FastQCFastQC Report
Sun 21 May 2023
EGAF00007847753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43874
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21714.948260929024023No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA21024.790992387290879No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA14323.263892054519761No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14243.2456580206956285No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12472.8422300223366914No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT10452.3818206682773395No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8501.9373660938141042No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA8181.864429958517573No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT8141.8553129416055067No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA7491.7071614167844282No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6871.5658476546473992No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6791.5476136208232667No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6231.4199753840543374No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5551.2649860965492092No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5361.2216802662168937No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT5261.198887723936728No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5111.1646989105164791No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT4961.13051009709623No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4891.114555317500114No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA4691.068970232939782No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4421.0074303687833341No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4080.9299357250307699No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4000.9117016912066372No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4000.9117016912066372No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3990.9094224369786207No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG3890.8866298946984547No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT3840.8752336235583716No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT3740.8524410812782058No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA3640.8296485389980398No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT3570.8136937594019237No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3540.8068559967178739No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3010.6860555226329945No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT2920.6655422345808452No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2910.6632629803528286No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2880.6564252176687788No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2650.6040023704243972No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA2620.5971646077403474No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT2570.5857683366002644No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2540.5789305739162146No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA2540.5789305739162146No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2500.5698135570041483No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG2460.5606965400920818No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG2410.5493002689519989No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA2310.526507726671833No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2240.5105529470757169No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC2200.5014359301636504No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2110.48092264211150115No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2100.4786433878834845No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA2070.4718056251994347No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA2020.4604093540593518No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1970.4490130829192688No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1830.41710352372703646No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1790.4079865068149701No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1710.38975247299083743No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1680.3829147103067876No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1680.3829147103067876No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1660.3783562018507544No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG1660.3783562018507544No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1660.3783562018507544No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1650.37607694762273786No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1580.3601221680266217No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1480.33732962574645575No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1450.33049186306240597No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1450.33049186306240597No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1430.3259333546063728No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTA1410.3213748461503396No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1400.319095591922323No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT1400.319095591922323No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1360.3099785750102566No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1290.2940237954141405No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.2917445411861239No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1230.2803482700460409No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1230.2803482700460409No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC1210.27578976159000773No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1160.2643934904499248No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1110.2529972193098418No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1100.2507179650818252No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1100.2507179650818252No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTA1080.24615945662579206No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1070.24388020239777547No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1060.24160094816975888No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1030.23476318548570907No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG1030.23476318548570907No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT990.22564616857364272No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT950.21652915166157632No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT940.21424989743355974No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC920.20969138897752654No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT910.20741213474950995No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG890.20285362629347678No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA890.20285362629347678No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG880.2005743720654602No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA880.2005743720654602No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT870.1982951178374436No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT860.19601586360942697No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT860.19601586360942697No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA850.1937366093814104No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA830.1891781009253772No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA830.1891781009253772No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC800.18234033824132745No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT770.17550257555727766No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC770.17550257555727766No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT760.17322332132926108No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.17322332132926108No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA750.1709440671012445No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC750.1709440671012445No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC740.16866481287322788No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC740.16866481287322788No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC730.1663855586452113No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACACT720.1641063044171947No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA690.15726854173314492No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT670.15271003327711172No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA650.14815152482107855No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTCGATACCCGCACC630.14359301636504537No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.14359301636504537No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA630.14359301636504537No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA630.14359301636504537No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT620.14131376213702876No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT600.1367552536809956No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCTCAAGCGTTA590.134475999452979No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA580.1321967452249624No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT570.1299174909969458No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTCGATACCCGCACC560.12763823676892921No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT550.1253589825409126No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT550.1253589825409126No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCT550.1253589825409126No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT540.12307972831289603No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA530.12080047408487944No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT530.12080047408487944No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA530.12080047408487944No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA520.11852121985686283No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA520.11852121985686283No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC520.11852121985686283No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA510.11624196562884624No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT510.11624196562884624No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACACT510.11624196562884624No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA500.11396271140082966No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT490.11168345717281304No Hit
AATGCCAATGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTA480.10940420294479646No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT480.10940420294479646No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA480.10940420294479646No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC470.10712494871677987No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT470.10712494871677987No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA460.10484569448876327No Hit
AATGCCAATGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA460.10484569448876327No Hit
AATGCCAATGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA460.10484569448876327No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA460.10484569448876327No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT450.10256644026074668No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTCGATACCCGCACC440.1002871860327301No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACC440.1002871860327301No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCTCAAGCGTTA440.1002871860327301No Hit
AATGCCAATGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA440.1002871860327301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)