FastQCFastQC Report
Sun 21 May 2023
EGAF00007847584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54547
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA17113.1367444589069975No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11182.049608594423158No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10461.9176123343171942No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA9551.7507837277943792No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9541.7489504464040184No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8801.6132876235173337No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8111.4867912075824519No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT7741.4189597961391094No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7361.3492951033054064No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7131.3071296313271124No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6841.2539644710066549No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6421.1769666526115095No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6251.1458008689753791No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG6231.1421343061946578No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6121.1219682109006912No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6071.112801803948888No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5901.0816360203127577No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5871.076136176141676No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5731.0504702366766274No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5541.015637890259776No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5491.0064714833079729No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5210.955139604377876No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5090.9331402276935488No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5090.9331402276935488No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT5020.9203072579610245No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA4900.8983078812766973No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA4760.8726419418116487No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4700.861642253469485No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4690.8598089720791244No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4660.8543091279080426No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4320.7919775606357821No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4310.7901442792454213No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4270.782811153683979No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4240.7773113095128972No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA4200.7699781839514548No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4120.7553119328285699No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4070.7461455258767669No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA4070.7461455258767669No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4040.740645681705685No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3560.6526481749683759No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3470.6361486424551305No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3450.6324820796744093No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3440.6306487982840486No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3440.6306487982840486No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3420.6269822355033274No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3360.6159825471611637No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3310.6068161402093607No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3240.5939831704768365No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA3110.5701505124021485No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2980.5463178543274607No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA2950.5408180101563789No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2900.5316516032045758No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2810.5151520706913304No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2730.5004858195684456No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2640.4839862870552001No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2610.4784864428841183No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2600.47665316149375764No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA2570.4711533173226759No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2550.46748675454195465No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2550.46748675454195465No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2460.4509872220287092No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2450.4491539406383486No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2340.42898784534438195No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2330.4271545639540213No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2320.4253212825636607No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2290.4198214383925789No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2280.4179881570022183No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA2260.41432159422149706No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2250.4124883128311364No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2150.3941554989275304No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA2130.3904889361468092No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1930.353823308339597No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1930.353823308339597No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1900.34832346416851523No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1800.32999065026490915No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1780.32632408748418795No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1770.32449080609382736No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1720.3153243991420243No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1670.3061579921902213No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1670.3061579921902213No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA1670.3061579921902213No Hit
TACACATGACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1670.3061579921902213No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1640.3006581480191394No Hit
TACACATGACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1570.28782517828661525No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1570.28782517828661525No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1560.2859918968962546No Hit
TACACATGACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1540.2823253341155334No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1510.27682548994445155No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1490.27315892716373036No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1490.27315892716373036No Hit
TACACATGACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1480.27132564577336976No Hit
TACACATGACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1450.2658258016022879No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT1380.2529928318697637No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1380.2529928318697637No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT1360.24932626908904248No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1310.24015986213723944No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1310.24015986213723944No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1300.23832658074687882No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1300.23832658074687882No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1270.232826736575797No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1210.22182704823363336No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC1200.2199937668432728No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCT1200.2199937668432728No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1160.21266064128183035No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1150.21082735989146972No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT1140.20899407850110915No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT1130.20716079711074853No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA1110.20349423433002733No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCT1080.19799439015894552No Hit
TACACATGACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.18882798320714247No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.18332813903606066No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC980.17966157625533943No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT960.1759950134746182No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCT940.172328450693897No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT920.1686618879131758No Hit
TACACATGACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT910.16682860652281517No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT880.16132876235173335No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA880.16132876235173335No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC850.15582891818065153No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.1503290740095697No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.1503290740095697No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT820.1503290740095697No Hit
TACACATGACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT820.1503290740095697No Hit
TACACATGACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT820.1503290740095697No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA800.14666251122884852No Hit
TACACATGACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC800.14666251122884852No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT790.14482922983848792No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG790.14482922983848792No Hit
TACACATGACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT780.1429959484481273No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT770.1411626670577667No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA740.13566282288668488No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.13382954149632428No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA730.13382954149632428No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG730.13382954149632428No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT710.13016297871560306No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT710.13016297871560306No Hit
TACACATGACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.13016297871560306No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA700.12832969732524246No Hit
TACACATGACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT690.12649641593488184No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA690.12649641593488184No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCT680.12466313454452124No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.12282985315416063No Hit
TCAGTCGACGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA660.12099657176380002No Hit
TCAGTCGACGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA660.12099657176380002No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.11916329037343941No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC650.11916329037343941No Hit
TACACATGACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC640.11733000898307881No Hit
TACACATGACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.11733000898307881No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA640.11733000898307881No Hit
TACACATGACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC640.11733000898307881No Hit
TACACATGACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC640.11733000898307881No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.11549672759271822No Hit
TACACATGACCGGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCT630.11549672759271822No Hit
TCAGTCGACGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA620.11366344620235759No Hit
TACACATGACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC610.111830164811997No Hit
TACACATGACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC610.111830164811997No Hit
TCAGTCGACGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA610.111830164811997No Hit
TACACATGACCGGACTACCCGGGTATCTAATCCTGTTCGATACCCGCACC600.1099968834216364No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC590.10816360203127577No Hit
TACACATGACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACT570.10449703925055458No Hit
TACACATGACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.10449703925055458No Hit
TACACATGACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.10083047646983335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)