300625126943No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.12863623028141996No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA570.12863623028141996No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC560.1263794543115705No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC560.1263794543115705No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA540.12186590237187153No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC540.12186590237187153No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA540.12186590237187153No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAA530.11960912640202206No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA520.1173523504321726No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA510.11509557446232313No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT510.11509557446232313No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC510.11509557446232313No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT510.11509557446232313No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.11509557446232313No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC490.11058202252262417No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA490.11058202252262417No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA470.10606847058292523No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC470.10606847058292523No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT460.10381169461307577No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT460.10381169461307577No Hit
TCATGCCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT460.10381169461307577No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA460.10381169461307577No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT450.10155491864322627No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT450.10155491864322627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)