FastQCFastQC Report
Sun 21 May 2023
EGAF00007847767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007847767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55185
Sequences flagged as poor quality0
Sequence length250
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA25824.678807647005527No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA25084.544713237292743No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTA18293.31430642384706No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTA17233.1222252423665853No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCT15512.8105463441152487No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCT13772.4952432726284317No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCT11001.9932952795143608No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCT10991.9914831928966203No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCT8611.5602065778744223No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA8011.4514813808100027No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA7761.4061792153664945No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA7191.3028902781552958No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCT7131.292017758448854No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCT7121.2902056718311135No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA6831.237655159916644No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCT6791.2304068134456827No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCT6721.2177222071215004No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA6401.1597354353538099No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA6111.1071849234393405No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG6051.0963124037328984No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG5791.0491981516716498No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTA5531.0020838996104013No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTA5500.9966476397571804No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA5420.9821509468152578No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCT5390.9767146869620368No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCT5260.9531575609314126No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA5080.9205400018120866No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACAAGCGTTA4870.8824861828395397No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTA4840.8770499229863187No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCT4820.8734257497508381No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCT4680.8480565371024734No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCT4680.8480565371024734No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCT4580.8299356709250703No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTATGTCACAAGCGTTA4490.8136268913654073No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTA4400.7973181118057443No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTG4270.77376098577512No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCT3790.6867808281235843No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTA3730.6759083084171423No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTG3630.6577874422397391No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCT3590.6505390957687778No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCT3480.6306061429736342No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCT3440.6233577965026729No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCT3430.6215457098849325No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG3410.6179215366494519No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTA3240.5871160641478663No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCT3110.563558938117242No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCT3090.5599347648817613No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG3080.5581226782640211No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCT2790.5055721663495516No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCT2760.5001359064963306No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCT2560.46389417414152395No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCT2460.4457733079641207No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTG2330.4222161819334964No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTG2280.4131557488447948No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCT2180.3950348826673915No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCT2110.3823502763432092No Hit
TCCTAACAACCGGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCT2050.37147775663676724No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGATACCCGCACC1930.3497327172238833No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCT1910.34610854398840263No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1890.342484370752922No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1840.33342393766422035No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCACGCT1770.32073933134003807No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1760.3189272447222977No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCT1750.3171151581045574No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCT1670.3026184651626348No Hit
TCCTAACAACCGGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCT1630.29537011869167346No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGATACCCGCACC1580.2863096856029718No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGATACCCGCACC1550.2808734257497508No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1540.27906133913201053No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1510.27362507927878954No Hit
TCCTAACAACCGGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCT1500.2718129926610492No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGATACCCGCACC1470.2663767328078282No Hit
TCCTAACAACCGGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCT1470.2663767328078282No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCACGCT1450.26275255957234755No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTTGATACCCACGCT1450.26275255957234755No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1410.25550421310138627No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCATGCT1340.24281960677720393No Hit
TCCTAACAACCGGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1300.23557126030624262No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA1300.23557126030624262No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTA1300.23557126030624262No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTG1240.22469874059980066No Hit
TCCTAACAACCGGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCT1240.22469874059980066No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTG1220.22107456736432002No Hit
TCCTAACAACCGGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCT1200.21745039412883935No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCACGCT1150.2083899610401377No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1140.2065778744223974No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCT1120.20295370118691672No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1080.1957053547159554No Hit
TCCTAACAACCGGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCT1070.19389326809821508No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA1070.19389326809821508No Hit
TCCTAACAACCGGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCT1070.19389326809821508No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT1070.19389326809821508No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTA1050.19026909486273444No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTA1040.18845700824499412No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA1000.1812086617740328No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCATGCT1000.1812086617740328No Hit
TCCTAACAACCGGACTACGCGGGTTTCTAATCCCGTTCGCTCCCCTGGCT970.1757724019208118No Hit
TCCTAACAACCGGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCT970.1757724019208118No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGAGGATACGAGCGTTA970.1757724019208118No Hit
TCCTAACAACCGGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCT960.1739603153030715No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA950.17214822868533117No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTA920.16671196883211017No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCT920.16671196883211017No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTTGATACCCACGCT910.16489988221436985No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCGAGCGTTA890.16127570897888918No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGATACCCGCACC880.15946362236114886No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTATGTCGCAAGCGTTA860.15583944912566822No Hit
TCCTAACAACCGGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACC860.15583944912566822No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTCGATACCCGCACC830.15040318927244722No Hit
TCCTAACAACCGGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCT820.1485911026547069No Hit
TCCTAACAACCGGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACC820.1485911026547069No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACC810.14677901603696658No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCGAGCGTTA780.1413427561837456No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTTGATACCCACGCT770.13953066956600527No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT740.13409440971278427No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTTGATACCCACGCT720.13047023647730363No Hit
TCCTAACAACCGGACTACTGGGGTATCTAATCCTGTTCGATACCCGCACC720.13047023647730363No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTCGATACCCGCACC700.12684606324182296No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCT700.12684606324182296No Hit
TCCTAACAACCGGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCT700.12684606324182296No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCTGTTTGATACCCACGCT690.12503397662408264No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT680.1232218900063423No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTA680.1232218900063423No Hit
AAGGTTCAAGTGTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTA670.12140980338860197No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCCGTTCGCTCCCCTGGCT640.11597354353538099No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGGAGGATACGAGCGTTA640.11597354353538099No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTA630.11416145691764065No Hit
TCCTAACAACCGGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCATGCT630.11416145691764065No Hit
TCCTAACAACCGGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCATGCT620.11234937029990033No Hit
TCCTAACAACCGGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACC620.11234937029990033No Hit
TCCTAACAACCGGACTACCGGGGTTTCTAATCCTGTTTGATACCCACGCT600.10872519706441967No Hit
TCCTAACAACCGGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCT600.10872519706441967No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTA600.10872519706441967No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGGGGGGTGCGAGCGTTA590.10691311044667935No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA590.10691311044667935No Hit
AAGGTTCAAGTGTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTA590.10691311044667935No Hit
TCCTAACAACCGGACTACACGGGTTTCTAATCCTGTTTGATACCCACGCT580.10510102382893902No Hit
AAGGTTCAAGTGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTA570.1032889372111987No Hit
AAGGTTCAAGTGTGTCAGCCGCCGCGGTAATACGTATGTCGCAAGCGTTA560.10147685059345836No Hit
TCCTAACAACCGGACTACAGGGGTTTCTAATCCCGTTCGCTCCCCTGGCT560.10147685059345836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)