FastQCFastQC Report
Sun 21 May 2023
EGAF00007847766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55185
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA27154.91981516716499No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA26364.776660324363505No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA20073.636857841804838No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA19833.5933677629790703No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT13542.453565280420404No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT12682.297725831294736No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT10891.9733623267192173No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT10131.835643743770952No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA9761.7685965389145601No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8841.60188457008245No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT7301.3228232309504393No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA6881.2467155930053457No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT6811.2340309866811634No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA6661.2068496874150585No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA6581.1923529944731357No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6521.1814804747666938No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT6331.1470508290296275No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG6281.137990395940926No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA6221.127117876234484No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6181.1198695297635226No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG5971.0818157107909758No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT5821.054634411524871No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT5480.9930234665216997No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA5370.9730905137265562No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA5270.9549696475491528No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA5150.933224608136269No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT5140.9314125215185286No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5100.9241641750475673No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5050.9151037419588657No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT5040.9132916553411252No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA5010.9078553954879044No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4960.8987949623992026No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT4910.889734529310501No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA4890.8861103560750203No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4710.8534927969556945No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT4460.8081906315121863No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT4170.7556401195977167No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3960.7175863006251699No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3880.7030896076832472No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT3600.6523511823865181No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3600.6523511823865181No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3550.6432907492978164No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3450.6251698831204131No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3370.6106731901784905No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3170.574431457823684No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT2940.5327534656156564No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2800.5073842529672918No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2750.4983238198785902No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2520.4566458276705626No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2520.4566458276705626No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2440.44214913472864004No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2400.4349007882576787No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2380.43127661502219805No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2330.4222161819334964No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG2290.41496783546253513No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT2160.3914107094319108No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1950.35335689045936397No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1930.3497327172238833No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT1910.34610854398840263No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT1850.3352360242819607No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1800.32617559119325906No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT1720.31167889825133643No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1710.3098668116335961No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1710.3098668116335961No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1680.3044305517803751No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1630.29537011869167346No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1600.28993385883845246No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1570.2844975989852315No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1560.2826855123674912No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1560.2826855123674912No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1550.2808734257497508No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1410.25550421310138627No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1370.24825586663042493No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1370.24825586663042493No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1350.2446316933949443No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1310.237383346923983No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1300.23557126030624262No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1280.23194708707076198No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1260.2283229138352813No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1250.22651082721754098No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1220.22107456736432002No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1220.22107456736432002No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT1210.21926248074657967No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1200.21745039412883935No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1180.2138262208933587No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1120.20295370118691672No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1110.2011416145691764No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT1100.19932952795143608No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1050.19026909486273444No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1030.1866449216272538No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1020.18483283500951345No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT1010.18302074839177312No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC1000.1812086617740328No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC990.17939657515629248No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT960.1739603153030715No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT940.17033614206759085No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT910.16489988221436985No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA890.16127570897888918No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT880.15946362236114886No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC870.15765153574340854No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC860.15583944912566822No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT860.15583944912566822No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA830.15040318927244722No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA820.1485911026547069No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT820.1485911026547069No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA810.14677901603696658No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC780.1413427561837456No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT770.13953066956600527No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA740.13409440971278427No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT730.13228232309504395No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA720.13047023647730363No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA720.13047023647730363No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC710.12865814985956328No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA690.12503397662408264No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC690.12503397662408264No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT680.1232218900063423No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT670.12140980338860197No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGATACCCGCACC670.12140980338860197No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT650.11778563015312131No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGGGGGTGCGAGCGTTA650.11778563015312131No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA650.11778563015312131No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA640.11597354353538099No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA640.11597354353538099No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTCGATACCCGCACC640.11597354353538099No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA630.11416145691764065No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA630.11416145691764065No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCT630.11416145691764065No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC620.11234937029990033No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTCGCTCCCCATGCT610.11053728368216001No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC600.10872519706441967No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA600.10872519706441967No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCT590.10691311044667935No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT590.10691311044667935No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA590.10691311044667935No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA590.10691311044667935No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGATACCCACGCT580.10510102382893902No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCT570.1032889372111987No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA560.10147685059345836No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT560.10147685059345836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)