FastQCFastQC Report
Sun 21 May 2023
EGAF00007847680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35814
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8592.3985033785670407No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA8222.2951918244262024No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7882.200256882783269No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA7692.1472050036298653No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT7472.085776511978556No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA7182.0048025911654657No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7121.9880493661696543No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA6651.8568157703691295No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6301.7590886245602277No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT5891.6446082537555147No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA5571.555257720444519No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5531.5440889037806445No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5471.527335678784833No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA5451.5217512704528955No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5331.4882448204612722No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5311.482660412129335No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5191.4491539621377116No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5171.4435695538057742No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA5131.4324007371418999No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4591.2816217121795945No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4221.1783101580387558No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4111.1475959122131012No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4061.133634891383258No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4041.1280504830513207No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3791.0582453789021053No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3631.0135701122466074No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3611.0079857039146702No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3570.9968168872507958No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3460.966102641425141No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3400.9493494164293292No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3380.943765008097392No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3370.9409728039314235No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3350.9353883955994862No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2870.8013625956329926No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2750.7678561456413693No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2710.7566873289774948No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2690.7511029206455576No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT2660.7427263081476517No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT2610.7287652873178087No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2570.7175964706539342No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2440.6812978164963422No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2440.6812978164963422No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2430.6785056123303737No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2280.6366225498408443No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2260.6310381415089071No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT2240.6254537331769698No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2170.6059083040151896No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2170.6059083040151896No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2110.5891550790193779No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1920.5361031998659742No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1890.5277265873680684No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1880.5249343832020997No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1850.516557770704194No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1800.5025967498743509No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1760.49142793321047634No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1700.4746747082146646No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1670.4662980957167588No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1640.45792148321885295No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1620.4523370748869157No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1620.4523370748869157No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1490.4160384207293237No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1470.41045401239738655No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1470.41045401239738655No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1460.40766180823141784No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1400.39090858323560623No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1370.38253197073770034No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1340.3741553582397945No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.3657787457418887No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1290.36019433740995144No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1250.34902552074607696No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1220.3406489082481711No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1170.32668788741832805No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1130.31551907075445357No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1110.3099346624225163No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1100.30714245825654773No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.3015580499246105No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1050.2931814374267046No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1050.2931814374267046No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT1040.29038923326073607No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1040.29038923326073607No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT1020.28480482492879877No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT1000.27922041659686153No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1000.27922041659686153No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT990.27642821243089294No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT980.2736360082649243No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT950.2652593957670185No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT910.25409057910314403No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA880.24571396660523817No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT870.24292176243926955No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT840.23454514994136372No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT830.2317529457753951No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT790.22058412911152062No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA790.22058412911152062No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT790.22058412911152062No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA770.21499972077958343No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT760.2122075166136148No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT740.20662310828167754No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT730.20383090411570892No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT720.2010386999497403No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.1926620874518345No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT670.18707767911989726No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA640.1787010666219914No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.17590886245602277No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC610.17032445412408556No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA600.16753224995811694No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT600.16753224995811694No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.16474004579214832No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC590.16474004579214832No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGATACCCACGCT570.15915563746021108No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT570.15915563746021108No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.15636343329424246No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA560.15636343329424246No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT560.15636343329424246No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGATACCCACGCT550.15357122912827387No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT550.15357122912827387No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC540.15077902496230525No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA540.15077902496230525No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT530.14798682079633663No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT530.14798682079633663No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT520.14519461663036803No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT520.14519461663036803No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT500.13961020829843077No Hit
TCTACAGTTGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA500.13961020829843077No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC490.13681800413246215No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA480.13402579996649355No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT480.13402579996649355No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.13123359580052493No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC470.13123359580052493No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT460.1284413916345563No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA460.1284413916345563No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT460.1284413916345563No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.1284413916345563No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT450.12564918746858772No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT440.12285698330261909No Hit
TCTACAGTTGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA430.12006477913665047No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT430.12006477913665047No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA430.12006477913665047No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC430.12006477913665047No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT430.12006477913665047No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC430.12006477913665047No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGATACCCACGCT420.11727257497068186No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC410.11448037080471324No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT410.11448037080471324No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA400.11168816663874462No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA400.11168816663874462No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.11168816663874462No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACACT390.108895962472776No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA380.1061037583068074No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT380.1061037583068074No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT380.1061037583068074No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT380.1061037583068074No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT370.10331155414083877No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT370.10331155414083877No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC370.10331155414083877No Hit
TCTACAGTTGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA370.10331155414083877No Hit
TCTACAGTTGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA360.10051934997487015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)