FastQCFastQC Report
Sun 21 May 2023
EGAF00007847546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39255
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12053.0696726531652017No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA10992.7996433575340722No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8192.0863584256782577No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7341.8698254999363138No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6271.5972487581199846No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6071.546299834415998No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5401.3756209400076425No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5061.2890077697108648No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5061.2890077697108648No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4911.2507960769328748No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4811.2253216150808814No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4631.1794675837472934No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4491.1438033371545027No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4401.1208763214877084No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4391.1183288753025091No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4371.1132339829321107No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4351.108139090561712No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA4221.0750222901541204No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4141.0546427206725257No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4081.0393580435613299No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4011.0215259202649345No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3961.0087886893389377No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3920.9985989045981404No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3910.996051458412941No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3900.9935040122277417No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA3750.9552923194497517No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3520.8967010571901669No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3430.8737740415233728No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3430.8737740415233728No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3400.8661317029677749No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3360.8559419182269774No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3330.8482995796713794No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3230.8228251178193862No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3160.8049929945229908No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3160.8049929945229908No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2970.7565915170042032No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2960.7540440708190039No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2920.7438542860782066No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2850.7260221627818112No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2800.7132849318558145No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA2740.6980002547446186No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2730.6954528085594192No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA2660.6776206852630238No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2640.6725257928926252No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2630.6699783467074257No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2530.6445038848554324No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2490.6343141001146351No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2440.6215768691886384No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2370.603744745892243No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2160.550248376003057No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2030.5171315755954655No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1960.4992994522990702No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1890.48146732900267486No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1770.4508979747802827No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1690.43051840529868807No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.4254235129282894No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.4101388358170934No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1580.4024964972614954No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.399949051076296No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1540.392306712520698No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1520.38721182015029937No Hit
TCAGGTTCCCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1520.38721182015029937No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1470.3744745892243026No Hit
TCAGGTTCCCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1440.3668322506687046No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1430.3642848044835053No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1400.35664246592790727No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1400.35664246592790727No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1370.3490001273723093No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1370.3490001273723093No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1350.3439052350019106No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1330.3388103426315119No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1300.3311680040759139No Hit
TCAGGTTCCCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.3184307731499172No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1240.3158833269647178No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1240.3158833269647178No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1220.3107884345943192No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.30569354222392053No Hit
TCAGGTTCCCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.30569354222392053No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1110.2827665265571265No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT1080.2751241880015285No Hit
TCAGGTTCCCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1050.26748184944593045No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1050.26748184944593045No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1040.2649344032607311No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1030.2623869570755318No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1010.2572920647051331No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1010.2572920647051331No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1000.2547446185199338No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT1000.2547446185199338No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1000.2547446185199338No Hit
TCAGGTTCCCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.2547446185199338No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT990.25219717233473443No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA990.25219717233473443No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA990.25219717233473443No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT980.2496497261495351No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA940.23945994140873775No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA930.2369124952235384No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA920.23436504903833907No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT910.2318176028531397No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC900.22927015666794037No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA900.22927015666794037No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT880.22417526429754173No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC880.22417526429754173No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT850.21653292574194372No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA830.21143803337154501No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC820.20889058718634568No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT800.20379569481594703No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT800.20379569481594703No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT800.20379569481594703No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC790.2012482486307477No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.19870080244554833No Hit
TCAGGTTCCCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.196153356260349No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA770.196153356260349No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT760.19360591007514968No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT750.19105846388995032No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT740.18851101770475098No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA730.18596357151955165No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT700.17832123296395364No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA690.1757737867787543No Hit
TCAGGTTCCCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT680.17322634059355496No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA680.17322634059355496No Hit
TCAGGTTCCCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT680.17322634059355496No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA670.17067889440835562No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT660.1681314482231563No Hit
TCAGGTTCCCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT650.16558400203795695No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.16558400203795695No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA650.16558400203795695No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA640.16303655585275761No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA630.16048910966755828No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCTAGCT630.16048910966755828No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA630.16048910966755828No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.1579416634823589No Hit
TCAGGTTCCCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT620.1579416634823589No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA610.1553942172971596No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.1553942172971596No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.1553942172971596No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT600.15284677111196027No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.1502993249267609No Hit
TCAGGTTCCCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC590.1502993249267609No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC590.1502993249267609No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAACACGTAAGGTGCGAGCGTTG590.1502993249267609No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.14520443255636226No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC570.14520443255636226No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC540.13756209400076425No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC530.1350146478155649No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT510.12991975544516624No Hit
TCAGGTTCCCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC500.1273723092599669No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCTAGCT490.12482486307476755No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG490.12482486307476755No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT490.12482486307476755No Hit
TCAGGTTCCCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT480.1222774168895682No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT480.1222774168895682No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA470.11972997070436887No Hit
TCAGGTTCCCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT470.11972997070436887No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.11463507833397019No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA450.11463507833397019No Hit
TCAGGTTCCCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCACGCT440.11208763214877086No Hit
TCAGGTTCCCCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT440.11208763214877086No Hit
TCAGGTTCCCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT430.10954018596357153No Hit
TCAGGTTCCCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC430.10954018596357153No Hit
TCAGGTTCCCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCT430.10954018596357153No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA410.10444529359317284No Hit
TCAGGTTCCCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.10444529359317284No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA410.10444529359317284No Hit
TCAGGTTCCCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.10189784740797352No Hit
TCAGGTTCCCCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT400.10189784740797352No Hit
TCAGGTTCCCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC400.10189784740797352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)