FastQCFastQC Report
Sun 21 May 2023
EGAF00007847519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51291
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA14292.78606383186134No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA14032.7353726774677822No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA9441.8404788364430407No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9321.817082919030629No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA8791.7137509504591448No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8661.6884053732623656No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6731.3121210348794135No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6431.2536312413483848No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6351.2380339630734438No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6171.2029400869548263No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6161.2009904271704588No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6091.1873428086798854No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5891.1483496129925328No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5821.1347019945019594No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5781.126903355364489No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5491.0703632216178276No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5301.0333196857148428No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5080.990427170458755No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4740.9241387377902557No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4670.9104911192996822No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4620.900742820377844No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4580.8929441812403736No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC4550.8870952018872706No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4490.8753972431810648No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4020.7837632333157865No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC3980.7759645941783159No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC3970.7740149343939483No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3960.7720652746095806No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3870.754518336550272No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3790.7389210582753309No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3670.7155251408629194No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC3540.6901795636661403No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3530.6882299038817726No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3530.6882299038817726No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3420.6667836462537287No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3380.6589850071162582No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3380.6589850071162582No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3360.6550856875475229No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3300.6433877288413172No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3270.6375387494882143No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3070.5985455538008617No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC3060.5965958940164942No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC3030.5907469146633912No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2820.549804059191671No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2800.5459047396229358No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2800.5459047396229358No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2730.5322571211323625No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2660.5186095026417891No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2630.5127605232886862No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2610.5088612037199508No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2590.5049618841512157No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2580.503012224366848No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2520.49131426566064224No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2510.4893646058762746No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2490.4854652863075393No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC2410.4698680080325983No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2390.465968688463863No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2330.45427072975765725No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2120.4133278742859371No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2110.4113782145015695No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2110.4113782145015695No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA2090.4074788949328342No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC2060.4016299155797314No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC2050.3996802557953637No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2030.3957809362266284No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2000.38993195687352555No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2000.38993195687352555No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC2000.38993195687352555No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1970.3840829775204227No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1950.38018365795168746No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1940.3782339981673198No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1900.3704353590298493No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1870.3645863796767464No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1870.3645863796767464No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1850.36068706010801116No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1840.35873740032364354No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1830.3567877405392759No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1820.35483808075490825No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC1800.350938761186173No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1730.3372911426955996No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.33144216334249677No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1690.3294925035581291No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1670.32559318398939385No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1610.3138952252831881No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1600.31194556549882047No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC1570.30609658614571755No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1520.2963482872238794No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1500.29244896765514417No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1500.29244896765514417No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1480.2885496480864089No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1480.2885496480864089No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1460.2846503285176737No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1430.2788013491645708No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1420.27685168938020316No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1390.27100271002710025No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1370.267103390458365No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1320.25735509153652686No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC1310.25540543175215924No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1300.2534557719677916No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.2398081534772182No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1220.23785849369285061No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1200.23395917412411532No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1160.22616053498664482No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1130.22031155563354193No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC1130.22031155563354193No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1110.2164122360648067No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1110.2164122360648067No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC1100.21446257628043908No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1100.21446257628043908No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC1070.2086135969273362No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC1070.2086135969273362No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC1040.2027646175742333No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1030.2008149577898657No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1020.19886529800549804No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1020.19886529800549804No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1020.19886529800549804No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC1010.1969156382211304No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1000.19496597843676278No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT980.1910666588680275No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT970.1891169990836599No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT970.1891169990836599No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT970.1891169990836599No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTCGATACCCGCACC930.1813183599461894No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG930.1813183599461894No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA920.17936870016182177No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT910.17741904037745412No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA910.17741904037745412No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT910.17741904037745412No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC880.17157006102435124No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT860.167670741455616No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT850.16572108167124838No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT840.16377142188688074No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT830.16182176210251312No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTCGATACCCGCACC830.16182176210251312No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA830.16182176210251312No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA820.15987210231814547No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.15987210231814547No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT820.15987210231814547No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.15597278274941023No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT790.1540231229650426No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCCGGCGTTA780.15207346318067497No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT780.15207346318067497No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA780.15207346318067497No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA760.1481741436119397No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT750.14622448382757208No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC750.14622448382757208No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.14427482404320446No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC740.14427482404320446No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA710.13842584469010158No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC700.13647618490573396No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.1345265251213663No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.13062720555263105No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT670.13062720555263105No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT660.12867754576826343No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA650.1267278859838958No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG630.12282856641516056No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT620.12087890663079293No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT620.12087890663079293No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA600.11697958706205766No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT600.11697958706205766No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT590.11502992727769004No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA590.11502992727769004No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT590.11502992727769004No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.11502992727769004No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC580.11308026749332241No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.11113060770895479No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.11113060770895479No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.10918094792458716No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT540.10528162835585189No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT540.10528162835585189No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT530.10333196857148427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)