FastQCFastQC Report
Sun 21 May 2023
EGAF00007847654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55775
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA25924.647243388614971No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA25734.61317794710892No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA18443.306140744060959No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA17193.082025997310623No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT11312.0277902285970417No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT11031.9775885253249665No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT8211.471985656656208No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7721.3841326759300763No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA7461.3375168086060063No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7261.3016584491259526No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT6851.2281488121918422No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6631.1887046167637831No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6271.1241595696996862No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6151.1026445540116538No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT5991.073957866427611No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT5771.034513670999552No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5641.0112057373375167No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5470.980726131779471No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG5370.9627969520394442No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG5180.9287315105333932No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5160.9251456745853877No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5100.9143881667413716No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT5030.9018377409233528No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4960.8892873151053339No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4960.8892873151053339No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4940.8857014791573286No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4790.8588077095472882No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4730.8480502017032722No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4720.8462572837292693No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4650.8337068579112505No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4620.8283281039892424No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4620.8283281039892424No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4420.7924697445091886No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT4340.7781264007171672No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4330.7763334827431645No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4170.7476467951591215No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3900.6992380098610489No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3800.681308830121022No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3730.6687584043030032No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT3720.6669654863290004No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3620.6490363065889735No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT3580.6418646346929628No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3520.6311071268489467No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3490.6257283729269386No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3390.6077991931869117No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3280.5880770954728821No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT3260.5844912595248768No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3150.5647691618108471No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3110.5575974899148364No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3110.5575974899148364No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2960.5307037203047961No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2840.5091887046167638No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2810.5038099506947556No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2760.49484536082474223No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2620.46974450918870464No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2540.4554011653966831No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2390.4285073957866428No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2360.42312864186463467No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2320.41595696996862397No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2270.4069923800986105No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.4034065441506051No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT2190.3926490363065889No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2150.3854773644105782No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT2080.3729269385925594No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1990.3567906768265352No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1930.34603316898251907No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1920.34424025100851635No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1910.34244733303451363No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1910.34244733303451363No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT1860.3334827431645002No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1710.30658897355445985No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1650.2958314657104437No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1600.2868668758404303No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1530.2743164500224115No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1500.2689376961004034No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1480.26535186015239803No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1410.2528014343343792No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1390.2492155983863738No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT1380.24742268041237112No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1340.24025100851636036No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1340.24025100851636036No Hit
TCCTTGGCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1330.23845809054235767No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1290.23128641864634691No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1290.23128641864634691No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1270.22770058269834156No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1240.22232182877633347No Hit
TCCTTGGCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1190.21335723890632002No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1180.21156432093231733No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.20797848498431198No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1120.2008068130883012No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1100.19722097714029585No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1100.19722097714029585No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.19184222321828776No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1060.19004930524428507No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1030.184670551322277No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC1020.1828776333482743No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1010.18108471537427162No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1010.18108471537427162No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1010.18108471537427162No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT990.17749887942626624No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT980.17570596145226355No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT980.17570596145226355No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT970.17391304347826086No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT960.17212012550425818No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.1685342895562528No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT930.1667413715822501No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT930.1667413715822501No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT920.16494845360824742No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT910.16315553563424473No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT910.16315553563424473No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT900.16136261766024204No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT900.16136261766024204No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT870.15598386373823397No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA860.15419094576423129No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT840.1506051098162259No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA840.1506051098162259No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA830.14881219184222322No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC830.14881219184222322No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.14701927386822053No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA800.14343343792021515No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC790.14164051994621246No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT790.14164051994621246No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA780.13984760197220977No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA780.13984760197220977No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT780.13984760197220977No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA760.1362617660242044No Hit
AACCGACCAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA760.1362617660242044No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT740.13267593007619902No Hit
AACCGACCAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA740.13267593007619902No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCATGCT710.12729717615419095No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA710.12729717615419095No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGATACCCACGCT700.12550425818018826No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA690.12371134020618556No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.12371134020618556No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.12371134020618556No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC680.12191842223218288No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGATACCCACGCT680.12191842223218288No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA670.12012550425818018No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT670.12012550425818018No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.12012550425818018No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT660.1183325862841775No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC650.11653966831017482No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC630.11295383236216944No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG620.11116091438816673No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC620.11116091438816673No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC610.10936799641416406No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT610.10936799641416406No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT600.10757507844016137No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT590.10578216046615867No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT590.10578216046615867No Hit
AACCGACCAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA590.10578216046615867No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT590.10578216046615867No Hit
AACCGACCAGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA580.10398924249215599No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT580.10398924249215599No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT580.10398924249215599No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.10398924249215599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)