FastQCFastQC Report
Sun 21 May 2023
EGAF00007847717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54392
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA27405.037505515516988No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA27154.991542873952052No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT13162.419473451978232No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA12592.314678629210178No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT12342.268715987645242No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA12072.219076334755111No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT9501.7465803794675687No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT9381.7245183115163993No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA9011.6564936020002943No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG8911.6381085453743196No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8841.6252390057361379No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT8721.6031769377849683No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG8561.5737608471834093No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA8421.5480217679070452No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG8081.4855125753787322No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT7431.3660097073098985No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7131.3108545374319753No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT6731.2373143109280778No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5771.0608177673187233No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5761.058979261656126No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT5531.0166936314163848No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5350.9836005294896308No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4840.8898367406971613No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4760.8751286953963818No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4650.8549051331078099No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4460.8199735255184587No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4420.8126195028680689No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4410.8107809972054714No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4350.7997499632298868No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA4270.7850419179291073No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4170.7666568613031328No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4120.7574643329901456No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3990.7335637593763789No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3850.7078246801000148No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3680.6765700838358583No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3670.6747315781732608No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3640.6692160611854685No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3640.6692160611854685No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3370.6195764082953376No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3300.6067068686571555No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3210.5901603176937785No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT3080.5662597440800118No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2950.542359170466245No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2890.5313281364906604No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2880.529489630828063No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2880.529489630828063No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2860.5258126195028681No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2820.5184585968524783No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2770.5092660685394911No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2740.5037505515516988No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2710.49823503456390644No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2650.48720400058832175No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2530.4651419326371525No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2280.4191792910722165No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2240.4118252684218267No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2050.37689366083247533No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2040.3750551551698779No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2020.37137814384468304No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2020.37137814384468304No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT2010.3695396381820856No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1980.3640241211942933No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1970.3621856155316958No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1960.3603471098690984No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1860.34196205324312395No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA1860.34196205324312395No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA1820.33460803059273425No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1820.33460803059273425No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1780.3272540079423445No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1770.325415502279747No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1750.32173849095455215No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1740.31989998529195474No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1710.3143844683041624No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1690.3107074569789675No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1680.30886895131637004No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1650.30335343432857775No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1640.3015149286659803No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1480.27209883806442126No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1460.2684218267392264No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1450.2665833210766289No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA1410.25922929842623915No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1410.25922929842623915No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1410.25922929842623915No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1340.24635975878805708No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1340.24635975878805708No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1290.23716723047506988No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1290.23716723047506988No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA1290.23716723047506988No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1240.22797470216208265No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1230.22613619649948521No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA1220.22429769083688778No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1200.2206206795116929No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1170.21510516252390058No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1160.21326665686130314No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1150.2114281511987057No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1150.2114281511987057No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA1130.2077511398735108No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA1120.20591263421091335No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1120.20591263421091335No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA1110.20407412854831591No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1100.20223562288571847No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1080.1985586115605236No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1060.19488160023532872No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT1040.19120458891013384No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT1010.18568907192234152No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC960.1764965436093543No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT930.17098102662156198No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA920.16914252095896457No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT910.16730401529636713No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT910.16730401529636713No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT890.16362700397117225No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT890.16362700397117225No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT890.16362700397117225No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT870.15994999264597737No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT870.15994999264597737No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.15994999264597737No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCT820.15075746433299014No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT780.14340344168260039No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT770.14156493602000295No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA770.14156493602000295No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC760.1397264303574055No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC760.1397264303574055No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT760.1397264303574055No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC740.13604941903221063No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA730.1342109133696132No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT730.1342109133696132No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT730.1342109133696132No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT720.13237240770701575No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC720.13237240770701575No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCT710.1305339020444183No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTCGCTCCCCATGCT710.1305339020444183No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT700.12869539638182087No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC700.12869539638182087No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT680.12501838505662596No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT630.11582585674363877No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA620.11398735108104133No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT620.11398735108104133No Hit
TGGATAGAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA620.11398735108104133No Hit
TGGATAGAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA620.11398735108104133No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT610.11214884541844389No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT600.11031033975584645No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC600.11031033975584645No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT580.10663332843065157No Hit
TGGATAGAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA580.10663332843065157No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT580.10663332843065157No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA580.10663332843065157No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.10479482276805413No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC560.10295631710545668No Hit
TGGATAGAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA550.10111781144285924No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT550.10111781144285924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)