FastQCFastQC Report
Sun 21 May 2023
EGAF00007847524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35713
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA34079.539943437963768No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA20195.653403522526811No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA19195.373393442163918No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10973.071710581580937No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT9152.562092235320472No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9112.5508918321059557No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8642.419287094335396No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6831.9124688488785595No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6511.8228656231624336No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6091.7052613894100188No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5081.4224512082434968No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4731.3244476801164842No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4621.293646571276566No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4601.288046369669308No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4451.2460448576148742No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4081.1424411278806037No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3941.1032397166297987No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3891.089239212611654No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3140.8792316523394843No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2950.8260297370705345No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2940.8232296362669056No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2860.8008288298378743No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2830.7924285274269873No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2750.7700277209979559No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2720.7616274185870692No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2610.7308263097471509No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2580.7224260073362642No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2480.6944249992999748No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2410.6748242936745722No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2220.6216223784056226No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2200.6160221767983648No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2170.607621874387478No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2160.604821773583849No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2160.604821773583849No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2030.5684204631366729No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT2010.562820261529415No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1970.5516198583148993No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1940.5432195559040126No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1780.4984179430459496No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1620.4536163301878868No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1600.4480161285806289No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1560.43681572536611324No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.43401562456248427No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1540.4312155237588553No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1470.4116148181334528No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1460.4088147173298239No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1410.3948142133116792No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1340.3752135076862767No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1300.364013104471761No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1300.364013104471761No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1290.3612130036681321No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1280.3584129028645031No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1270.3556128020608742No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1210.3388121972391006No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1200.33601209643547164No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1190.33321199563184273No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1180.3304118948282138No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1170.3276117940245849No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1120.3136112900064402No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1100.3080110883991824No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.30521098759555343No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.2940105843810377No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT980.2744098787556352No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA910.2548091731302327No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG880.2464088707193459No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT880.2464088707193459No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC870.24360876991571695No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA850.23800856830845912No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA850.23800856830845912No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA840.23520846750483018No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA830.2324083667012012No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC820.2296082658975723No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC820.2296082658975723No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT810.2268081650939434No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC810.2268081650939434No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA800.22400806429031445No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT790.22120796348668553No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC770.21560776187942765No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG760.2128076610757987No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.2128076610757987No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT750.2100075602721698No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT750.2100075602721698No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA750.2100075602721698No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG740.20720745946854086No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.20720745946854086No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA710.19880715705765406No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC680.1904068546467673No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG670.18760675384313835No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.1820065522358805No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT640.17920645143225156No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA630.17640635062862262No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC620.1736062498249937No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA620.1736062498249937No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC610.17080614902136476No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT600.16800604821773582No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC590.1652059474141069No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA590.1652059474141069No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA590.1652059474141069No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT580.162405846610478No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC560.1568056450032201No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.1568056450032201No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC540.15120544339596226No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT540.15120544339596226No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.14280514098507546No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA500.14000504018144652No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT500.14000504018144652No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA490.1372049393778176No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC480.13440483857418867No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA470.13160473777055973No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC450.1260045361633019No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT440.12320443535967295No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC440.12320443535967295No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT420.11760423375241509No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT420.11760423375241509No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT410.11480413294878615No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT410.11480413294878615No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.11480413294878615No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.11480413294878615No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG410.11480413294878615No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.11480413294878615No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC400.11200403214515722No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA400.11200403214515722No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT380.10640383053789935No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA380.10640383053789935No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT380.10640383053789935No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT380.10640383053789935No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC370.10360372973427043No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC370.10360372973427043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)