FastQCFastQC Report
Sun 21 May 2023
EGAF00007847510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31788
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA18735.892160563734743No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11573.6397382660123316No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10613.337737511010444No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5931.8654838303762424No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5581.7553793884484712No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5481.723920976469108No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5441.7113376116773624No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5371.689316723291808No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5051.588649804957846No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5021.579212281364037No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4641.4596703158424562No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4211.3243991443311942No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4161.3086699383415124No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3721.1702529256323142No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3681.157669560840569No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3611.1356486724550146No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3601.1325028312570782No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3551.1167736252673965No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3391.0664401661004153No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3261.025544230527243No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3070.9657732477664528No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3020.9500440417767712No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2930.9217314709953442No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2820.8871272178180446No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2750.8651063294324902No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2730.8588146470366176No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2720.8556688058386812No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2700.8493771234428085No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2700.8493771234428085No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2680.843085441046936No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2670.8399395998489996No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2570.8084811878696363No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2570.8084811878696363No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2550.8021895054737638No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2520.7927519818799547No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2090.6574808103686925No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2070.6511891279728199No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2060.6480432867748835No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2010.6323140807852019No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1950.613439033597584No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1910.6008556688058387No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1910.6008556688058387No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1810.5693972568264755No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1770.5568138920347301No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1720.5410846860450484No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1690.5316471624512394No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1680.5285013212533031No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1680.5285013212533031No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1650.5190637976594942No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1630.5127721152636215No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1590.5001887504718762No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1590.5001887504718762No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1540.4844595444821945No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1500.4718761796904492No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1420.44670945010695856No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1380.4341260853152133No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1360.4278344029193406No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1340.421542720523468No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1260.39637599093997733No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1230.3869384673461684No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1200.3775009437523594No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.3554800553668051No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1110.3491883729709324No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1080.33975084937712347No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1080.33975084937712347No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1050.33031332578331446No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1040.3271674845853781No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC990.3114382785956965No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT970.3051465961998238No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT950.2988549138039512No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA920.28941739021014223No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT880.27683402541839686No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC880.27683402541839686No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA850.2673965018245879No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT830.2611048194287152No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA800.25166729583490627No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA790.24852145463696995No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA770.24222977224109726No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA750.2359380898452246No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC740.23279224864728829No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT720.22650056625141565No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT720.22650056625141565No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT720.22650056625141565No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT690.21706304265760665No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC690.21706304265760665No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA690.21706304265760665No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA680.2139172014596703No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT680.2139172014596703No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.210771360261734No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA660.20762551906379767No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA650.20447967786586135No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA650.20447967786586135No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT640.20133383666792498No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT640.20133383666792498No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC630.19818799546998866No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT630.19818799546998866No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT620.19504215427205235No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA620.19504215427205235No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA610.19189631307411603No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.19189631307411603No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC610.19189631307411603No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA610.19189631307411603No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT600.1887504718761797No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT600.1887504718761797No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC580.18245878948030703No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA580.18245878948030703No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT580.18245878948030703No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA570.1793129482823707No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.1793129482823707No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT550.17302126588649805No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC550.17302126588649805No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT530.1667295834906254No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT530.1667295834906254No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA520.16358374229268904No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT510.16043790109475273No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC500.1572920598968164No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC500.1572920598968164No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA490.15414621869888007No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT480.15100037750094375No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT470.14785453630300743No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.14785453630300743No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA470.14785453630300743No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT460.14470869510507112No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA460.14470869510507112No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT440.13841701270919843No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA440.13841701270919843No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC440.13841701270919843No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT440.13841701270919843No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC440.13841701270919843No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT440.13841701270919843No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA430.1352711715112621No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT430.1352711715112621No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA430.1352711715112621No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT420.13212533031332577No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA410.12897948911538945No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA410.12897948911538945No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.12897948911538945No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA400.12583364791745313No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA400.12583364791745313No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC400.12583364791745313No Hit
ATTAAGCCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA390.1226878067195168No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT390.1226878067195168No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT390.1226878067195168No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT380.11954196552158046No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT380.11954196552158046No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA370.11639612432364414No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT370.11639612432364414No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC370.11639612432364414No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTATGGGGCGAGCGTTG360.11325028312570783No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.11325028312570783No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT360.11325028312570783No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT350.11010444192777148No Hit
ATTAAGCCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA350.11010444192777148No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA340.10695860072983515No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT330.10381275953189883No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT330.10381275953189883No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC330.10381275953189883No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT330.10381275953189883No Hit
ATTAAGCCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA320.10066691833396249No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC320.10066691833396249No Hit
ATTAAGCCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA320.10066691833396249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)