FastQCFastQC Report
Sun 21 May 2023
EGAF00007847577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49609
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA15613.1466064625370396No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA15233.070007458324094No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT12382.4955149267270054No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11112.239512991594267No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9171.848454917454494No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA9041.8222499949605917No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8911.7960450724666894No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8621.7375879376725998No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA8101.6327682476969905No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT7791.5702795863653771No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7401.4916648188836703No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA6951.400955471789393No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA6921.3949081819831077No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT6001.20945796125703No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5961.2013949081819832No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5661.1409220101191317No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5541.116732850893991No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5491.1066540345501825No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5341.0764175855187568No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5181.0441653732185692No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5161.0401338466810457No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA4790.9655506057368624No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA4780.9635348424681006No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4620.9312826301679131No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4520.9111249974802959No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4490.9050777076740109No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4440.8949988913302022No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4430.8929831280614404No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4200.846620572879921No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4190.8446048096111592No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3990.8042895442359249No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3850.7760688584732609No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3840.7740530952044992No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3840.7740530952044992No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3720.7498639359793586No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3680.7418008829043118No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3560.7176117236791711No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3540.7135801971416477No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3480.7014856175290773No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3470.6994698542603156No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3190.6430284827349876No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3140.6329496663911791No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3120.6289181398536556No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3080.6208550867786087No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2940.5926344010159447No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2920.5886028744784212No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2800.5644137152532807No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT2790.562397951984519No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2760.5563506621782338No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2740.5523191356407103No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2730.5503033723719486No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2580.5200669233405228No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2540.512003870265476No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2530.5099881069967143No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT2510.5059565804591909No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2340.47168860489024167No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2340.47168860489024167No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2320.4676570783527183No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2310.46564131508395656No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2280.4595940252776714No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2140.43137333951500734No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2140.43137333951500734No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2100.4233102864399605No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2040.4112157068273902No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2030.4091999435586285No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1990.4011368904835816No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1940.391058074139773No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1900.38299502106472616No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1890.3809792577959644No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1830.36888467818339415No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1730.34872704549577693No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1720.34671128222701525No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1700.3426797556894918No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1670.33663246588320667No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1660.334616702614445No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1640.3305851760769215No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1620.3265536495393981No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1600.32252212300187466No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1580.31849059646435124No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.31647483319558956No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1480.2983329637767341No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1480.2983329637767341No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1420.28623838416416375No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1410.28422262089540207No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1410.28422262089540207No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1400.28220685762664033No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1360.27414380455159343No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1350.27212804128283175No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1340.27011227801407No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1310.2640649882077849No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.2600334616702614No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1240.24995464532645284No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.24793888205769116No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT1200.24189159225140602No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1190.23987582898264428No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1170.23584430244512083No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1160.23382853917635915No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1130.22778124937007396No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1130.22778124937007396No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1130.22778124937007396No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT1120.22576548610131228No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1110.22374972283255054No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1100.22173395956378883No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1080.2177024330262654No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1060.21367090648874199No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1060.21367090648874199No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.21165514321998025No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.20157632687617166No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT990.19956056360740992No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT990.19956056360740992No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT950.19149751053236308No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA950.19149751053236308No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT940.18948174726360137No Hit
TCCTTGGCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT930.18746598399483966No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT930.18746598399483966No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT900.1814186941885545No Hit
TCCTTGGCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT890.17940293091979279No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA880.17738716765103107No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT860.17335564111350762No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT860.17335564111350762No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT840.1693241145759842No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA800.16126106150093733No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT790.15924529823217562No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT770.15521377169465217No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA760.15319800842589046No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA750.15118224515712875No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA750.15118224515712875No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA720.1451349553508436No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA700.14110342881332016No Hit
TCTCCGCACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA700.14110342881332016No Hit
TCCTTGGCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT690.13908766554455845No Hit
TCTCCGCACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG680.13707190227579671No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC660.1330403757382733No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.1330403757382733No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT630.12699308593198813No Hit
TCTCCGCACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG620.12497732266322642No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.12497732266322642No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC610.12296155939446472No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT600.12094579612570301No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC600.12094579612570301No Hit
TCCTTGGCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT600.12094579612570301No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA600.12094579612570301No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC590.11893003285694129No Hit
TCTCCGCACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA570.11489850631941785No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.11489850631941785No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT560.11288274305065614No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCCGTTTGCTCCCCTGGCT530.10683545324437099No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.10683545324437099No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.10481968997560927No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT520.10481968997560927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)