FastQCFastQC Report
Sun 21 May 2023
EGAF00007847594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55035
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA36846.693922049604797No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA25074.555283001726174No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA16693.032615608249296No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA12622.293086217861361No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT12452.2621967838648134No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11932.1677114563459616No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA11802.1440901244662487No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG10071.8297447079131464No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG9451.7170891251022076No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8631.5680930317070956No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT8431.531752521122922No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT7141.2973562278550015No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT7131.2955392023257926No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6851.2446624875079495No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG6711.2192241300990279No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA6361.1556282365767239No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6121.1120196238757156No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5611.0193513218860724No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5450.9902789134187335No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5430.9866448623603162No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5160.9375851730716817No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5070.9212319433088034No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4940.8976106114290906No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4920.8939765603706733No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4890.8885254837830472No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4810.8739892795493776No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4770.866721177432543No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4760.8649041519033343No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4650.8449168710820387No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4560.8285636413191605No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT4430.8049423094394477No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4040.7340783138003089No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3920.7122740074498046No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3880.7050059053329699No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3800.6904697010993005No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3740.6795675479240484No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3740.6795675479240484No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3610.6559462160443355No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3390.6159716544017443No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3330.6050695012264923No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3290.5978013991096575No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3260.5923503225220315No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3240.588716271463614No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3200.5814481693467793No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT2990.5432906332333969No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2950.5360225311165622No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2890.5251203779413101No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2890.5251203779413101No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2880.5233033524121014No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2830.5142182247660579No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2790.5069501226492232No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2760.5014990460615972No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2510.45607340783138No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2410.4379031525392932No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2390.43426910148087583No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2350.427000999364041No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2240.40701371854274554No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2220.40337966748432813No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2140.38884346325065866No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2130.38702643772144996No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2100.38157536113382395No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT2090.37975833560461525No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2070.3761242845461979No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2050.37249023348778054No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2030.36885618242936313No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1970.357954029254111No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1960.3561370037249023No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1930.3506859271372763No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1920.3488689016080676No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1910.3470518760788589No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1860.3379667484328155No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1760.3197964931407286No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1740.31616244208231126No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1700.30889433996547655No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1620.294358135731807No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.2870900336149723No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1580.2870900336149723No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1550.28163895702734626No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1510.2743708549105115No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1500.2725538293813028No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1440.2616516762060507No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1380.2507495230307986No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1370.2489324975015899No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1360.2471154719723812No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.23803034432633777No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1260.22894521668029433No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1230.22349414009266833No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.22167711456345962No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1200.21804306350504227No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1190.21622603797583356No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1170.21259198691741618No Hit
TCCTTGGCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1160.21077496138820748No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1140.20714091032979012No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1130.20532388480058147No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1100.1998728082129554No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1090.19805578268374668No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1080.19623875715453803No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1050.19078768056691198No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1050.19078768056691198No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1020.18533660397928592No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT950.17261742527482513No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC940.17080039974561645No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT930.16898337421640774No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT910.16534932315799036No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA910.16534932315799036No Hit
TCCTTGGCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT900.1635322976287817No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC900.1635322976287817No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA900.1635322976287817No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA880.1598982465703643No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT860.15626419551194695No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT850.15444716998273827No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA840.15263014445352957No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT840.15263014445352957No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT830.15081311892432087No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT830.15081311892432087No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA820.1489960933951122No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.1489960933951122No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA810.1471790678659035No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.14536204233669484No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC790.14354501680748616No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT790.14354501680748616No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT770.13991096574906878No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.1362769146906514No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC740.13445988916144272No Hit
TGTACCAGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA740.13445988916144272No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT700.12719178704460798No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC700.12719178704460798No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG690.1253747615153993No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT680.1235577359861906No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT670.12174071045698191No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT660.11992368492777324No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA650.11810665939856456No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT650.11810665939856456No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA640.11628963386935587No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC630.11447260834014716No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.11265558281093849No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.11265558281093849No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCT610.11083855728172981No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT600.10902153175252113No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT590.10720450622331244No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC590.10720450622331244No Hit
TGTACCAGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA580.10538748069410374No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA580.10538748069410374No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT580.10538748069410374No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCT570.10357045516489506No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.10357045516489506No Hit
TGTACCAGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA560.10175342963568638No Hit
TGTACCAGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA560.10175342963568638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)