FastQCFastQC Report
Sun 21 May 2023
EGAF00007847603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50104
Sequences flagged as poor quality0
Sequence length250
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA24294.847916334025228No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA22674.524588855181223No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG15213.035685773590931No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG13052.6045824684655914No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT10252.045744850710522No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT9971.9898610889350152No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA8951.7862845281813828No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8641.7244132205013571No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8501.6964713396136037No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT8251.6465751237426152No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7701.536803448826441No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG7541.5048698706690085No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7041.4050774389270317No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6931.383123103943797No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6611.3192559476289318No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6541.305285007185055No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT6021.2015008781733993No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT5971.1915216349992017No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5311.0597956250997924No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5211.0398371387513972No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT4910.9799616797062112No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT4710.9400447070094204No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT4650.9280696152003833No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4630.924077917930704No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4350.8681941561551972No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4320.8622066102506786No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4240.8462398211719624No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT4210.8402522752674437No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4150.8282771834584065No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT3930.7843685134919367No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3550.7085262653680345No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3530.7045345680983554No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3470.6925594762893182No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT3450.6885677790196392No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT3370.6726009899409229No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3350.6686092926712438No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT3300.6586300494970461No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3250.6486508063228485No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT3250.6486508063228485No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3040.6067379849912182No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT3010.6007504390866997No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2990.5967587418170206No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2920.5827878013731439No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2900.5787961041034648No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2870.5728085581989462No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2690.5368832827718346No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2540.5069455532492416No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2420.4829953696311672No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2370.4730161264569695No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2350.46902442918729037No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2320.4630368832827718No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2320.4630368832827718No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2270.45305764010857413No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT2260.45106179147373465No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2250.4490659428388951No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT2250.4490659428388951No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2250.4490659428388951No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2120.4231199105859812No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA2080.41513651604662305No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2060.411144818776944No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT2030.40515727287242537No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA2000.3991697269679068No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1980.3951780296982277No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1960.3911863324285486No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1960.3911863324285486No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1940.38719463515886954No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT1870.37322369471499284No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1840.3672361488104742No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1840.3672361488104742No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1810.3612486029059556No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT1790.35725690563627654No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1670.33330672201820216No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1650.32931502474852303No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1650.32931502474852303No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1570.3133482356698068No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1570.3133482356698068No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1560.31135238703496726No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1550.3093565384001277No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1520.3033689924956091No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1520.3033689924956091No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1520.3033689924956091No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.29338974932141143No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1460.29139390068657195No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1440.2874022034168928No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1420.2834105061472138No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1400.27941880887753473No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1400.27941880887753473No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1370.2734312629730161No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1310.2614561711639789No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.2514769279897812No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.24548938208526264No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.24349353345042313No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1210.2414976848155836No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCT1190.23750598754590452No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTCGCTCCCCATGCT1130.22553089573686733No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1040.20756825802331152No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCT1000.1995848634839534No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1000.1995848634839534No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT980.1955931662142743No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC960.19160146894459523No Hit
TCCTTGGCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT940.18760977167491616No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA920.1836180744052371No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT860.17164298259619992No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.17164298259619992No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA850.16964713396136036No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT840.16765128532652082No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT830.1656554366916813No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA800.1596678907871627No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.1596678907871627No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT790.15767204215232317No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT790.15767204215232317No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT750.14968864761296502No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA750.14968864761296502No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT750.14968864761296502No Hit
TCCTTGGCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT740.1476927989781255No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG740.1476927989781255No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC730.14569695034328597No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.14569695034328597No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA690.13771355580392783No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCT680.1357177071690883No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC680.1357177071690883No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCT680.1357177071690883No Hit
TCCTTGGCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT670.13372185853424876No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA670.13372185853424876No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT660.13172600989940922No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC660.13172600989940922No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA650.1297301612645697No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.1297301612645697No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT650.1297301612645697No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA640.12773431262973017No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA640.12773431262973017No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA640.12773431262973017No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA630.1257384639948906No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA620.1237426153600511No Hit
TCCTTGGCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT600.11975091809037203No Hit
TGCTTGGCTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA600.11975091809037203No Hit
TGCTTGGCTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA590.1177550694555325No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.1177550694555325No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA560.1117675235510139No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA560.1117675235510139No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.10977167491617436No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT540.10777582628133482No Hit
TGCTTGGCTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA540.10777582628133482No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC540.10777582628133482No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCT540.10777582628133482No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC520.10378412901165576No Hit
TGCTTGGCTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA520.10378412901165576No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC520.10378412901165576No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.10178828037681621No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC510.10178828037681621No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT510.10178828037681621No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC510.10178828037681621No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC510.10178828037681621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)