FastQCFastQC Report
Sun 21 May 2023
EGAF00007847533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28261
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA12054.26382647464704No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA11954.228442022575281No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7202.5476805491666963No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6572.3247585011146104No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6142.1726053572060438No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6012.126605569512756No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5742.031067548919005No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4421.5639927815717773No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4071.4401471993206185No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4001.4153780828703868No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3821.3516860691412194No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3711.3127631718622836No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3701.3092247266551078No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA3631.284455610204876No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3331.1783022539895969No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3001.06153356215279No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2871.0155337744595025No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.9907646580092707No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.9836877675949188No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2580.9129188634513995No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2510.8881497470011678No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2450.866919075758112No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.8421499593078802No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA2260.7996886168217685No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2230.7890732812002406No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2110.746611938714129No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2020.7147659318495453No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT1990.7041505962280173No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1940.6864583701921376No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1930.6829199249849616No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1920.6793814797777856No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1900.6723045893634337No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1870.6616892537419058No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1820.643997027706026No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT1800.6369201372916741No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT1790.6333816920844981No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1780.6298432468773221No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1730.6121510208414424No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT1700.6015356852199144No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.5909203495983865No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1670.5909203495983865No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1550.5484590071122748No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1490.527228335869219No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1490.527228335869219No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1470.5201514454548671No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1460.5166130002476912No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1430.5059976646261632No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1390.4918438837974594No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1350.4776901029687555No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1240.4387672056898199No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.431690315275468No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1220.431690315275468No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1200.4246134248611161No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1170.4139980892395881No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1150.40692119882523614No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1140.4033827536180602No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.38922897278935636No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1100.38922897278935636No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1040.36799830154630053No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG980.34676763030324476No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC940.33261384947454087No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA920.325536959060189No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT920.325536959060189No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA920.325536959060189No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT910.321998513853013No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT900.31846006864583704No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA870.30784473302430915No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA860.30430628781713315No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA860.30430628781713315No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT850.3007678426099572No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC840.29722939740278126No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT840.29722939740278126No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA830.2936909521956052No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT810.2866140617812533No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA760.2689218357453735No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA740.26184494533102154No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT740.26184494533102154No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA730.2583065001238456No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC710.25122960970949365No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT710.25122960970949365No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA710.25122960970949365No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA690.2441527192951417No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT690.2441527192951417No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC690.2441527192951417No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA640.2264604932592619No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT630.22292204805208593No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT620.21938360284490996No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA620.21938360284490996No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.21938360284490996No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT620.21938360284490996No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT610.215845157637734No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT590.20876826722338201No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA580.20522982201620607No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.2016913768090301No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC570.2016913768090301No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA570.2016913768090301No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG560.19815293160185413No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA560.19815293160185413No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC530.18753759598032624No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC520.18399915077315027No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC510.1804607055659743No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG500.17692226035879835No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA500.17692226035879835No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC500.17692226035879835No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT500.17692226035879835No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC480.1698453699444464No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC470.16630692473727043No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT470.16630692473727043No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT470.16630692473727043No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC460.1627684795300945No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA460.1627684795300945No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTA460.1627684795300945No Hit
ATGTTGGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA460.1627684795300945No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.15923003432291852No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTA440.15569158911574255No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT430.15215314390856657No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.15215314390856657No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC420.14861469870139063No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA420.14861469870139063No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT420.14861469870139063No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA420.14861469870139063No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA410.14507625349421463No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT400.14153780828703866No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT390.1379993630798627No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA380.13446091787268674No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC380.13446091787268674No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC380.13446091787268674No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT370.13092247266551077No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA370.13092247266551077No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACT360.1273840274583348No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC360.1273840274583348No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA350.12384558225115884No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC350.12384558225115884No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT350.12384558225115884No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA350.12384558225115884No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT350.12384558225115884No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT340.12030713704398288No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTA340.12030713704398288No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA340.12030713704398288No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA340.12030713704398288No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT340.12030713704398288No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA330.11676869183680691No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA330.11676869183680691No Hit
ATGTTGGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA320.11323024662963095No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT320.11323024662963095No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT320.11323024662963095No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA320.11323024662963095No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT310.10969180142245498No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT310.10969180142245498No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT310.10969180142245498No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA310.10969180142245498No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA300.10615335621527902No Hit
ATGTTGGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA290.10261491100810304No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT290.10261491100810304No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT290.10261491100810304No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT290.10261491100810304No Hit
ATGTTGGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTA290.10261491100810304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)