FastQCFastQC Report
Sun 21 May 2023
EGAF00007847496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48047
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA20984.366557745540824No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA19974.156346910316981No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA11312.353945095427394No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11222.335213436843091No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA10422.1687098049826212No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10192.120840010822736No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8541.777426270110517No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8111.687930567985514No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7901.6442233646221407No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7731.6088413428517907No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA7631.5880283888692324No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6691.3923866214331801No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6501.3528420088663184No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6451.342435531875039No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6261.3028909193081775No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6101.2695901929360833No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5731.192582263200616No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5631.1717693092180574No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5561.1572002414302662No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5201.0822736070930548No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4871.0135908589506109No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4710.9802901325785169No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4630.9636397693924699No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4460.92825774762212No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4420.9199325660290966No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4380.9116073844360729No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4120.8574937040814202No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4020.8366807500988616No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3950.8221116823110703No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3810.7929735467354883No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3770.7846483651424646No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3450.7180469123982767No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3440.7159656170000208No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3430.713884321601765No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3320.6909900722209503No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3220.6701771182383915No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2820.5869253023081565No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2660.5536245759360626No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2590.5390555081482715No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2540.5286490311569921No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2500.5203238495639686No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2450.5099173725726892No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2430.5057547817761775No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.49534830478489816No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2350.48910441859013043No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2260.4703727600058276No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2260.4703727600058276No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2240.4662101692093159No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2200.45788498761629237No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2190.45580369221803646No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2180.45372239681978066No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2180.45372239681978066No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2170.45164110142152475No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2020.4204216704476867No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2010.4183403750494308No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1960.4079338980581514No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1950.40585260265989553No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1920.3996087164651279No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1810.3767144670843133No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1810.3767144670843133No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1770.36838928549128974No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1770.36838928549128974No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1750.36422669469477803No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1700.3538202177034987No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1620.33716985451745163No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1580.32884467292442815No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1550.3226007867296605No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1540.32051949133140467No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA1530.3184381959331488No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1520.31635690053489296No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1440.2997065373488459No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1420.2955439465523342No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1420.2955439465523342No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1400.2913813557558224No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1320.27473099256977546No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1300.2705684017732637No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1300.2705684017732637No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1290.2684871063750078No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1280.2664058109767519No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1260.26224322018024016No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1250.2601619247819843No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1250.2601619247819843No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1230.25599933398547253No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1230.25599933398547253No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.2539180385872167No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1190.24767415239244908No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1190.24767415239244908No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA1160.24143026619768143No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1160.24143026619768143No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.2351863800029138No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1130.2351863800029138No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1100.22894249380814619No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.22686119840989033No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.22686119840989033No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1080.22477990301163442No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1080.22477990301163442No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1060.22061731221512268No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1050.21853601681686682No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1030.21437342602035506No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.20812953982558743No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT980.2039669490290757No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT970.2018856536308198No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC970.2018856536308198No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA970.2018856536308198No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT940.19564176743605222No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT900.18731658584302868No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA890.18523529044477283No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC890.18523529044477283No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG890.18523529044477283No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA890.18523529044477283No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC870.1810726996482611No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA840.17482881345349346No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.17274751805523758No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC820.1706662226569817No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA810.16858492725872581No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.16858492725872581No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC810.16858492725872581No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT800.16650363186046996No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA790.16442233646221407No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC790.16442233646221407No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT790.16442233646221407No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT770.16025974566570234No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC770.16025974566570234No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT760.15817845026744648No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC750.1560971548691906No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA740.1540158594709347No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC730.15193456407267883No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG710.1477719732761671No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC710.1477719732761671No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA700.1456906778779112No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA680.14152808708139947No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT680.14152808708139947No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA680.14152808708139947No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT670.13944679168314358No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT650.13528420088663184No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT650.13528420088663184No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA640.13320290548837596No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACT620.12904031469186422No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT610.12695901929360834No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC610.12695901929360834No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT610.12695901929360834No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC600.12487772389535247No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.11863383770058485No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.11863383770058485No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA570.11863383770058485No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT560.11655254230232898No Hit
TCTCTAAGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG560.11655254230232898No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.11655254230232898No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT550.11447124690407309No Hit
TCTCTAAGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA550.11447124690407309No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT550.11447124690407309No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC540.11238995150581721No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.11238995150581721No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.11238995150581721No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.11030865610756134No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT530.11030865610756134No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT520.10822736070930548No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT520.10822736070930548No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC510.1061460653110496No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC510.1061460653110496No Hit
TCTCTAAGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA500.10406476991279372No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT500.10406476991279372No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA500.10406476991279372No Hit
TCTCTAAGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA500.10406476991279372No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT500.10406476991279372No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACT490.10198347451453785No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT490.10198347451453785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)