FastQCFastQC Report
Sun 21 May 2023
EGAF00007847720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48156
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA22364.643242794252014No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA18303.800149514079242No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG9902.0558185895838523No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG9191.9083810947753137No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG8721.8107816263809287No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT8631.792092366475621No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT8561.7775562754381593No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8491.7630201844006976No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7311.5179832211977737No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG7051.4639920259157737No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG6981.449455934878312No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA6681.3871584018606196No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT6681.3871584018606196No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6461.3414735443143118No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6371.322784284409004No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT6371.322784284409004No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG5521.1462746075255419No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5321.1047429188470803No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA4861.0092200348866185No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4550.9448459174350029No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4530.9406927485671568No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT4470.9282332419636182No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT4330.8991610598886951No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4200.8721654622476951No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4150.8617825400780796No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4080.847246449040618No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3580.7434172273444639No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA3480.722651383005233No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3410.7081152919677715No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.6935792009303098No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA3310.6873494476285406No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA3240.672813356591079No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3160.6562006811196943No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3040.6312816679126173No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2890.6001329014037711No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2830.5876733948002325No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2770.5752138881966941No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2740.5689841348949248No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2730.5669075504610018No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2670.5544480438574633No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2600.5399119528200017No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2530.5253758617825401No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA2530.5253758617825401No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2490.5170695240468477No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2400.49838026414153996No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2320.4817675886701553No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2250.4672314976326937No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT2250.4672314976326937No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2230.46307832876484756No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA2220.46100174433092456No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2150.4464656532934629No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT2130.4423124844256167No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2130.4423124844256167No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2030.42154664008638587No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2010.4173934712185397No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1920.398704211313232No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1860.3862447047096935No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1850.3841681202757704No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1850.3841681202757704No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1750.36340227593653956No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1740.3613256915026165No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1730.35924910706869345No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1710.3550959382008472No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1710.3550959382008472No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1670.3467896004651549No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1550.3218705872580779No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1530.31771741839023177No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1490.3094110806545394No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1470.30525791178669326No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.30525791178669326No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA1440.299028158484924No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1430.2969515740510009No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1420.2948749896170778No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1370.2844920674474624No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1360.28241548301353936No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1340.2782623141456932No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1340.2782623141456932No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1330.2761857297117701No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1280.2658028075421547No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1260.26164963867430846No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1250.2595730542403854No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1250.2595730542403854No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1240.25749646980646235No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1220.2533433009386162No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1190.24711354763684693No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1160.24088379433507764No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1160.24088379433507764No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1120.23257745659938533No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1110.23050087216546228No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1060.22011794999584686No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCAAGCGTTG1050.21804136556192377No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT1050.21804136556192377No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1010.20973502782623143No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT1010.20973502782623143No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1000.20765844339230835No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA960.199352105656616No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT960.199352105656616No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT960.199352105656616No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA950.19727552122269293No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT930.19312235235484676No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC930.19312235235484676No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT930.19312235235484676No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC920.19104576792092365No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT910.1889691834870006No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATGCGTAGGGTGCGAGCGTTA910.1889691834870006No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT890.1848160146191544No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT880.18273943018523134No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT880.18273943018523134No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT870.18066284575130825No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC870.18066284575130825No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA870.18066284575130825No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA860.17858626131738517No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC850.17650967688346209No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA850.17650967688346209No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA840.174433092449539No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT830.17235650801561592No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT820.17027992358169283No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC800.16612675471384666No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA780.16197358584600052No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA780.16197358584600052No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA770.1598970014120774No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT740.15366724811030816No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT730.15159066367638507No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA710.1474374948085389No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCT710.1474374948085389No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT710.1474374948085389No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA710.1474374948085389No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT700.14536091037461585No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCATGCT690.14328432594069274No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT690.14328432594069274No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTG670.1391311570728466No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA670.1391311570728466No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT660.13705457263892348No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT660.13705457263892348No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACC650.13497798820500043No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC650.13497798820500043No Hit
ATTATCGTGGTGTGTCAGCAGCCGCGGTAATGCGTAGGGTGCGAGCGTTA640.13290140377107734No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA640.13290140377107734No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA640.13290140377107734No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTCGCTCCCCATGCT630.13082481933715423No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT630.13082481933715423No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT630.13082481933715423No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA620.12874823490323117No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTA610.1266716504693081No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT610.1266716504693081No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTCGATACCCGCACC600.12459506603538499No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT600.12459506603538499No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCT570.11836531273361575No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT570.11836531273361575No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA570.11836531273361575No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC570.11836531273361575No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA560.11628872829969267No Hit
ATTATCGTGGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA560.11628872829969267No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC560.11628872829969267No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT550.11421214386576957No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT550.11421214386576957No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC550.11421214386576957No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC540.1121355594318465No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTG530.11005897499792343No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT530.11005897499792343No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC520.10798239056400033No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT520.10798239056400033No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC510.10590580613007725No Hit
ATTATCGTGGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA510.10590580613007725No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA500.10382922169615418No Hit
ATTATCGTGGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCCGGCGTTA490.10175263726223108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)