FastQCFastQC Report
Sun 21 May 2023
EGAF00007847699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25334
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA12254.835399068445567No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8433.3275440119996844No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8413.319649482908345No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT6592.601247335596432No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6092.403884108312939No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4631.827583484645141No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA4321.7052182837293755No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4291.693376490092366No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3921.5473277019025815No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3611.424962500986816No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3491.377595326438778No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3351.3223336227994No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3131.2354938027946631No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3001.1841793637009552No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2771.0933922791505486No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2691.0618141627851898No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2581.0183942527828216No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2571.0144469882371516No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2510.9907634009631325No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2510.9907634009631325No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2430.9591852845977737No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2350.9276071682324148No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2220.8762927291387069No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2180.8605036709560275No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT2140.8447146127733481No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2060.8131364964079892No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1970.7776111154969606No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1900.7499802636772717No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1850.7302439409489224No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1840.7262966764032526No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1810.7144548827662429No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1650.6512986500355253No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1640.6473513854898555No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA1630.6434041209441856No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1610.635509591852846No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1610.635509591852846No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1600.6315623273071762No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA1580.6236677982158365No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1540.607878740033157No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1510.5960369463961475No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1510.5960369463961475No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1460.5763006236677982No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1370.5407752427567696No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG1300.5131443909370806No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1260.49735533275440125No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1240.4894608036630615No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG1240.4894608036630615No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1230.4855135391173916No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1160.4578826872977027No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1130.44604089366069316No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA1110.43814636456935346No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1060.41841004184100417No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1050.4144627772953343No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1030.4065682482039946No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1020.4026209836583248No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1020.4026209836583248No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1010.3986737191126549No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT980.3868319254756454No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT960.3789373963843057No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT900.3552538091102866No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA900.3552538091102866No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC890.35130654456461674No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG890.35130654456461674No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT860.33946475092760714No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT830.3276229572905976No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT830.3276229572905976No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA820.32367569274492775No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG810.31972842819925795No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC800.3157811636535881No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA800.3157811636535881No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT780.30788663456224835No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT770.3039393700165785No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA750.2960448409252388No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA740.29209757637956896No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT720.28420304728822926No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT710.2802557827425594No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA690.2723612536512197No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT690.2723612536512197No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC690.2723612536512197No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT680.2684139891055498No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT640.25262493092287047No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT630.24867766637720062No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA630.24867766637720062No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.24473040183153075No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG610.2407831372858609No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA600.23683587274019105No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT590.2328886081945212No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT590.2328886081945212No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA580.22894134364885135No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA580.22894134364885135No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.22894134364885135No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC530.20920502092050208No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA530.20920502092050208No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT530.20920502092050208No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA520.20525775637483223No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC520.20525775637483223No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA520.20525775637483223No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA490.1934159627378227No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC490.1934159627378227No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT490.1934159627378227No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC480.18946869819215284No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA460.18157416910081314No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.18157416910081314No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT460.18157416910081314No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA450.1776269045551433No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA440.17367964000947345No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT440.17367964000947345No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA440.17367964000947345No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA440.17367964000947345No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG430.16973237546380357No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC420.16578511091813372No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC420.16578511091813372No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA410.16183784637246387No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT410.16183784637246387No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT410.16183784637246387No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT410.16183784637246387No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT400.15789058182679405No Hit
TCGAGACGGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA400.15789058182679405No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA400.15789058182679405No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC400.15789058182679405No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG390.15394331728112418No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT390.15394331728112418No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC380.14999605273545433No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT380.14999605273545433No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA380.14999605273545433No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT380.14999605273545433No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT380.14999605273545433No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT380.14999605273545433No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT370.14604878818978448No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT370.14604878818978448No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA370.14604878818978448No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC360.14210152364411463No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT360.14210152364411463No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT350.13815425909844478No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA340.1342069945527749No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCCGGCT340.1342069945527749No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT340.1342069945527749No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC340.1342069945527749No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT330.13025973000710508No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT320.12631246546143524No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA320.12631246546143524No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA320.12631246546143524No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT310.12236520091576537No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT310.12236520091576537No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA310.12236520091576537No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT310.12236520091576537No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT310.12236520091576537No Hit
TCGAGACGGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA310.12236520091576537No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT290.11447067182442568No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT290.11447067182442568No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT290.11447067182442568No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT280.11052340727875581No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT280.11052340727875581No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT280.11052340727875581No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC270.10657614273308597No Hit
TCGAGACGGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA270.10657614273308597No Hit
TCGAGACGGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA270.10657614273308597No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGATACCCACGCT270.10657614273308597No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT270.10657614273308597No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCCGGCT270.10657614273308597No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCC270.10657614273308597No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT260.10262887818741612No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCT260.10262887818741612No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGATACCCACGCT260.10262887818741612No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT260.10262887818741612No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT260.10262887818741612No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT260.10262887818741612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)