FastQCFastQC Report
Sun 21 May 2023
EGAF00007847664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35675
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA9532.6713384723195515No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8982.5171688857743515No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7282.040644709180098No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6351.7799579537491241No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6131.718290119131044No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5871.6454099509460407No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT5581.5641205325858443No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5481.5360896986685353No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5321.491240364400841No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5181.4519971969166081No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5151.4435879467414157No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4731.3258584442887176No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4641.3006306937631396No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4361.222144358794674No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4131.1576734407848632No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4131.1576734407848632No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4091.1464611072179398No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3861.081990189208129No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3731.0455501051156273No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA3611.0119131044148564No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3581.0035038542396635No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3480.9754730203223545No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3390.9502452697967765No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3320.9306236860546602No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3270.9166082690960056No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3180.8913805185704275No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT3160.8857743517869656No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA3140.8801681850035039No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3130.877365101611773No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3060.8577435178696566No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3010.8437281009110021No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2950.8269096005606167No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2940.8241065171688858No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2850.7988787666433076No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA2760.7736510161177295No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2750.7708479327259986No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2660.7456201822004205No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2560.7175893482831115No Hit
TCCTTGGCTCCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2540.7119831814996496No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT2530.7091800981079187No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2460.6895585143658024No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2450.6867554309740714No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2440.6839523475823406No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2430.6811492641906096No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2350.6587245970567625No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2350.6587245970567625No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2270.6362999299229152No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2250.6306937631394535No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2150.6026629292221444No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA2130.5970567624386826No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1930.5409950946040645No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1920.5381920112123336No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1900.5325858444288718No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1870.524176594253679No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1850.5185704274702173No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1740.48773651016117725No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1730.4849334267694464No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1620.4540995094604064No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1610.45129642606867554No Hit
TCCTTGGCTCCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1590.4456902592852138No Hit
TCCTTGGCTCCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1560.43728100911002105No Hit
TCCTTGGCTCCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1540.4316748423265592No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1530.4288717589348283No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1510.4232655921513665No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1460.40925017519271195No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1450.4064470918009811No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1440.4036440084092502No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1350.37841625788367206No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1290.3615977575332866No Hit
TCCTTGGCTCCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.35038542396636296No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1240.3475823405746321No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1220.3419761737911703No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1170.32796075683251574No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1160.3251576734407849No Hit
TCCTTGGCTCCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1140.31955150665732307No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1130.31674842326559216No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1090.3055360896986685No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1030.2887175893482831No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1010.2831114225648213No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA990.2775052557813595No Hit
TCCTTGGCTCCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT970.2718990889978977No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA970.2718990889978977No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA920.2578836720392432No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA910.25508058864751226No Hit
TCCTTGGCTCCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT900.25227750525578135No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.24947442186405047No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC890.24947442186405047No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT870.24386825508058863No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA870.24386825508058863No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT850.23826208829712683No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT850.23826208829712683No Hit
TCCTTGGCTCCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT830.23265592151366504No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG820.22985283812193413No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC790.2214435879467414No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA790.2214435879467414No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA780.21864050455501052No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.21864050455501052No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA750.21023125437981782No Hit
TCCTTGGCTCCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT750.21023125437981782No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA740.20742817098808689No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA720.2018220042046251No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA720.2018220042046251No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA710.19901892081289418No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA710.19901892081289418No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA690.19341275402943237No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC680.19060967063770148No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA670.18780658724597057No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT660.18500350385423966No Hit
TCCTTGGCTCCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT630.17659425367904694No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA630.17659425367904694No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA600.16818500350385424No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.16538192011212333No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA590.16538192011212333No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.16538192011212333No Hit
TCCTTGGCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT570.15977575332866154No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC570.15977575332866154No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA570.15977575332866154No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT570.15977575332866154No Hit
TCCTTGGCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT570.15977575332866154No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA570.15977575332866154No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.15697266993693063No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.15697266993693063No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA550.15416958654519972No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT550.15416958654519972No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC540.15136650315346883No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.15136650315346883No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT540.15136650315346883No Hit
TCCTTGGCTCCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT530.1485634197617379No Hit
ACTTGCTGAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA510.1429572529782761No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA500.1401541695865452No Hit
TCCTTGGCTCCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT480.1345480028030834No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.1289418360196216No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.12613875262789068No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA450.12613875262789068No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA440.12333566923615978No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA440.12333566923615978No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC430.12053258584442887No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.12053258584442887No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACC420.11772950245269798No Hit
TCCTTGGCTCCGGACTACCAGGGTTTCTAATCCTGTTCGATACCCGCACC420.11772950245269798No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCACGCT420.11772950245269798No Hit
TCCTTGGCTCCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC410.11492641906096707No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC410.11492641906096707No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC400.11212333566923616No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.11212333566923616No Hit
TCCTTGGCTCCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT400.11212333566923616No Hit
TCCTTGGCTCCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC390.10932025227750526No Hit
ACTTGCTGAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA390.10932025227750526No Hit
TCCTTGGCTCCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT390.10932025227750526No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC390.10932025227750526No Hit
TCCTTGGCTCCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT380.10651716888577437No Hit
TCCTTGGCTCCGGACTACGCGGGTATCTAATCCCGTTCGCTCCCCTGGCT380.10651716888577437No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACC380.10651716888577437No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT370.10371408549404344No Hit
TCCTTGGCTCCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT370.10371408549404344No Hit
TCCTTGGCTCCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC360.10091100210231255No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA360.10091100210231255No Hit
ACTTGCTGAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTA360.10091100210231255No Hit
TCCTTGGCTCCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT360.10091100210231255No Hit
TCCTTGGCTCCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT360.10091100210231255No Hit
ACTTGCTGAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTA360.10091100210231255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)