FastQCFastQC Report
Sun 21 May 2023
EGAF00007847457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44311
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11562.608833021145991No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10842.446345151316829No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9412.123626187628354No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9142.0626932364424184No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7501.692581977387105No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7471.6858116494775564No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6291.4195120850353187No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6111.378890117578028No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6041.3630926857890817No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5911.3337545981810386No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5771.302159734603146No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5751.2976461826634471No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5641.2728216469951028No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5631.2705648710252533No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5501.2412267834172102No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5421.2231725756584144No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5411.220915799688565No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5411.220915799688565No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5021.1329015368644355No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4931.1125905531357903No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4891.1035634492563924No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4751.0719685856784997No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4701.0606847058292523No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4531.0223195143418113No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4491.0132924104624135No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4420.997494978673467No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4310.9726704430051228No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4210.9501026833066282No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4090.9230213716684345No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4040.9117374918191871No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3960.8936832840603913No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3920.8846561801809933No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3870.8733723003317462No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3440.7763309336282187No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3390.7650470537789713No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3360.7582767258694229No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3220.7266818622915303No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3210.7244250863216809No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3100.6996005506533366No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3010.6792895669246914No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3000.6770327909548419No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2980.672519239015143No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2760.6228701676784546No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2700.6093295118593577No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2640.5957888560402609No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2560.5777346482814651No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2510.5664507684322178No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2470.5574236645528199No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2390.5393694567940241No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2380.5371126808241745No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2160.4874636094874861No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2140.48295005754778725No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2120.47843650560808826No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2060.4648958497889914No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2060.4648958497889914No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2030.458125521879443No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2010.45361196993974406No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1960.4423280900904967No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.4423280900904967No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1880.42427388233170094No Hit
TCATGCCTTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1880.42427388233170094No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1780.4017061226332062No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1780.4017061226332062No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1680.3791383629347114No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1680.3791383629347114No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1670.376881586964862No Hit
TCATGCCTTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1620.3655977071156146No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1600.3610841551759157No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1590.3588273792060662No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1570.3543138272663673No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1540.34754349935681883No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1530.34528672338696936No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1490.3362596195075715No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1480.334002843537722No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.334002843537722No Hit
TCATGCCTTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1470.33174606756787256No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1430.32271896368847464No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1420.32046218771862517No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1390.31369185980907677No Hit
TCATGCCTTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1360.30692153189952837No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1320.29789442802013044No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1260.28435377220103364No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1220.2753266683216357No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1190.26855634041208726No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1190.26855634041208726No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1190.26855634041208726No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1180.26629956444223785No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1170.2640427884723883No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1160.26178601250253886No Hit
TCATGCCTTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1120.252758908623141No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1100.24824535668344205No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.24598858071359256No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.24373180474374306No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1070.24147502877389362No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.2347047008643452No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1000.22567759698494733No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC990.22342082101509783No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA980.22116404504524834No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC980.22116404504524834No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT980.22116404504524834No Hit
TCATGCCTTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT980.22116404504524834No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA980.22116404504524834No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT960.2166504931055494No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA950.2143937171356999No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT930.20988016519600097No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA900.20310983728645254No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA900.20310983728645254No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT900.20310983728645254No Hit
TCATGCCTTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT890.2008530613166031No Hit
TCATGCCTTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT880.1985962853467536No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA870.19633950937690417No Hit
TCATGCCTTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.1918259574372052No Hit
TCATGCCTTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT850.1918259574372052No Hit
TCATGCCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT840.1895691814673557No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA800.18054207758795784No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC800.18054207758795784No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT800.18054207758795784No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA790.17828530161810838No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA780.17602852564825888No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.16474464579901155No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT720.16248786982916208No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT710.16023109385931258No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC700.15797431788946312No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA690.15571754191961365No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA680.15346076594976418No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA670.15120398997991472No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC670.15120398997991472No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.15120398997991472No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA660.14894721401006522No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT660.14894721401006522No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC650.14669043804021575No Hit
TCATGCCTTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT650.14669043804021575No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA640.1444336620703663No Hit
TCATGCCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT630.14217688610051682No Hit
TCATGCCTTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT630.14217688610051682No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT600.1354065581909684No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.13314978222111892No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAA580.13089300625126943No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.12863623028141996No Hit
ACTCTTCTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA560.1263794543115705No Hit
ACTCTTCTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA560.1263794543115705No Hit
TCATGCCTTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC560.1263794543115705No Hit
TCATGCCTTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT550.12412267834172103No Hit
TCATGCCTTCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC550.12412267834172103No Hit
TCATGCCTTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.12186590237187153No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA530.11960912640202206No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA530.11960912640202206No Hit
TCATGCCTTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.11960912640202206No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC510.11509557446232313No Hit
TCATGCCTTCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC500.11283879849247366No Hit
ACTCTTCTTGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA500.11283879849247366No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC490.11058202252262417No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA490.11058202252262417No Hit
TCATGCCTTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.11058202252262417No Hit
TCATGCCTTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.1083252465527747No Hit
TCATGCCTTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT460.10381169461307577No Hit
ACTCTTCTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA460.10381169461307577No Hit
TCATGCCTTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT450.10155491864322627No Hit
TCATGCCTTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT450.10155491864322627No Hit
TCATGCCTTCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT450.10155491864322627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)