FastQCFastQC Report
Sun 21 May 2023
EGAF00007847751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39357
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA11602.947379119343446No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA11552.934674899001448No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA8382.1292273293188No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7902.0072668140356225No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7741.9666133089412303No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA7431.8878471428208452No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT7051.791295068221663No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6991.776050003811266No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6481.6464669563228906No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6051.537210661381711No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT6041.5346698173133115No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5481.3923825494829383No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5431.3796783291409407No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5041.2805854104733592No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5021.2755037223365602No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT4871.2373910613105674No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT4851.2323093731737682No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG4811.2221459969001702No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG4361.1078080138221917No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4311.0951037934801942No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT4061.031582691770206No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3941.0010925629494118No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3860.9807658104022156No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3710.9426531493762227No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA3580.909622176487029No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA3530.8969179561450314No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT3510.8918362680082323No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3340.8486419188454405No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3230.8206926340930457No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3220.8181517900246462No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA3070.7800391289986534No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA3000.7622532205198567No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2930.7444673120410601No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2810.7139771832202658No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2800.7114363391518662No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2800.7114363391518662No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT2790.7088954950834667No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2730.6936504306730696No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT2680.6809462103310719No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT2430.6174251086210839No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT2380.6047208882790863No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2270.5767716035266915No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2220.564067383184694No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2180.5539040069110959No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT2170.5513631628426964No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2140.5437406306374978No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT2120.5386589425006988No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA2040.5183321899535026No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1940.4929237492695073No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1890.48021952892750974No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1880.4776786848591102No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT1860.47259699672231115No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1860.47259699672231115No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1850.4700561526539116No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1700.43194349162791873No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1690.4294026475595193No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT1670.42432095942272025No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1650.41923927128592114No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1640.41669842721752165No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1630.4141575831491221No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1590.40399420687552406No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA1580.4014533628071245No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1510.3836674543283279No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT1510.3836674543283279No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1490.37858576619152884No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT1490.37858576619152884No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA1470.37350407805472974No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1460.37096323398633024No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1450.36842238991793075No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1430.3633407017811317No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA1420.3607998577127322No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1410.35825901364433266No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA1380.3506364814391341No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT1370.34809563737073457No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1360.345554793302335No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1350.3430139492339355No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1350.3430139492339355No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1340.34047310516553597No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1320.335391417028737No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT1310.33285057296033743No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1280.3252280407551389No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1260.3201463526183398No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1220.30998297634474176No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1220.30998297634474176No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1200.30490128820794266No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1200.30490128820794266No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1120.28457453566074653No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1080.27441115938714844No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1070.2718703153187489No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1030.2617069390451508No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1000.2540844068399522No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC970.24646187463475366No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC960.24392103056635417No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT930.23629849836115557No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT880.22359427801915796No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA880.22359427801915796No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.22105343395075844No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA870.22105343395075844No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT870.22105343395075844No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT860.21851258988235894No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA850.21597174581395936No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC840.21343090174555987No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT840.21343090174555987No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA820.20834921360876082No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA770.1956449932667632No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC750.19056330512996417No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT750.19056330512996417No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.18802246106156467No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT740.18802246106156467No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA720.1829407729247656No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT720.1829407729247656No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC710.1803999288563661No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA690.17531824071956706No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA670.17023655258276799No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC670.17023655258276799No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA660.1676957085143685No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA660.1676957085143685No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.16515486444596894No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.16261402037756945No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTCGATACCCGCACC630.1600731763091699No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.15753233224077037No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC620.15753233224077037No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA610.15499148817237088No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT610.15499148817237088No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT600.15245064410397133No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.14990980003557183No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT590.14990980003557183No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCT570.14482811189877276No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCT560.14228726783037327No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA530.1346647356251747No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT530.1346647356251747No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT530.1346647356251747No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCT510.12958304748837565No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA500.1270422034199761No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA500.1270422034199761No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT500.1270422034199761No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA500.1270422034199761No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACT480.12196051528317708No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT480.12196051528317708No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT480.12196051528317708No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC480.12196051528317708No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT480.12196051528317708No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCCGTTCGCTCCCCTGGCT470.11941967121477755No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA470.11941967121477755No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT460.11687882714637803No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACT460.11687882714637803No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT460.11687882714637803No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT450.11433798307797849No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT450.11433798307797849No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTA450.11433798307797849No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTCGATACCCGCACC450.11433798307797849No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCT450.11433798307797849No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCTAGCT440.11179713900957898No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA440.11179713900957898No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG430.10925629494117947No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCT430.10925629494117947No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAACACGTAGGGTGCGAGCGTTA420.10671545087277994No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT420.10671545087277994No Hit
AATCCGTTAGTGTGTCAGCAGCCGCGGTAACACGTAGGGTGCGAGCGTTA420.10671545087277994No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT410.10417460680438041No Hit
AATCCGTTAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA410.10417460680438041No Hit
AATCCGTTAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTA410.10417460680438041No Hit
AATCCGTTAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA410.10417460680438041No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCT410.10417460680438041No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT400.10163376273598088No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACC400.10163376273598088No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCT400.10163376273598088No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCCGTTCGCTCCCCTGGCT400.10163376273598088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)