FastQCFastQC Report
Sun 21 May 2023
EGAF00007847686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22605
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6542.8931652289316525No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA6242.7604512276045123No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4802.12342402123424No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4592.0305242203052423No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4542.008405220084052No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4521.999557619995576No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG4111.8181818181818181No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT3961.7518248175182483No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT3791.676620216766202No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3701.63680601636806No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3521.5571776155717763No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3111.3758018137580181No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3081.3625304136253042No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2931.2961734129617342No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2861.2652068126520681No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2821.247511612475116No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2250.9953550099535502No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2100.92899800928998No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2090.9245742092457422No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2050.90687900906879No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2050.90687900906879No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2050.90687900906879No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2040.9024552090245521No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2010.889183808891838No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1980.8759124087591241No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1930.8537934085379342No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1920.849369608493696No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1900.8405220084052201No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1820.8051316080513161No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT1760.7785888077858881No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1680.7431984074319841No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1650.7299270072992701No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1640.725503207255032No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1630.7210794072107941No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1590.7033842070338421No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1580.698960406989604No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1530.6768414067684141No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1520.672417606724176No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1510.6679938066799381No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1390.614908206149082No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1340.5927892059278921No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1340.5927892059278921No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1320.583941605839416No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1300.57509400575094No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1220.539703605397036No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1200.53085600530856No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1150.50873700508737No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1140.504313205043132No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1090.4821942048219421No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1080.4777704047777041No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1050.46449900464499No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1030.45565140455651404No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1030.45565140455651404No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1010.4468038044680381No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1010.4468038044680381No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT990.43795620437956206No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA920.40698960406989604No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT900.3981420039814201No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG880.38929440389294406No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT860.38044680380446805No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT860.38044680380446805No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA840.37159920371599203No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG830.367175403671754No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT820.362751603627516No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT760.336208803362088No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC750.33178500331785005No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT750.33178500331785005No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA730.32293740322937403No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT730.32293740322937403No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA730.32293740322937403No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT710.31408980314089807No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA710.31408980314089807No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT710.31408980314089807No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT690.305242203052422No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT690.305242203052422No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT660.291970802919708No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA650.28754700287547No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA650.28754700287547No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA640.28312320283123205No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT630.27869940278699407No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT620.27427560274275603No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC620.27427560274275603No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT620.27427560274275603No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA620.27427560274275603No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA610.269851802698518No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG600.26542800265428No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC580.25658040256580406No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA580.25658040256580406No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT560.247732802477328No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT540.23888520238885205No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA530.23446140234461402No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT520.230037602300376No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT520.230037602300376No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA510.22561380225613803No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT510.22561380225613803No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT490.21676620216766201No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA490.21676620216766201No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT460.20349480203494802No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT460.20349480203494802No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA450.19907100199071004No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC440.19464720194647203No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT440.19464720194647203No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.19022340190223402No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG420.18579960185799602No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC420.18579960185799602No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA420.18579960185799602No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG420.18579960185799602No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT410.181375801813758No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT410.181375801813758No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT410.181375801813758No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT410.181375801813758No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT400.17695200176952003No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT400.17695200176952003No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC390.17252820172528202No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA390.17252820172528202No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT390.17252820172528202No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT390.17252820172528202No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA380.168104401681044No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA370.163680601636806No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT360.15925680159256803No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA360.15925680159256803No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA360.15925680159256803No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT360.15925680159256803No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC360.15925680159256803No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT350.15483300154833002No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT350.15483300154833002No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA350.15483300154833002No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT350.15483300154833002No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT340.150409201504092No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT340.150409201504092No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC340.150409201504092No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTA330.145985401459854No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC330.145985401459854No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA320.14156160141561602No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG320.14156160141561602No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT310.13713780137137802No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA310.13713780137137802No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA300.13271400132714No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT300.13271400132714No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT300.13271400132714No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT290.12829020128290203No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT290.12829020128290203No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC280.123866401238664No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT280.123866401238664No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTA270.11944260119442603No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT270.11944260119442603No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT270.11944260119442603No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT260.115018801150188No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT260.115018801150188No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT260.115018801150188No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA260.115018801150188No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT260.115018801150188No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT260.115018801150188No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGATACCCACGCT260.115018801150188No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT250.11059500110595002No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC250.11059500110595002No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT250.11059500110595002No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT240.106171201061712No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA240.106171201061712No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACC240.106171201061712No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCT240.106171201061712No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC240.106171201061712No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT230.10174740101747401No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT230.10174740101747401No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT230.10174740101747401No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTG230.10174740101747401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)