FastQCFastQC Report
Sun 21 May 2023
EGAF00007847525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35713
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA31808.904320555539998No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA18815.266989611626018No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA18415.1549855794808614No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT9392.6292946546075657No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9362.620894352196679No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8942.503290118444264No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8622.4136868927281383No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT7912.214879735670484No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7202.0160725786128304No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT5731.604457760479377No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5411.5148545347632514No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4861.3608489905636603No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4831.3524486881527735No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4521.2656455632402765No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4481.254445160025761No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4201.1760423375241509No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3841.0752387085935093No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3661.0248368941281887No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3550.9940357852882704No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3240.9072326603757735No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3230.9044325595721446No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3060.8568308459104527No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2900.8120292330523899No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2660.7448268137652955No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2660.7448268137652955No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2620.7336264105507798No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2610.7308263097471509No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2600.728026208943522No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2520.7056254025144905No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2410.6748242936745722No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2380.6664239912636855No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2160.604821773583849No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2140.5992215719765911No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1930.5404194551003837No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1890.5292190518858678No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1760.4928177414386918No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1750.49001764063506287No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1710.47881723742054716No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1710.47881723742054716No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA1690.47321703581328933No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1580.44241592697337107No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1530.42841542295522633No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1490.4172150197407107No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1480.4144149189370817No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.4144149189370817No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1470.4116148181334528No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1460.4088147173298239No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1450.40601461652619497No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1440.40321451572256606No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1410.3948142133116792No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1400.39201411250805024No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1380.3864139109007924No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.3808137092935346No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1360.3808137092935346No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1250.3500126004536163No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1200.33601209643547164No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1200.33601209643547164No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1190.33321199563184273No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT1090.30521098759555343No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1050.2940105843810377No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1050.2940105843810377No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1050.2940105843810377No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1050.2940105843810377No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1040.2912104835774088No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1030.2884103827737799No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1030.2884103827737799No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA960.26880967714837734No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT930.26040937473749054No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC930.26040937473749054No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT910.2548091731302327No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT900.2520090723266038No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA870.24360876991571695No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT860.24080866911208804No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA860.24080866911208804No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.2268081650939434No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT790.22120796348668553No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT790.22120796348668553No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.22120796348668553No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG780.21840786268305662No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC740.20720745946854086No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC740.20720745946854086No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA730.20440735866491194No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA710.19880715705765406No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC710.19880715705765406No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA700.19600705625402512No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.1932069554503962No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT690.1932069554503962No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA680.1904068546467673No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG650.1820065522358805No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA630.17640635062862262No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG610.17080614902136476No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC600.16800604821773582No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA580.162405846610478No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT580.162405846610478No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA580.162405846610478No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC570.15960574580684903No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA550.1540055441995912No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT520.1456052417887044No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT520.1456052417887044No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.1456052417887044No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC520.1456052417887044No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC510.14280514098507546No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC510.14280514098507546No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC500.14000504018144652No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA500.14000504018144652No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA490.1372049393778176No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT490.1372049393778176No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG480.13440483857418867No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.13160473777055973No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.1288046369669308No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA460.1288046369669308No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA460.1288046369669308No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA450.1260045361633019No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA450.1260045361633019No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC450.1260045361633019No Hit
ATACCAGGTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTA440.12320443535967295No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC430.12040433455604402No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT430.12040433455604402No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT430.12040433455604402No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA420.11760423375241509No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC410.11480413294878615No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.11480413294878615No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT400.11200403214515722No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA390.10920393134152831No Hit
ATACCAGGTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA390.10920393134152831No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT390.10920393134152831No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC380.10640383053789935No Hit
ATACCAGGTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG370.10360372973427043No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA370.10360372973427043No Hit
ATACCAGGTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA360.10080362893064151No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC360.10080362893064151No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC360.10080362893064151No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC360.10080362893064151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)