FastQCFastQC Report
Sun 21 May 2023
EGAF00007847716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54392
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA32385.953081335490514No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA29055.340858949845566No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA17323.1842918076187674No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA15502.849683777026033No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12042.2135608177673185No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT11302.077511398735108No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG10551.9396234740403No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9661.775996470069128No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9421.7318723341667892No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG9301.7098102662156198No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG9131.6785556699514634No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8651.5903073981467861No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8591.5792763641712018No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7981.4671275187527577No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6871.2630533902044419No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6571.2078982203265187No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6331.16377408442418No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6291.1564200617737903No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6181.1361964994852183No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT6071.1159729371966467No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5601.029563171054567No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG5501.0111781144285923No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT5210.9578614502132666No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5180.9523459332254742No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4870.8953522576849537No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4790.8806442123841742No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4670.8585821444330048No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4630.8512281217826151No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4590.8438740991322253No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4400.8089424915428739No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4400.8089424915428739No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4350.7997499632298868No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4090.7519488160023533No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4070.7482718046771584No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4000.7354022650389763No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3860.7096631857626121No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3460.6361229592587145No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3450.6342844535961171No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3380.621414913957935No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3230.5938373290189733No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2960.5441976761288425No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2870.5276511251654655No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2660.4890425062509193No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2610.479849977937932No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2520.46330342697455507No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2490.4577879099867627No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2400.4412413590233858No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.4375643476981909No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2330.42837181938520374No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2330.42837181938520374No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2300.4228563023974114No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA2260.4155022797470216No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2130.3916017061332549No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2110.38792469480806No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2110.38792469480806No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2100.38608618914546255No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2070.3805706721576702No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA2030.37321664950728045No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1950.35850860420650094No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1950.35850860420650094No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1880.34563906456831883No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1870.3438005589057214No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1820.33460803059273425No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA1820.33460803059273425No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1760.3235769966171496No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1720.31622297396675986No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1650.30335343432857775No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1630.2996764230033829No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1620.2978379173407854No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1530.2812913663774085No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1510.27761435505221355No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1490.27393734372701867No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1490.27393734372701867No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1480.27209883806442126No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1470.2702603324018238No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1410.25922929842623915No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1400.25739079276364174No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1400.25739079276364174No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1400.25739079276364174No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1380.25371378143844686No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1370.2518752757758494No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1370.2518752757758494No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1360.2500367701132519No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1350.24819826445065452No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1340.24635975878805708No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1320.2426827474628622No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1260.23165171348727753No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1250.2298132078246801No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1240.22797470216208265No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1170.21510516252390058No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1160.21326665686130314No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1150.2114281511987057No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1140.20958964553610826No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1140.20958964553610826No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1130.2077511398735108No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1110.20407412854831591No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1100.20223562288571847No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1090.20039711722312104No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1060.19488160023532872No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1040.19120458891013384No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1040.19120458891013384No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1040.19120458891013384No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA1030.1893660832475364No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1010.18568907192234152No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1000.18385056625974408No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT970.17833504927195176No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT970.17833504927195176No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT940.17281953228415942No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT920.16914252095896457No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT900.1654655096337697No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT900.1654655096337697No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT890.16362700397117225No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT850.1562729813207825No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC840.15443447565818502No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT780.14340344168260039No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT770.14156493602000295No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT760.1397264303574055No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT760.1397264303574055No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT750.13788792469480807No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT720.13237240770701575No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT710.1305339020444183No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT680.12501838505662596No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.12317987939402854No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTG670.12317987939402854No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC640.1176643624062362No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC610.11214884541844389No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA600.11031033975584645No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA600.11031033975584645No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT570.10479482276805413No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT560.10295631710545668No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT560.10295631710545668No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA560.10295631710545668No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC550.10111781144285924No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTA550.10111781144285924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)