FastQCFastQC Report
Sun 21 May 2023
EGAF00007847532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28261
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA14385.08828420791904No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA13764.86890060507413No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8282.9298326315417005No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA7192.54414210395952No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5441.924914192703726No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5311.8789144050104383No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5251.8576837337673826No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4221.493223877428258No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3921.387070521212979No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3881.3729167403842752No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3721.3163016170694597No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3521.2455327129259404No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3371.1924560348183009No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3351.185379144403949No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3331.1783022539895969No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3271.157071582746541No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3091.0933795690173738No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2710.958918651144687No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2680.9483033155231592No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2670.9447648703159832No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2550.9023035278298717No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2410.8527652949294081No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2330.8244577332720002No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT2310.8173808428576482No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2290.8103039524432963No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2280.8067655072361204No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2250.7961501716145925No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2200.7784579455787127No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2170.7678426099571849No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT2120.750150383921305No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2100.743073493506953No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2000.7076890414351934No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1970.6970737058136655No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1870.6616892537419058No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1630.5767665687696826No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1630.5767665687696826No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1620.5732281235625066No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1540.5449205619050989No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1520.537843671490747No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1460.5166130002476912No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1430.5059976646261632No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1410.49892077421181136No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1400.49538232900463536No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1380.4883054385902834No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1370.48476699338310747No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1360.4812285481759315No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1360.4812285481759315No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1340.4741516577615796No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1310.4635363221400517No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1310.4635363221400517No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1300.4599978769328757No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1220.431690315275468No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1190.4210749796539401No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1170.4139980892395881No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1150.40692119882523614No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1060.3750751919606525No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.3715367467534765No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.35738296592477264No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1000.3538445207175967No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG950.33615229468171687No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA940.33261384947454087No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT920.325536959060189No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT920.325536959060189No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA910.321998513853013No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA860.30430628781713315No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT850.3007678426099572No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA840.29722939740278126No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG830.2936909521956052No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT830.2936909521956052No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA830.2936909521956052No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT820.29015250698842926No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT820.29015250698842926No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA790.27953717136690137No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT790.27953717136690137No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC780.2759987261597254No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA700.24769116450231768No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT700.24769116450231768No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA700.24769116450231768No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA680.24061427408796576No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC680.24061427408796576No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA670.2370758288807898No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT670.2370758288807898No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC660.23353738367361382No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA660.23353738367361382No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT650.22999893846643785No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT650.22999893846643785No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG640.2264604932592619No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA640.2264604932592619No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT630.22292204805208593No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC620.21938360284490996No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA600.21230671243055804No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC590.20876826722338201No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC580.20522982201620607No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA570.2016913768090301No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC570.2016913768090301No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG560.19815293160185413No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC550.19461448639467818No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA540.1910760411875022No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT530.18753759598032624No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT530.18753759598032624No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA520.18399915077315027No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC520.18399915077315027No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT510.1804607055659743No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC510.1804607055659743No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC490.17338381515162238No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA490.17338381515162238No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT480.1698453699444464No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC480.1698453699444464No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT470.16630692473727043No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT470.16630692473727043No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA460.1627684795300945No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA460.1627684795300945No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.1627684795300945No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.15569158911574255No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT440.15569158911574255No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC430.15215314390856657No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT430.15215314390856657No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA420.14861469870139063No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA420.14861469870139063No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTA420.14861469870139063No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA420.14861469870139063No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC410.14507625349421463No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC400.14153780828703866No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTA390.1379993630798627No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC380.13446091787268674No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC380.13446091787268674No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA370.13092247266551077No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA370.13092247266551077No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA360.1273840274583348No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT360.1273840274583348No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC360.1273840274583348No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT350.12384558225115884No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA350.12384558225115884No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA340.12030713704398288No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA340.12030713704398288No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.12030713704398288No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA330.11676869183680691No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT330.11676869183680691No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT330.11676869183680691No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA330.11676869183680691No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT330.11676869183680691No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT330.11676869183680691No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT320.11323024662963095No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA320.11323024662963095No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC320.11323024662963095No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT320.11323024662963095No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT310.10969180142245498No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG310.10969180142245498No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT310.10969180142245498No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT300.10615335621527902No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA300.10615335621527902No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA300.10615335621527902No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA290.10261491100810304No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA290.10261491100810304No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA290.10261491100810304No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA290.10261491100810304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)