FastQCFastQC Report
Sun 21 May 2023
EGAF00007847602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50104
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA28745.73606897652882No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA25335.055484592048539No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG17633.518681143222098No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG16733.3390547660865395No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA11862.367076480919687No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA10442.0836659747724733No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG9641.9239980839853104No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG9391.8741018681143222No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG8741.7443717068497524No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8391.6745170046303688No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8141.6246207887593807No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8031.6026664537761457No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7421.480919687050934No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7311.4589653520676993No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA7291.45497365479802No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6861.3691521634999202No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5471.091729203257225No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4990.9959284687849272No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4870.9719782851668529No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4650.9280696152003833No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4590.916094523391346No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT4390.8761775506945553No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4320.8622066102506786No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4260.8502315184416415No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4190.8362605779977648No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4190.8362605779977648No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4100.8182979402842088No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4010.800335302570653No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT3900.7783809675874181No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3610.7205013571770718No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3420.6825802331151205No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3390.676592687210602No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3320.6626217467667253No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3190.6366757145138113No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3060.6107296822608973No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3030.6047421363563787No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3010.6007504390866997No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2900.5787961041034648No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2880.5748044068337856No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2880.5748044068337856No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2820.5628293150247485No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2810.5608334663899089No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2780.5548459204853904No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2780.5548459204853904No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2710.5408749800415136No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.5308957368673161No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2640.5269040395976369No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2550.5089414018840811No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT2430.4849912182660067No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2420.4829953696311672No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2330.46503273191761135No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA2300.4590451860130928No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2290.4570493373782532No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2260.45106179147373465No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA2210.44108254829953697No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2190.4370908510298579No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA2150.42910745649049975No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2140.4271116078556602No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2060.411144818776944No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA2040.40715312150726485No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1980.3951780296982277No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1980.3951780296982277No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1960.3911863324285486No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1910.38120708925435093No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1910.38120708925435093No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1880.3752195433498323No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1790.35725690563627654No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1760.35126935973175794No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1740.34727766246207886No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1700.3392942679227207No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1680.33530257065304164No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1610.32133163020916494No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1600.3193357815743254No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1590.31733993293948587No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1520.3033689924956091No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1520.3033689924956091No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1510.30137314386076963No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1510.30137314386076963No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1470.29338974932141143No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1450.2893980520517324No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1440.2874022034168928No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1340.2674437170684975No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1320.26345201979881844No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1300.2594603225291394No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1230.24548938208526264No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1210.2414976848155836No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.23950183618074405No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1200.23950183618074405No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1190.23750598754590452No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.23351429027622544No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1140.22752674437170683No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1110.22153919846718823No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1100.21954334983234872No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1050.20956410665815103No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT1010.20158071211879292No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1000.1995848634839534No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT990.19758901484911384No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA960.19160146894459523No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA920.1836180744052371No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT910.18162222577039758No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT900.17962637713555804No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC890.1776305285007185No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA880.17563467986587897No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA870.17363883123103943No Hit
TCCTTGGCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT860.17164298259619992No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.16365958805684178No Hit
TCCTTGGCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT820.16365958805684178No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA800.1596678907871627No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA760.15168449624780456No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA760.15168449624780456No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.14569695034328597No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCT710.1417052530736069No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA700.13970940443876737No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT700.13970940443876737No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG700.13970940443876737No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA690.13771355580392783No Hit
TCCTTGGCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT680.1357177071690883No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC670.13372185853424876No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA670.13372185853424876No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA670.13372185853424876No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG660.13172600989940922No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT650.1297301612645697No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA650.1297301612645697No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA640.12773431262973017No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT640.12773431262973017No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG640.12773431262973017No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA630.1257384639948906No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA610.12174676672521156No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA610.12174676672521156No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT610.12174676672521156No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.12174676672521156No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA600.11975091809037203No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCT600.11975091809037203No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA580.11575922082069295No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT580.11575922082069295No Hit
TCCTTGGCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.11575922082069295No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.11575922082069295No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA580.11575922082069295No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT560.1117675235510139No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.1117675235510139No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA560.1117675235510139No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCT560.1117675235510139No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG540.10777582628133482No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT540.10777582628133482No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA520.10378412901165576No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC520.10378412901165576No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC510.10178828037681621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)