FastQCFastQC Report
Sun 21 May 2023
EGAF00007847756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50036
Sequences flagged as poor quality0
Sequence length250
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA22494.494763770085538No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA21404.276920617155648No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA16253.2476616835878165No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA14702.9378847229994407No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT12332.4642257574546327No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT12102.4182588536253897No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT10132.0245423295227436No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9751.94859701015269No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA9031.804700615556799No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT8671.7327524182588534No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7771.55288192501399No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG7381.4749380446078824No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6901.3790071148772884No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6741.3470301383004237No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6341.267087696858262No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA6291.2570948916779918No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6261.2510992085698298No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT6051.2091294268126949No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5841.16715964505556No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA5721.1431769126229114No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT5551.1092013750099927No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5191.0372531777120473No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4690.9373251259093452No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4650.929330881765129No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG4590.9173395155488049No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTA4590.9173395155488049No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG4480.8953553441522105No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4470.8933567831161563No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4450.8893596610440483No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4390.8773682948277242No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4380.8753697337916699No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4040.8074186585658326No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG3990.7974258533855625No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA3880.775441681988968No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG3540.7074906067631306No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3500.6994963626189143No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3440.6875049964025901No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3410.681509313294428No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA3400.679510752258374No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3340.6675193860420497No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3330.6655208250059957No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3160.631545287393077No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3030.6055639939243744No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT2980.5955711887441042No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2890.5775841394196178No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2650.5296186745543209No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA2550.5096330641937805No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA2540.5076345031577265No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG2400.4796546486529699No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2400.4796546486529699No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA2330.4656647214005916No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA2270.4536733551842673No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2250.44967623311215926No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2200.4396834279318891No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2180.43568630585978096No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2180.43568630585978096No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2080.4157006954992406No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT2080.4157006954992406No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2000.39971220721080825No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT1890.37772803581421377No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT1890.37772803581421377No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1860.37173235270605165No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1860.37173235270605165No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1840.3677352306339436No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1810.3617395475257814No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1770.3537453033815653No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1740.34774962027340317No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1670.3337596930210249No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1660.3317611319849708No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1650.32976257094891676No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1630.3257654488768087No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1570.31377408266048445No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA1560.3117755216244304No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1540.30777839955232233No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1540.30777839955232233No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1480.2957870333359981No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1440.2877927891917819No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1430.28579422815572786No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1400.27979854504756574No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA1390.2777999840115117No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1370.2738028619394037No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA1370.2738028619394037No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1320.26381005675913344No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1290.2578143736509713No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1290.2578143736509713No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1230.24582300743464705No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1210.24182588536253896No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA1180.23583020225437684No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1110.22184027500199857No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1080.21584459189383642No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT1080.21584459189383642No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.2098489087856743No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1020.2038532256775122No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1010.20185466464145815No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA940.18786473738907986No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC910.18186905428091774No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA890.17787193220880967No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC890.17787193220880967No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA870.17387481013670159No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA870.17387481013670159No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT870.17387481013670159No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT860.17187624910064753No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC860.17187624910064753No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA850.1698776880645935No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT820.16388200495643135No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT800.1598848828843233No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT780.1558877608122152No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT760.15189063874010714No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT760.15189063874010714No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA760.15189063874010714No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT760.15189063874010714No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC720.14389639459589096No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT710.14189783355983693No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT690.13790071148772884No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.1359021504516748No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA670.13390358941562075No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA670.13390358941562075No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCATGCT660.13190502837956672No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT660.13190502837956672No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC660.13190502837956672No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC650.12990646734351266No Hit
AACATGCTCGTGTGCCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA640.12790790630745863No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT630.1259093452714046No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT620.12391078423535054No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT610.1219122231992965No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCATGCT610.1219122231992965No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC590.11791510112718842No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCT580.11591654009113438No Hit
AACATGCTCGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA580.11591654009113438No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA570.11391797905508033No Hit
AACATGCTCGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTG560.11191941801902631No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT560.11191941801902631No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC550.10992085698297227No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC550.10992085698297227No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTG540.10792229594691821No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCT540.10792229594691821No Hit
AACATGCTCGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA530.10592373491086417No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT520.10392517387481015No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT510.1019266128387561No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCT510.1019266128387561No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGCTCCCCATGCT510.1019266128387561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)