FastQCFastQC Report
Sun 21 May 2023
EGAF00007847481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36199
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15224.204536036907097No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA10522.9061576286637756No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10072.781844802342606No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7742.1381806127241085No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7382.0387303516671733No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6431.7762921627669273No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6081.6796044089615734No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5961.6464543219425951No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5771.593966684162546No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5191.4337412635708169No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4921.3591535677781155No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4771.3177159590043925No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4711.3011409154949032No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4391.212740683444294No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4171.1519655239095004No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4151.1464405094063372No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4041.1160529296389403No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3931.0856653498715434No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3781.0442277410978205No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3580.9889775960661897No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA3560.9834525815630266No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3520.9724025525567004No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3440.9503024945440481No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3290.9088648857703252No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3170.8757147987513467No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3140.8674272769966022No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3020.8342771899776237No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2880.7956020884554821No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2820.779027044945993No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2780.7679770159396668No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2750.7596894941849223No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2710.748639465178596No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2650.7320644216691069No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2500.6906268128953839No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2500.6906268128953839No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2430.6712892621343132No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2310.6381391751153347No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2240.6188016243542639No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC2210.6105141025995193No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2170.5994640735931932No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2130.5884140445868671No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA2100.5801265228321225No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2050.5663139865742147No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2010.5552639575678886No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2000.5525014503163072No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2000.5525014503163072No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1990.5497389430647256No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1940.5359264068068179No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1920.5304013923036548No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1880.5193513632973287No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1870.5165888560457471No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1770.4889637835299318No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1730.4779137545236056No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1730.4779137545236056No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1720.47515124727202407No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1710.47238874002044257No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1660.45857620376253494No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1640.45305118925937177No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1630.45028868200779026No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1570.4337136384983011No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1570.4337136384983011No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1520.41990110224039334No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1510.41713859498881184No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1480.4088510732340673No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1440.39780104422774104No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1430.39503853697615954No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1420.392276029724578No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1400.386751015221415No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC1360.3757009862150888No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1330.36741346446034423No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1300.3591259427055996No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1290.35636343545401805No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1290.35636343545401805No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.35360092820243655No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1270.350838420950855No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.3370258846929473No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1130.3121633194287135No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC1110.30663830492555044No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1100.30387579767396894No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1060.29282576866764276No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1050.29006326141606126No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1040.28730075416447964No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.28730075416447964No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1030.28453824691289814No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1020.2817757396613166No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1020.2817757396613166No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1020.2817757396613166No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1000.2762507251581536No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1000.2762507251581536No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC990.273488217906572No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG970.26796320340340896No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG970.26796320340340896No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA940.25967568164866434No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA930.2569131743970828No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG910.2513881598939197No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT840.23205060913284897No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA820.22652559462968588No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA820.22652559462968588No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT810.22376308737810435No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT810.22376308737810435No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG800.22100058012652282No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC800.22100058012652282No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT790.21823807287494132No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA780.2154755656233598No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT750.20718804386861517No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT740.20442553661703364No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA730.2016630293654521No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA730.2016630293654521No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA730.2016630293654521No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.1933755076107075No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA690.19061300035912596No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC680.1878504931075444No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC680.1878504931075444No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG640.17680046410121827No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC640.17680046410121827No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT620.17127544959805518No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT620.17127544959805518No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT610.16851294234647365No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC600.16575043509489212No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT580.16022542059172906No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA550.15193789883698447No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT550.15193789883698447No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT540.1491753915854029No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA530.14641288433382138No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT520.14365037708223982No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA500.1381253625790768No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT500.1381253625790768No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT500.1381253625790768No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.1381253625790768No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA490.13536285532749523No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCCGGCGTTA480.1326003480759137No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.1326003480759137No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT470.12983784082433217No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT470.12983784082433217No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.12431282632116909No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA450.12431282632116909No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT450.12431282632116909No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA440.12155031906958755No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTCGATACCCGCACC430.11878781181800602No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT420.11602530456642449No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT420.11602530456642449No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCCGGCGTTA410.11326279731484294No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT400.11050029006326141No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT400.11050029006326141No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT400.11050029006326141No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC400.11050029006326141No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT400.11050029006326141No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT400.11050029006326141No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG400.11050029006326141No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT400.11050029006326141No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC390.1077377828116799No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT390.1077377828116799No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT390.1077377828116799No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC380.10497527556009834No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT370.10221276830851682No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT370.10221276830851682No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA370.10221276830851682No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT370.10221276830851682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)