FastQCFastQC Report
Sun 21 May 2023
EGAF00007847480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36199
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15464.2708362109450535No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10622.9337827011795903No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA10182.812232382110003No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8622.3812812508632835No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8242.276305975303185No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7462.0608304096798253No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5661.5635791043951492No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5341.4751788723445398No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5311.4668913505897951No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5261.4530788143318878No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5241.4475537998287245No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5161.4254537418160722No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5121.4144037128097462No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4981.3757286112876046No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4501.243128263211691No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4461.2320782342053649No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4151.1464405094063372No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4131.1409154949031741No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA4111.135390480400011No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4041.1160529296389403No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3621.0000276250725157No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3621.0000276250725157No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3100.8563772479902759No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3050.8425647117323684No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2860.7900770739523191No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2820.779027044945993No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2760.7624520014365038No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2700.7458769579270146No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.7348269289206885No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2650.7320644216691069No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2630.7265394071659439No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2580.7127268709080361No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2570.7099643636564545No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2560.7072018564048731No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2380.6574767258764054No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA2240.6188016243542639No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2230.6160391171026824No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2150.5939390590900301No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2090.577364015580541No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2080.5746015083289593No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2060.5690764938257963No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1990.5497389430647256No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1960.5414514213099809No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1950.5386889140583994No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1920.5304013923036548No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1880.5193513632973287No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1860.5138263487941656No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1850.511063841542584No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1800.49725130528467637No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1790.49448879803309487No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1790.49448879803309487No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1770.4889637835299318No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1760.4862012762783502No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1730.4779137545236056No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1710.47238874002044257No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1630.45028868200779026No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1610.4447636675046272No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1560.4309511312467196No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1560.4309511312467196No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1560.4309511312467196No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1510.41713859498881184No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1510.41713859498881184No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1470.4060885659824857No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1460.4033260587309042No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1450.40056355147932265No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1420.392276029724578No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1410.3895135224729965No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1400.386751015221415No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1400.386751015221415No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1400.386751015221415No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1320.36465095720876267No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1290.35636343545401805No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1290.35636343545401805No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1290.35636343545401805No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1280.35360092820243655No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1270.350838420950855No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1250.34531340644769193No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1240.34255089919611037No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1190.3287383629382027No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1190.3287383629382027No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1170.3232133484350396No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.3121633194287135No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1090.3011132904223873No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1070.29558827591922426No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1060.29282576866764276No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1040.28730075416447964No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1010.2790132324097351No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT960.2652006961518274No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT950.2624381889002459No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA930.2569131743970828No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA920.2541506671455013No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC920.2541506671455013No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG890.24586314539075665No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA870.24033813088759357No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC860.23757562363601203No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT860.23757562363601203No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA820.22652559462968588No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC800.22100058012652282No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA790.21823807287494132No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT770.2127130583717782No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA760.20995055112019667No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT760.20995055112019667No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.20995055112019667No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC740.20442553661703364No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT700.1933755076107075No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT700.1933755076107075No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC700.1933755076107075No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA690.19061300035912596No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT680.1878504931075444No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA670.18508798585596287No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA670.18508798585596287No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT660.18232547860438134No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT650.1795629713527998No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT650.1795629713527998No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT630.17403795684963674No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG630.17403795684963674No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT620.17127544959805518No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT600.16575043509489212No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.16022542059172906No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.16022542059172906No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC570.1574629133401475No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT560.15470040608856597No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA540.1491753915854029No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC540.1491753915854029No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.14641288433382138No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT510.1408878698306583No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA490.13536285532749523No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC490.13536285532749523No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT490.13536285532749523No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC490.13536285532749523No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT490.13536285532749523No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT490.13536285532749523No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC480.1326003480759137No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC460.12707533357275064No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCCGGCGTTA460.12707533357275064No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.12431282632116909No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.12431282632116909No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT440.12155031906958755No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT430.11878781181800602No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT430.11878781181800602No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA430.11878781181800602No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA420.11602530456642449No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT420.11602530456642449No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC420.11602530456642449No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT420.11602530456642449No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT410.11326279731484294No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG410.11326279731484294No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.11050029006326141No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT390.1077377828116799No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT390.1077377828116799No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC390.1077377828116799No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT390.1077377828116799No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT390.1077377828116799No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA390.1077377828116799No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC380.10497527556009834No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT380.10497527556009834No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA370.10221276830851682No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC370.10221276830851682No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT370.10221276830851682No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA370.10221276830851682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)