FastQCFastQC Report
Sun 21 May 2023
EGAF00007847687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22605
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6302.78699402786994No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA5962.6365848263658482No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT5082.2472904224729042No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4882.158814421588144No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4441.9641672196416722No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT4411.9508958195089583No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3631.6058394160583942No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3481.539482415394824No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT3431.5173634151736342No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA3261.4421588144215882No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3111.3758018137580181No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3011.3315638133156382No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT2881.2740544127405442No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2881.2740544127405442No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2741.2121212121212122No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA2651.17230701172307No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2631.1634594116345942No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2591.1457642114576423No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2541.1236452112364521No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT2511.110373811103738No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2381.0528644105286442No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2260.999778809997788No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2230.9865074098650741No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2200.9732360097323601No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2090.9245742092457422No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT1970.8714886087148861No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1940.858217208582172No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT1880.8316744083167441No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1800.7962840079628402No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1690.7476222074762221No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1590.7033842070338421No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1580.698960406989604No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1550.68568900685689No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT1520.672417606724176No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1460.6458748064587481No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1450.64145100641451No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1410.623755806237558No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1410.623755806237558No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1400.6193320061933201No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1340.5927892059278921No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1330.588365405883654No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1290.570670205706702No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1290.570670205706702No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT1280.5662464056624641No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1270.5618226056182261No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1160.5131608051316081No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1150.50873700508737No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1150.50873700508737No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1080.4777704047777041No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1080.4777704047777041No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1070.47334660473346607No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1040.460075204600752No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1030.45565140455651404No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.4468038044680381No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT990.43795620437956206No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA950.42026100420261003No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT930.4114134041141341No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT930.4114134041141341No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT880.38929440389294406No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT860.38044680380446805No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT840.37159920371599203No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.367175403671754No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC820.362751603627516No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG810.35832780358327804No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT810.35832780358327804No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT800.35390400353904006No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT790.349480203494802No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT750.33178500331785005No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT740.327361203273612No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA740.327361203273612No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA740.327361203273612No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA700.30966600309666004No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT670.29639460296394604No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT670.29639460296394604No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG660.291970802919708No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT660.291970802919708No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT640.28312320283123205No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC620.27427560274275603No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT610.269851802698518No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT610.269851802698518No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC610.269851802698518No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG610.269851802698518No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA590.26100420261004204No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG590.26100420261004204No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG570.252156602521566No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA560.247732802477328No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA560.247732802477328No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT560.247732802477328No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA540.23888520238885205No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA540.23888520238885205No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA530.23446140234461402No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAACACGTAAGGGGCGAGCGTTG530.23446140234461402No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT520.230037602300376No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC520.230037602300376No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA510.22561380225613803No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT510.22561380225613803No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT510.22561380225613803No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT500.22119000221190005No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.20791860207918603No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA470.20791860207918603No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA470.20791860207918603No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT450.19907100199071004No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT450.19907100199071004No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG450.19907100199071004No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA450.19907100199071004No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC440.19464720194647203No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT440.19464720194647203No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA440.19464720194647203No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT430.19022340190223402No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT430.19022340190223402No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTA430.19022340190223402No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA410.181375801813758No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC400.17695200176952003No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT390.17252820172528202No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT390.17252820172528202No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT390.17252820172528202No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA370.163680601636806No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT370.163680601636806No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA370.163680601636806No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT360.15925680159256803No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC360.15925680159256803No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA350.15483300154833002No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT340.150409201504092No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC330.145985401459854No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT330.145985401459854No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT330.145985401459854No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT330.145985401459854No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC330.145985401459854No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCTAGCT320.14156160141561602No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA320.14156160141561602No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT320.14156160141561602No Hit
TCTGGTATCGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA310.13713780137137802No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT310.13713780137137802No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC310.13713780137137802No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAACACGTAAGGGGCGAGCGTTG310.13713780137137802No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT300.13271400132714No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT300.13271400132714No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCC290.12829020128290203No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT290.12829020128290203No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT290.12829020128290203No Hit
TCTGGTATCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA290.12829020128290203No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT280.123866401238664No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT280.123866401238664No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC280.123866401238664No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT270.11944260119442603No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACACT270.11944260119442603No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT270.11944260119442603No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA270.11944260119442603No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG260.115018801150188No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT260.115018801150188No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT260.115018801150188No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC260.115018801150188No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT260.115018801150188No Hit
TCTGGTATCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA260.115018801150188No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT250.11059500110595002No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCT250.11059500110595002No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA250.11059500110595002No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT250.11059500110595002No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAACACGTAAGGTGCGAGCGTTG240.106171201061712No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCTAGCT240.106171201061712No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT240.106171201061712No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT240.106171201061712No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT240.106171201061712No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTATTTGCTCCCCACGCT240.106171201061712No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT240.106171201061712No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT230.10174740101747401No Hit
TCTGGTATCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA230.10174740101747401No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT230.10174740101747401No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC230.10174740101747401No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT230.10174740101747401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)